Skip to main content
Log in

The role of wet fermentation in enhancing coffee flavor, aroma and sensory quality

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

This study investigates the impact of wet fermentation on flavor volatiles and sensory quality of coffee beans and beverage. Pulped coffee beans were kept underwater for 36 h before air-drying and roasting, and volatiles in the beans were characterized by solid-phase microextraction and gas chromatography–mass spectrometry. A total of 79 volatiles were identified. Fermented roasted beans had higher concentrations of ethanol, isoamyl alcohol, 3-methylbutanal, benzaldehyde, acetaldehyde, and ethyl acetate compared with mechanically processed (no fermentation) beans; whereas, ketones, pyrazines, pyrroles, pyridines, furans and sulfides were not significantly different between the two treatments. Coffee made with fermented beans was rated significantly higher in flavor, aroma, acidity, body and uniformity scores with noted fruity aroma compared with beverage made with mechanically processed beans in sensory evaluation (cup test) by a 3 Q-Grade Coffee Certification Panel. The findings demonstrated wet fermentation played an important role in coffee flavor, aroma and sensory quality.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

Data availability

Not applicable.

Code availability

Not applicable.

References

  1. International Coffee Organization (2018) Total production by all exporting countries (in thousand 60 kg bags). https://www.ico.org/prices/po-production.pdf. Accessed 23 Aug 2020

  2. Buffo RA, Cardelli-Freire C (2004) Coffee flavour: an overview. Flavour Fragr J 19(2):99–104

    Article  CAS  Google Scholar 

  3. Silva C, Schwan RF, Dias ËS, Wheals AE (2000) Microbial diversity during maturation and natural processing of coffee cherries of Coffea arabica in Brazil. Int J Food Microbiol 60(2–3):251–260

    Article  Google Scholar 

  4. Knopp S, Bytof G, Selmar D (2006) Influence of processing on the content of sugars in green Arabica coffee beans. Eur Food Res Technol 223(2):195

    Article  CAS  Google Scholar 

  5. Avallone S, Guiraud JP, Guyot B, Olguin E, Brillouet JM (2001) Fate of mucilage cell wall polysaccharides during coffee fermentation. J Agric Food Chem 49(11):5556–5559

    Article  CAS  Google Scholar 

  6. Pereira GVdM, de Carvalho Neto DP, Medeiros ABP, Soccol VT, Neto E, Woiciechowski AL, Soccol CR (2016) Potential of lactic acid bacteria to improve the fermentation and quality of coffee during on-farm processing. Int J Food Sci Technol 51(7):1689–1695

    Article  CAS  Google Scholar 

  7. Pereira GVdM, Soccol VT, Brar SK, Neto E, Soccol CR (2017) Microbial ecology and starter culture technology in coffee processing. Crit Rev Food Sci Nutr 57(13):2775–2788

    Article  Google Scholar 

  8. Amorim HV, Amorim VL (1977) Coffee enzymes and coffee quality. In: Ory RL, St Angelo AJ (eds) Enzymes in food and beverages processing, vol 47. American Chemical Society, pp 27–56. https://doi.org/10.1021/bk-1977-0047.ch003

  9. Avallone S, Guyot B, Brillouet J-M, Olguin E, Guiraud J-P (2001) Microbiological and biochemical study of coffee fermentation. Curr Microbiol 42(4):252–256

    CAS  PubMed  Google Scholar 

  10. Frank HA, Lum NA, Cruz ASD (1965) Bacteria responsible for mucilage-layer decomposition in Kona coffee cherries. Appl Environ Microbiol 13(2):201–207

    Article  CAS  Google Scholar 

  11. Suárez-Quiroz ML, González-Rios O, Barel M, Guyot B, Schorr-Galindo S, Guiraud JP (2004) Effect of chemical and environmental factors on Aspergillus ochraceus growth and toxigenesis in green coffee. Food Microbiol 21(6):629–634

    Article  Google Scholar 

  12. Gonzalez-Rios O, Suarez-Quiroz ML, Boulanger R, Barel M, Guyot B, Guiraud J-P, Schorr-Galindo S (2007) Impact of “ecological” post-harvest processing on the volatile fraction of coffee beans: I. Green coffee. J Food Compos Anal 20(3–4):289–296

    Article  CAS  Google Scholar 

  13. Bodner M, Morozova K, Kruathongsri P, Thakeow P, Scampicchio M (2019) Effect of harvesting altitude, fermentation time and roasting degree on the aroma released by coffee powder monitored by proton transfer reaction mass spectrometry. Eur Food Res Technol 245(7):1499–1506

