Abstract
This study investigates the impact of wet fermentation on flavor volatiles and sensory quality of coffee beans and beverage. Pulped coffee beans were kept underwater for 36 h before air-drying and roasting, and volatiles in the beans were characterized by solid-phase microextraction and gas chromatography–mass spectrometry. A total of 79 volatiles were identified. Fermented roasted beans had higher concentrations of ethanol, isoamyl alcohol, 3-methylbutanal, benzaldehyde, acetaldehyde, and ethyl acetate compared with mechanically processed (no fermentation) beans; whereas, ketones, pyrazines, pyrroles, pyridines, furans and sulfides were not significantly different between the two treatments. Coffee made with fermented beans was rated significantly higher in flavor, aroma, acidity, body and uniformity scores with noted fruity aroma compared with beverage made with mechanically processed beans in sensory evaluation (cup test) by a 3 Q-Grade Coffee Certification Panel. The findings demonstrated wet fermentation played an important role in coffee flavor, aroma and sensory quality.
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References
International Coffee Organization (2018) Total production by all exporting countries (in thousand 60 kg bags). https://www.ico.org/prices/po-production.pdf. Accessed 23 Aug 2020
Buffo RA, Cardelli-Freire C (2004) Coffee flavour: an overview. Flavour Fragr J 19(2):99–104
Silva C, Schwan RF, Dias ËS, Wheals AE (2000) Microbial diversity during maturation and natural processing of coffee cherries of Coffea arabica in Brazil. Int J Food Microbiol 60(2–3):251–260
Knopp S, Bytof G, Selmar D (2006) Influence of processing on the content of sugars in green Arabica coffee beans. Eur Food Res Technol 223(2):195
Avallone S, Guiraud JP, Guyot B, Olguin E, Brillouet JM (2001) Fate of mucilage cell wall polysaccharides during coffee fermentation. J Agric Food Chem 49(11):5556–5559
Pereira GVdM, de Carvalho Neto DP, Medeiros ABP, Soccol VT, Neto E, Woiciechowski AL, Soccol CR (2016) Potential of lactic acid bacteria to improve the fermentation and quality of coffee during on-farm processing. Int J Food Sci Technol 51(7):1689–1695
Pereira GVdM, Soccol VT, Brar SK, Neto E, Soccol CR (2017) Microbial ecology and starter culture technology in coffee processing. Crit Rev Food Sci Nutr 57(13):2775–2788
Amorim HV, Amorim VL (1977) Coffee enzymes and coffee quality. In: Ory RL, St Angelo AJ (eds) Enzymes in food and beverages processing, vol 47. American Chemical Society, pp 27–56. https://doi.org/10.1021/bk-1977-0047.ch003
Avallone S, Guyot B, Brillouet J-M, Olguin E, Guiraud J-P (2001) Microbiological and biochemical study of coffee fermentation. Curr Microbiol 42(4):252–256
Frank HA, Lum NA, Cruz ASD (1965) Bacteria responsible for mucilage-layer decomposition in Kona coffee cherries. Appl Environ Microbiol 13(2):201–207
Suárez-Quiroz ML, González-Rios O, Barel M, Guyot B, Schorr-Galindo S, Guiraud JP (2004) Effect of chemical and environmental factors on Aspergillus ochraceus growth and toxigenesis in green coffee. Food Microbiol 21(6):629–634
Gonzalez-Rios O, Suarez-Quiroz ML, Boulanger R, Barel M, Guyot B, Guiraud J-P, Schorr-Galindo S (2007) Impact of “ecological” post-harvest processing on the volatile fraction of coffee beans: I. Green coffee. J Food Compos Anal 20(3–4):289–296
Bodner M, Morozova K, Kruathongsri P, Thakeow P, Scampicchio M (2019) Effect of harvesting altitude, fermentation time and roasting degree on the aroma released by coffee powder monitored by proton transfer reaction mass spectrometry. Eur Food Res Technol 245(7):1499–1506
Haile M, Kang W (2019) The role of microbes in coffee fermentation and their impact on coffee quality. J Food Qual. https://doi.org/10.1155/2019/4836709
Ribeiro LS, Evangelista SR, Miguel MGdCP, van Mullem J, Silva CF, Schwan RF (2018) Microbiological and chemical-sensory characteristics of three coffee varieties processed by wet fermentation. Ann Microbiol 68(10):705–716
Elhalis H, Cox J, Zhao J (2020) Ecological diversity, evolution and metabolism of microbial communities in the wet fermentation of Australian coffee beans. Int J Food Microbiol 321:108544
Frank DC, Geesink G, Alvarenga TIRC, Polkinghorne R, Stark J, Lee M, Warner R (2017) Impact of high oxygen and vacuum retail ready packaging formats on lamb loin and topside eating quality. Meat Sci 123:126–133
Buyukpamukcu E, Goodall DM, Hansen C-E, Keely BJ, Kochhar S, Wille H (2001) Characterization of peptides formed during fermentation of cocoa bean. J Agric Food Chem 49(12):5822–5827
Kumazawa K, Masuda H (2003) Investigation of the change in the flavor of a coffee drink during heat processing. J Agric Food Chem 51(9):2674–2678
Swiegers JH, Bartowsky EJ, Henschke PA, Pretorius IS (2005) Yeast and bacterial modulation of wine aroma and flavour. Aust J Grape Wine Res 11(2):139–173
Carballo J (2012) The role of fermentation reactions in the generation of flavor and aroma of foods. In: Mehta BM, Afaf K-E, Iwanski RZ (eds) Fermentation: effects on food properties. CRC Press, Boca Raton, pp 51–83
Ho VTT, Zhao J, Fleet G (2014) Yeasts are essential for cocoa bean fermentation. Int J Food Microbiol 174:72–87
