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A magnetic nanoparticle-based lateral flow immunochromatography assay for the rapid detection of fluoroquinolones in milk

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Abstract

A magnetic nanoparticle (MNP)-based lateral flow immunochromatography assay (LFIA) was established to detect fluoroquinolones (FQs) in milk. This method is an improvement of traditional Au nanoparticle (AuNP)-based LFIA. Compared with AuNP-labeled antibody, the combination between MNPs and antibody is stronger and more stable, and the preparation process is convenient. In this study, the color intensity of MNPs-LFIA test strip was analyzed, which laid a research foundation for magnetic quantitative analysis and high sensitivity analysis of LFIA. The limits of detection (LODs) for 10 FQs in phosphate buffer (PB) solution ranged from 1 to 5 ng/mL, and in milk ranged from 2 to 20 ng/mL. The recovery in spiked milk sample ranged from 16.47 to 83.67%. The log functions of analyte concentration and T/C value were fitted into a linear equation. The specificity of the MNPs-LFIA is satisfactory, without cross-reaction with antibiotics from other chemical classes. The results of this method are consistent with those of ultra-performance liquid chromatography-mass spectrometry, indicating the MNPs-LFIA method is reliable and accurate. As far as we know, this is the first time that MNPs-LIFA has been applied to the detection of FQs in milk.

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Acknowledgements

The authors gratefully thank the National Science Foundation of China (No. 31372482), Project of Tianjin dairy (mutton sheep) industry technology system innovation team construction (ITTCRS2021000), Project of Tianjin “131” innovative talent team (20180318) and Project of Tianjin postgraduate research innovation (2019YJSS094) for financial support.

Funding

This work was supported by the National Natural Science Foundation of China (31372482), Project of Tianjin dairy (mutton sheep) industry technology system innovation team construction (ITTCRS2021000), Project of Tianjin “131” innovative talent team (20180318) and Project of Tianjin postgraduate research innovation (2019YJSS094).

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Authors and Affiliations

  1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China

    Chang Liu, Linyan Yang, Wei Zhang, Daowen Li, Liuan Li, Hongyu Wang & Cun Li

  2. Tianjin Academy of Agricultural Science, Tianjin, 300381, China

    Yi Ma

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  1. Chang Liu
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  2. Linyan Yang
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  3. Wei Zhang
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  4. Daowen Li
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Correspondence to Yi Ma or Cun Li.

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Chang Liu declares that she has no conflict of interest. Linyan Yang declares that she has no conflict of interest. Wei Zhang declares that she has no conflict of interest. Daowen Li declares that he has no conflict of interest. Liuan Li declares that he has no conflict of interest. Hongyu Wang declares that he has no conflict of interest. Yi Ma declares that she has no conflict of interest. Cun Li declares that he has no conflict of interest.

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Liu, C., Yang, L., Zhang, W. et al. A magnetic nanoparticle-based lateral flow immunochromatography assay for the rapid detection of fluoroquinolones in milk. Eur Food Res Technol 247, 2645–2656 (2021). https://doi.org/10.1007/s00217-021-03820-z

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  • DOI: https://doi.org/10.1007/s00217-021-03820-z

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