    Article  CAS  Google Scholar 

  14. Haile M, Kang W (2019) The role of microbes in coffee fermentation and their impact on coffee quality. J Food Qual. https://doi.org/10.1155/2019/4836709

    Article  Google Scholar 

  15. Ribeiro LS, Evangelista SR, Miguel MGdCP, van Mullem J, Silva CF, Schwan RF (2018) Microbiological and chemical-sensory characteristics of three coffee varieties processed by wet fermentation. Ann Microbiol 68(10):705–716

    Article  CAS  Google Scholar 

  16. Elhalis H, Cox J, Zhao J (2020) Ecological diversity, evolution and metabolism of microbial communities in the wet fermentation of Australian coffee beans. Int J Food Microbiol 321:108544

    Article  CAS  Google Scholar 

  17. Frank DC, Geesink G, Alvarenga TIRC, Polkinghorne R, Stark J, Lee M, Warner R (2017) Impact of high oxygen and vacuum retail ready packaging formats on lamb loin and topside eating quality. Meat Sci 123:126–133

    Article  CAS  Google Scholar 

  18. Buyukpamukcu E, Goodall DM, Hansen C-E, Keely BJ, Kochhar S, Wille H (2001) Characterization of peptides formed during fermentation of cocoa bean. J Agric Food Chem 49(12):5822–5827

    Article  CAS  Google Scholar 

  19. Kumazawa K, Masuda H (2003) Investigation of the change in the flavor of a coffee drink during heat processing. J Agric Food Chem 51(9):2674–2678

    Article  CAS  Google Scholar 

  20. Swiegers JH, Bartowsky EJ, Henschke PA, Pretorius IS (2005) Yeast and bacterial modulation of wine aroma and flavour. Aust J Grape Wine Res 11(2):139–173

    Article  CAS  Google Scholar 

  21. Carballo J (2012) The role of fermentation reactions in the generation of flavor and aroma of foods. In: Mehta BM, Afaf K-E, Iwanski RZ (eds) Fermentation: effects on food properties. CRC Press, Boca Raton, pp 51–83

    Google Scholar 

  22. Ho VTT, Zhao J, Fleet G (2014) Yeasts are essential for cocoa bean fermentation. Int J Food Microbiol 174:72–87

    Article  CAS  Google Scholar 

  23. Pires EJ, Teixeira JA, Brányik T, Vicente AA (2014) Yeast: the soul of beer’s aroma—a review of flavour-active esters and higher alcohols produced by the brewing yeast. Appl Microbiol Biotechnol 98(5):1937–1949

    Article  CAS  Google Scholar 

  24. Liu SQ, Pilone GJ (2000) An overview of formation and roles of acetaldehyde in winemaking with emphasis on microbiological implications. Int J Food Sci Technol 35(1):49–61

    Article  CAS  Google Scholar 

  25. Mukisa IM, Byaruhanga YB, Muyanja CMBK, Langsrud T, Narvhus JA (2017) Production of organic flavor compounds by dominant lactic acid bacteria and yeasts from Obushera, a traditional sorghum malt fermented beverage. Int J Food Sci Nutr 5(3):702–712

    CAS  Google Scholar 

  26. Pereira GVdM, Neto E, Soccol VT, Medeiros ABP, Woiciechowski AL, Soccol CR (2015) Conducting starter culture-controlled fermentations of coffee beans during on-farm wet processing: growth, metabolic analyses and sensorial effects. Food Res Int 75:348–356

    Article  CAS  Google Scholar 

  27. Jost P, Piendl A (1976) Technological influences on the formation of acetate during fermentation. J Am Soc Brew Chem 34(1):31–37

    CAS  Google Scholar 

  28. Vasserot Y, Mornet F, Jeandet P (2010) Acetic acid removal by Saccharomyces cerevisiae during fermentation in oenological conditions. Metabolic consequences. Food Chem 119(3):1220–1223

    Article  CAS  Google Scholar 

  29. Bertrand B, Boulanger R, Dussert S, Ribeyre F, Berthiot L, Descroix F, Joët T (2012) Climatic factors directly impact the volatile organic compound fingerprint in green Arabica coffee bean as well as coffee beverage quality. Food Chem 135(4):2575–2583

    Article  CAS  Google Scholar 

  30. Saerens S, Swiegers JH (2016) Production of low-alcohol or alcohol-free beer with Pichia kluyveri yeast strains. US Patent 10415007, 17 Sep 2019