Pires EJ, Teixeira JA, Brányik T, Vicente AA (2014) Yeast: the soul of beer’s aroma—a review of flavour-active esters and higher alcohols produced by the brewing yeast. Appl Microbiol Biotechnol 98(5):1937–1949
Liu SQ, Pilone GJ (2000) An overview of formation and roles of acetaldehyde in winemaking with emphasis on microbiological implications. Int J Food Sci Technol 35(1):49–61
Mukisa IM, Byaruhanga YB, Muyanja CMBK, Langsrud T, Narvhus JA (2017) Production of organic flavor compounds by dominant lactic acid bacteria and yeasts from Obushera, a traditional sorghum malt fermented beverage. Int J Food Sci Nutr 5(3):702–712
Pereira GVdM, Neto E, Soccol VT, Medeiros ABP, Woiciechowski AL, Soccol CR (2015) Conducting starter culture-controlled fermentations of coffee beans during on-farm wet processing: growth, metabolic analyses and sensorial effects. Food Res Int 75:348–356
Jost P, Piendl A (1976) Technological influences on the formation of acetate during fermentation. J Am Soc Brew Chem 34(1):31–37
Vasserot Y, Mornet F, Jeandet P (2010) Acetic acid removal by Saccharomyces cerevisiae during fermentation in oenological conditions. Metabolic consequences. Food Chem 119(3):1220–1223
Bertrand B, Boulanger R, Dussert S, Ribeyre F, Berthiot L, Descroix F, Joët T (2012) Climatic factors directly impact the volatile organic compound fingerprint in green Arabica coffee bean as well as coffee beverage quality. Food Chem 135(4):2575–2583
Saerens S, Swiegers JH (2016) Production of low-alcohol or alcohol-free beer with Pichia kluyveri yeast strains. US Patent 10415007, 17 Sep 2019
Peddie HAB (1990) Ester formation in brewery fermentations. J Inst Brew 96(5):327–331
Matsumoto K, Yanagi R, Oe Y (2018) Recent advances in the synthesis of carboxylic acid esters. Intech Open, London
Besson I, Creuly C, Gros JB, Larroche C (1997) Pyrazine production by Bacillus subtilis in solid-state fermentation on soybeans. Appl Microbiol Biotechnol 47(5):489–495
Czerny M, Grosch W (2000) Potent odorants of raw Arabica coffee. Their changes during roasting. J Agric Food Chem 48(3):868–872
Daglia M, Papetti A, Aceti C, Sordelli B, Spini V, Gazzani G (2007) Isolation and determination of α-dicarbonyl compounds by RP-HPLC-DAD in green and roasted coffee. J Agric Food Chem 55(22):8877–8882
Ludwig E, Lipke U, Raczek U, Jäger A (2000) Investigations of peptides and proteases in green coffee beans. Eur Food Res Technol 211(2):111–116
De Bruyn F, Zhang SJ, Pothakos V, Torres J, Lambot C, Moroni AV, Callanan M, Sybesma W, Weckx S, De Vuyst L (2017) Exploring the impacts of postharvest processing on the microbiota and metabolite profiles during green coffee bean production. Appl Environ Microbiol 83(1):e02398-e12316
Yu A-N, Zhang A-D (2010) The effect of pH on the formation of aroma compounds produced by heating a model system containing l-ascorbic acid with l-threonine/l-serine. Food Chem 119(1):214–219
Liu C, Yang Q, Linforth R, Fisk ID, Yang N (2019) Modifying Robusta coffee aroma by green bean chemical pre-treatment. Food Chem 272:251–257
Blank I, Sen A, Grosch W (1991) Aroma impact compounds of Arabica and Robusta coffee. Qualitative and quantitative investigations. In: 14th International Scientific Colloquium on coffee, San Francisco, 1991. ASIC, Paris, pp 117–129
Sunarharum WB, Williams DJ, Smyth HE (2014) Complexity of coffee flavor: A compositional and sensory perspective. Food Res Int 62:315–325
Flament I, Gautschi F, Winter M, Willhalm B, Stoll M (1968) Les composants furanniques de l’arome de café: quelques aspects chimiques et spectroscopiques, 1968. Association Scientifique Internationale du Café, París (Francia)
Pereira GVdM, Soccol VT, Pandey A, Medeiros ABP, Lara JMRA, Gollo AL, Soccol CR (2014) Isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process. Int J Food Microbiol 188:60–66
Febrianto NA, Yang TA, Wan Abdullah WA (2016) Cocoa-like flavor compound development of Rambutan seed fat as the effect of fermentation and roasting. Int Food Res J 23(5):2166–2174
Mottram R (2005) The LRI and odour database. https://www.odour.org.uk/lriindex.html. Accessed 23 Jan 2020
Acknowledgements
The authors gratefully acknowledge Mr. Benji Salim Ang, Director of The Q Coffee Trading, for assisting in preparing the coffee beans and arranging the sensory evaluation tests.
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HE designed and conducted experiments, perform the data analysis and wrote the manuscript. JZ and DF supervised the experiments progress, interpreted the scientific values of the obtained data and proofread the manuscript. JC supervised work. All authors read and approved the manuscript.
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This experiment was conducted at the University of New South Wales Sydney with human ethics approval by the HREAP Executives (HC Number HC190689).
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Elhalis, H., Cox, J., Frank, D. et al. The role of wet fermentation in enhancing coffee flavor, aroma and sensory quality. Eur Food Res Technol 247, 485–498 (2021). https://doi.org/10.1007/s00217-020-03641-6
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DOI: https://doi.org/10.1007/s00217-020-03641-6