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Physicochemical characterization of honeys from Brazilian monitored beehives

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Abstract

This study was performed with floral honeys from an innovator and pioneer project in Brazil for the digital monitoring of beehives. Samples from six regions of the Santa Catarina state were evaluated for moisture, acidity, pH, electrical conductivity, 5-HMF, diastase activity, fructose, glucose, sucrose, and minerals (K, Ca, Na, Mg, and Mn). Furthermore, chemometric analyzes were applied to classify honeys based on these parameters. Some results did not corroborate with the current legislation for free acidity (≤ 50 mEq kg−1) and electrical conductivity (≤ 0.8 mS cm−1). In all samples, the minerals K (0.37 × 103 to 3.03 × 103 mg kg−1) and Ca (0.03 × 103 to 0.38 × 103 mg kg−1) were majority. Considering that the results were different (p < 0.05) for all floral honey samples, cluster and principal components analysis (PCA) were performed to group samples by similarity. Cluster analysis provided a first separation of the honeys and PCA highlighted the K, Ca, Mg, and Na as the best contributors for honeys discrimination. In this regard, the physicochemical parameters, sugar, and mineral content were important to elucidate the characteristics of honeys. They indicated that the beehives handling and the hygienic-sanitary conditions during the collection and transport of samples were also adequate. Furthermore, mineral elements were proposed to discriminate honey samples produced in different regions of Santa Catarina state, Brazil.

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The authors declare that all data are presented in the manuscript in Figures and Tables. The background data, if required, will be made available on request.

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References

  1. Brasil. Ministério da Agricultura Pecuária e Abastecimento. Instrução Normativa nº 11, de 20 de outubro de 2000. Regulamento técnico de identidade e qualidade do mel. Diário Oficial, Brasília, 20 de outubro de 2000. Available from: http://extranet.agricultura.gov.br/sislegisconsulta/consultarLegislacao.do?operacao=visualizar&id=7797.  Accessed 10 June 2021

  2. European Comission (2002) European Commission Council Directive 2001/110/EC of 20 December 2001 relating to honey. Off J Eur Commun 10:47

    Google Scholar 

  3. Codex Alimentarius (2001) Revised Codex Standard for Honey, Standards and Standard Methods. In: Codex Alimentarius Commission FAO/OMS. Available from http://www.fao.org/fao-who-codexalimentarius/sh-proxy/en/?lnk=1&url=https%253A%252F%252Fworkspace.fao.org%252Fsites%252Fcodex%252FStandards%252FCXS%2B12-1981%252FCXS_012e.pdf  Accessed 5 June 2021

  4. Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R (2016) Honey: chemical composition, stability and authenticity. Food Chem 196:309–323. https://doi.org/10.1016/j.foodchem.2015.09.051

    Article  CAS  PubMed  Google Scholar 

  5. De-Melo AAM, de Almeida-Muradian LB, Sancho MT, Pascual-Maté A (2017) Composition and properties of Apis mellifera honey: a review. J Apic Res 57(1):5–37. https://doi.org/10.1080/00218839.2017.1338444

    Article  Google Scholar 

  6. Seraglio SKT, da Silva B, Bergamo G, Brugnerotto P, Gonzaga LV, Fett R, et al. (2019) An overview of physicochemical characteristics and health-promoting properties of honeydew honey. Food Res Int. 119:44–66. https://doi.org/10.1016/j.foodres.2019.01.028

    Article  CAS  PubMed  Google Scholar 

  7. IBGE (2018) Tabela 74 - Produção de origem animal por tipo de produto - Ano 2018. Instituto Brasileiro de Geografia e Estatísitica. Available from: https://sidra.ibge.gov.br/tabela/74#resultado. Accessed 1 Feb 2021

  8. Epagri (2018) Tecnologia para o desenvolvimento da Apicultura Catarinense. Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina. p. 1–2. Available from: https://ciram.epagri.sc.gov.br/ciram_arquivos/apicultura/acervo/folder_apis_on_line.pdf. Accessed 10 Feb 2021

  9. Thomé VMR, Zampieri S, Braga HJ, Pandolfo C, da Silva Júnior VP, Laus Neto J, Soldateli D, Gebler E, Ore JD, Echeverria L et al (1999) Zoneamento Agroecológico e Socioeconômico de Santa Catarina. Epagri, Florianópolis, p 1000

    Google Scholar 

  10. AOAC (2005). Official methods of analysis of AOAC International, 18th edn. AOAC International, Gaithersburg. Available from https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/#:~:text=Official%20Methods%20of%20Analysis%20(OMA,of%20more%203%2C000%20validated%20methods.&text=Official%20Methods%20of%20Analysis%20(OMA)%20is%20the%20most%20comprehensive%20and,methods%20and%20consensus%20standards%20available. Accessed 5 June 2021

  11. Bogdanov S, Lüllmann C, Martin P, Von Der Ohe W, Russmann H, Vorwohl G et al (1999) Honey quality and international regulatory standards: review by the International Honey Commission. Bee World 80(2):61–69. https://doi.org/10.1080/0005772X.1999.11099428