  31. Peddie HAB (1990) Ester formation in brewery fermentations. J Inst Brew 96(5):327–331

    Article  CAS  Google Scholar 

  32. Matsumoto K, Yanagi R, Oe Y (2018) Recent advances in the synthesis of carboxylic acid esters. Intech Open, London

    Book  Google Scholar 

  33. Besson I, Creuly C, Gros JB, Larroche C (1997) Pyrazine production by Bacillus subtilis in solid-state fermentation on soybeans. Appl Microbiol Biotechnol 47(5):489–495

    Article  CAS  Google Scholar 

  34. Czerny M, Grosch W (2000) Potent odorants of raw Arabica coffee. Their changes during roasting. J Agric Food Chem 48(3):868–872

    Article  CAS  Google Scholar 

  35. Daglia M, Papetti A, Aceti C, Sordelli B, Spini V, Gazzani G (2007) Isolation and determination of α-dicarbonyl compounds by RP-HPLC-DAD in green and roasted coffee. J Agric Food Chem 55(22):8877–8882

    Article  CAS  Google Scholar 

  36. Ludwig E, Lipke U, Raczek U, Jäger A (2000) Investigations of peptides and proteases in green coffee beans. Eur Food Res Technol 211(2):111–116

    Article  CAS  Google Scholar 

  37. De Bruyn F, Zhang SJ, Pothakos V, Torres J, Lambot C, Moroni AV, Callanan M, Sybesma W, Weckx S, De Vuyst L (2017) Exploring the impacts of postharvest processing on the microbiota and metabolite profiles during green coffee bean production. Appl Environ Microbiol 83(1):e02398-e12316

    Article  Google Scholar 

  38. Yu A-N, Zhang A-D (2010) The effect of pH on the formation of aroma compounds produced by heating a model system containing l-ascorbic acid with l-threonine/l-serine. Food Chem 119(1):214–219

    Article  CAS  Google Scholar 

  39. Liu C, Yang Q, Linforth R, Fisk ID, Yang N (2019) Modifying Robusta coffee aroma by green bean chemical pre-treatment. Food Chem 272:251–257

    Article  CAS  Google Scholar 

  40. Blank I, Sen A, Grosch W (1991) Aroma impact compounds of Arabica and Robusta coffee. Qualitative and quantitative investigations. In: 14th International Scientific Colloquium on coffee, San Francisco, 1991. ASIC, Paris, pp 117–129

  41. Sunarharum WB, Williams DJ, Smyth HE (2014) Complexity of coffee flavor: A compositional and sensory perspective. Food Res Int 62:315–325

    Article  CAS  Google Scholar 

  42. Flament I, Gautschi F, Winter M, Willhalm B, Stoll M (1968) Les composants furanniques de l’arome de café: quelques aspects chimiques et spectroscopiques, 1968. Association Scientifique Internationale du Café, París (Francia)

    Google Scholar 

  43. Pereira GVdM, Soccol VT, Pandey A, Medeiros ABP, Lara JMRA, Gollo AL, Soccol CR (2014) Isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process. Int J Food Microbiol 188:60–66

    Article  Google Scholar 

  44. Febrianto NA, Yang TA, Wan Abdullah WA (2016) Cocoa-like flavor compound development of Rambutan seed fat as the effect of fermentation and roasting. Int Food Res J 23(5):2166–2174

    CAS  Google Scholar 

  45. Mottram R (2005) The LRI and odour database. https://www.odour.org.uk/lriindex.html. Accessed 23 Jan 2020

Download references

Acknowledgements

The authors gratefully acknowledge Mr. Benji Salim Ang, Director of The Q Coffee Trading, for assisting in preparing the coffee beans and arranging the sensory evaluation tests.

Funding

Not applicable.

Author information

Authors and Affiliations

Authors

Contributions

HE designed and conducted experiments, perform the data analysis and wrote the manuscript. JZ and DF supervised the experiments progress, interpreted the scientific values of the obtained data and proofread the manuscript. JC supervised work. All authors read and approved the manuscript.

Corresponding author

Correspondence to Jian Zhao.

Ethics declarations

Conflict of interest

The authors confirm that they have no conflicts of interest with respect to the work described in this manuscript.

Ethical approval

This experiment was conducted at the University of New South Wales Sydney with human ethics approval by the HREAP Executives (HC Number HC190689).

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Elhalis, H., Cox, J., Frank, D. et al. The role of wet fermentation in enhancing coffee flavor, aroma and sensory quality. Eur Food Res Technol 247, 485–498 (2021). https://doi.org/10.1007/s00217-020-03641-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-020-03641-6

Keywords

Navigation