    Article  Google Scholar 

  12. Rizelio VM, Tenfen L, da Silveira R, Gonzaga LV, Costa ACO, Fett R (2012) Development of a fast capillary electrophoresis method for determination of carbohydrates in honey samples. Talanta. 93:62–6. https://doi.org/10.1016/j.talanta.2012.01.034

    Article  CAS  PubMed  Google Scholar 

  13. Rizelio VM, Gonzaga LV, Borges GDSC, França Maltez H, Costa ACO, Fett R (2012) Fast determination of cations in honey by capillary electrophoresis: A possible method for geographic origin discrimination. Talanta. 99:450–6. https://doi.org/10.1016/j.talanta.2012.06.009

    Article  CAS  Google Scholar 

  14. Nguyen HTL, Panyoyai N, Paramita VD, Mantri N, Kasapis S (2018) Physicochemical and viscoelastic properties of honey from medicinal plants. Food Chem 241(March 2017):143–149. https://doi.org/10.1016/j.foodchem.2017.08.070

    Article  CAS  PubMed  Google Scholar 

  15. Sakač MB, Jovanov PT, Marić AZ, Pezo LL, Kevrešan ŽS, Novaković AR et al (2019) Physicochemical properties and mineral content of honey samples from Vojvodina (Republic of Serbia). Food Chem 27: 15–21. https://doi.org/10.1016/j.foodchem.2018.09.149

    Article  CAS  PubMed  Google Scholar 

  16. Oroian M, Ropciuc S (2017) Honey authentication based on physicochemical parameters and phenolic compounds. Comput Electron Agric 138:148–156. https://doi.org/10.1016/j.compag.2017.04.020

    Article  Google Scholar 

  17. Nayik GA, Dar BN, Nanda V (2015) Physico-chemical, rheological and sugar profile of different unifloral honeys from Kashmir valley of India. Arab J Chem. https://doi.org/10.1016/j.arabjc.2015.08.017

    Article  Google Scholar 

  18. Karabagias IK, Louppis AP, Karabournioti S, Kontakos S, Papastephanou C, Kontominas MG (2017) Characterization and geographical discrimination of commercial Citrus spp. honeys produced in different Mediterranean countries based on minerals, volatile compounds and physicochemical parameters, using chemometrics. Food Chem 217:445–455. https://doi.org/10.1016/j.foodchem.2016.08.124

    Article  CAS  PubMed  Google Scholar 

  19. Pereira JR, da Campos RAN, de Oliveira FC, Silva VRO, David GF, Da Silva JG et al (2020) Physical-chemical characterization of commercial honeys from Minas Gerais, Brazil. Food Biosci 36:1–10. https://doi.org/10.1016/j.fbio.2020.100644

    Article  CAS  Google Scholar 

  20. Mračević SĐ, Krstić M, Lolić A, Ražić S (2020) Comparative study of the chemical composition and biological potential of honey from different regions of Serbia. Microchem J 152:1–9. https://doi.org/10.1016/j.microc.2019.104420

    Article  CAS  Google Scholar 

  21. Bergamo G, Seraglio SKT, Gonzaga LV, Fett R, Costa ACO (2019) Physicochemical characteristics of bracatinga honeydew honey and blossom honey produced in the state of Santa Catarina: An approach to honey differentiation. Food Res Int. 116:745–54. https://doi.org/10.1016/j.foodres.2018.09.007

    Article  CAS  Google Scholar 

  22. Fechner DC, Hidalgo MJ, Ruiz Díaz JD, Gil RA, Pellerano RG (2020) Geographical origin authentication of honey produced in Argentina. Food Biosci 33(February 2019):100483. https://doi.org/10.1016/j.fbio.2019.100483

    Article  CAS  Google Scholar 

  23. Brugnerotto P, Della Betta F, Gonzaga LV, Fett R, Oliveira Costa AC (2019) A capillary electrophoresis method to determine aliphatic organic acids in bracatinga honeydew honey and floral honey. J Food Compos Anal. https://doi.org/10.1016/j.jfca.2019.103243

    Article  Google Scholar 

  24. Codex Alimentarius Commission (2001) Codex Alimentarius Commission Standards. Codex Stan 12-1981. 1–8

  25. Geană EI, Ciucure CT, Costinel D, Ionete RE (2020) Evaluation of honey in terms of quality and authenticity based on the general physicochemical pattern, major sugar composition and δ13C signature. Food Control 109:106919. https://doi.org/10.1016/j.foodcont.2019.106919

    Article  CAS  Google Scholar 

  26. Chakir A, Romane A, Ferrazzi P, Luigi G (2016) Physicochemical properties of some honeys produced from different plants in Morocco. Arab J Chem 9:946–954. https://doi.org/10.1016/j.arabjc.2011.10.013

    Article  CAS  Google Scholar 

  27. Rodríguez I, Cámara-Martos F, Flores JM, Serrano S (2019) Spanish avocado (Persea americana Mill.)honey: authentication based on its composition criteria, mineral content and sensory attributes. Lwt 111(February):561–572. https://doi.org/10.1016/j.lwt.2019.05.068

    Article  CAS  Google Scholar 

  28. Fechner DC, Moresi AL, Ruiz Díaz JD, Pellerano RG, Vazquez FA (2016) Multivariate classification of honeys from corrientes (Argentina) according to geographical origin based on physicochemical properties. Food Biosci 15:49–54. https://doi.org/10.1016/j.fbio.2016.05.002

    Article  CAS  Google Scholar 

  29. Bergamo G, Tischer Seraglio SK, Gonzaga LV, Fett R, Costa ACO (2018) Mineral profile as a potential parameter for verifying the authenticity of bracatinga honeydew honeys. LWT 97:390–395. https://doi.org/10.1016/j.lwt.2018.07.028

    Article  CAS  Google Scholar 

  30. Kaygusuz H, Tezcan F, Erim FB, Yildiz O, Sahin H, Can Z et al (2016) Characterization of Anatolian honeys based on minerals, bioactive components and principal component analysis. LWT Food Sci Technol 68:273–279. https://doi.org/10.1016/j.lwt.2015.12.005

    Article  CAS  Google Scholar 

  31. Karabagias IK, Louppis AP, Kontakos S, Papastephanou C, Kontominas MG (2017) Characterization and geographical discrimination of Greek pine and thyme honeys based on their mineral content, using chemometrics. Eur Food Res Technol 243(1):101–113. https://doi.org/10.1007/s00217-016-2727-8

    Article  CAS  Google Scholar 

  32. Rizelio VM, Gonzaga LV, Da Silva Campelo Borges G, Micke GA, Fett R, Costa ACO (2012) Development of a fast MECK method for determination of 5-HMF in honey samples (c). Food Chem 133(4):1640–1645. https://doi.org/10.1016/j.foodchem.2011.11.058

    Article  CAS  Google Scholar 

  33. da Silva PM, Gonzaga LV, Biluca FC, Schulz M, Vitali L, Micke GA, et al. (2020) Stability of Brazilian Apis mellifera L. honey during prolonged storage: Physicochemical parameters and bioactive compounds. LWT. 129:109521. https://doi.org/10.1016/j.lwt.2020.109521

    Article  CAS  Google Scholar 

  34. Rodríguez-Flores MS, Escuredo O, Míguez M, Seijo MC (2019) Differentiation of oak honeydew and chestnut honeys from the same geographical origin using chemometric methods. Food Chem 297:124979. https://doi.org/10.1016/j.foodchem.2019.124979

    Article  CAS  PubMed  Google Scholar 

  35. Bystrzanowska M, Tobiszewski M (2020) Chemometrics for selection, prediction, and classification of sustainable solutions for green chemistry—a review. Symmetry (Basel) 12(12):1–21. https://doi.org/10.3390/sym12122055

    Article  Google Scholar 

  36. Karabagias IK, Vlasiou M, Kontakos S, Drouza C, Kontominas MG, Keramidas AD (2018) Geographical discrimination of pine and fir honeys using multivariate analyses of major and minor honey components identified by1H NMR and HPLC along with physicochemical data. Eur Food Res Technol 244(7):1249–1259. https://doi.org/10.1007/s00217-018-3040-5

    Article  CAS  Google Scholar 

  37. Azevedo MS, Seraglio SKT, Rocha G, Balderas CB, Piovezan M, Gonzaga LV et al (2017) Free amino acid determination by GC-MS combined with a chemometric approach for geographical classification of bracatinga honeydew honey (Mimosa scabrella Bentham). Food Control 78:383–392. https://doi.org/10.1016/j.foodcont.2017.03.008

    Article  CAS  Google Scholar 

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Funding

This study was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Financial code—001), the Fundação de Amparo à Pesquisa e Inovação do estado de Santa Catarina (FAPESC) (No. 88887.178578/2018-00), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (No 150371/2019-5, No. 160175/2019-4, and No. 153639/2019-9).

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Authors and Affiliations

  1. Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, 88034-001, Brazil

    Patricia Brugnerotto, Bibiana Silva, Siluana Katia Tischer Seraglio, Mayara Schulz, Luciano Valdemiro Gonzaga, Roseane Fett & Ana Carolina Oliveira Costa

  2. Agricultural Research and Rural Extension Company of Santa Catarina (Epagri)/Santa Catarina Environmental Resources and Hydrometeorology Information Center (Ciram), Florianópolis, SC, 88034-901, Brazil

    Everton Blainski & Denilson Dortzbach

  3. Centro de Ciências Agrárias, Departamento de Ciência E Tecnologia de Alimentos, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346, Itacorubi, Florianópolis, SC, 88034-000, Brazil

    Patricia Brugnerotto & Ana Carolina Oliveira Costa

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  1. Patricia Brugnerotto
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  2. Bibiana Silva
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  3. Siluana Katia Tischer Seraglio
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  7. Luciano Valdemiro Gonzaga
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Correspondence to Patricia Brugnerotto or Ana Carolina Oliveira Costa.

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Brugnerotto, P., Silva, B., Seraglio, S.K.T. et al. Physicochemical characterization of honeys from Brazilian monitored beehives. Eur Food Res Technol 247, 2709–2719 (2021). https://doi.org/10.1007/s00217-021-03805-y

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