Vegan food claims to be free of all animal material. This must be verified by the food control authority and the production control laboratory. The method must be sensitive and able to detect animal material after processing such as pasteurization and storage. We have, therefore, developed a multiplex qPCR to simultaneously detect animal, fish and plant DNA in food samples. The system is cost-efficient and exhibited a high sensitivity. The specificity tests confirmed that all relevant species are detected. The plant-specific system served as a DNA isolation control and was used to generate valid results. A large selection of market samples had been analyzed and showed the expected results and the robustness of this analytical tool in routine analysis.
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Pegels N, González I, Fernández S, García T, Martín R (2012) Sensitive detection of porcine DNA in processed animal proteins using a TaqMan real-time PCR assay. J Food Addit Contam Part A 29(9):1402–1412
Lubis H, Salihah NT, Norizan NA, Hossain MM, Ahmed MU (2018) Fast and sensitive real-time PCR-based detection of porcine DNA in food samples by using Evagreen dye. Food Sci Technol Res 24(5):803–810
Dalmassoa A, Fontanellab E, Piattib P, Civeraa T, Rosatic S, Botteroa MT (2004) A multiplex PCR assay for the identification of animal species in feedstuffs. Mol Cell Probes 18:81–87
Ilhak IO, Arslan A (2007) Identification of meat species by polymerase chain reaction (PCR) technique. Turk J Vet Anim Sci 31(3):159–163
Spychaj A, Szalata M, Słomski R, Pospiech E (2016) Identification of bovine, pig and duck meat species in mixtures and in meat products on the basis of the mtDNA cytochrome oxidase Subunit I (COI) gene sequence. Pol J Food Nutr Sci 66(1):31–36
Hazra T, Sharma V, Sharma R, Arora S (2017) A species specific simplex polymerase chain reaction-based approach for detection of goat tallow in heat clarified milk fat (ghee). Int J Food Prop 20(sup1):S69–S75
Bottero MT, Dalmasso IA, Nucera D, Turi RM, Rosati S, Squadrone S, Goria M, Civera T (2003) Development of a PCR assay for the detection of animal tissues in ruminant feeds. J Food Prot 66(12):2307–2312
Fumière O, Marien A, Berben G (2016) Validation study of a real-time PCR method developed by TNO Triskelion bv for the detection of ruminant DNA in feedingstuffs. Centre Wallon de Recherches agronomiques Gembloux (Final version 1.0, 19 p. + annexes. ISBN 978–2- 87286–095–1)
Chen X, Lu L, Xiong X, Xiong X, Liu Y (2020) Development of a real-time PCR assay for the identification and quantification of bovine ingredient in processed meat products. Sci Rep. https://doi.org/10.1038/s41598-020-59010-6
Debode F, Janssen E, Marien A, Berben G (2012) DNA detection by conventional and real-time PCR after extraction from vegetable oils. J Am Oil Chem Soc 89:1249–1257
WHO (2015) Q&A on the carcinogenicity of the consumption of red meat and processed meat. WHO (26 October 2015, Q&A)
Kanakubo K, Fascetti AJ, Larsen JA (2016) Determination of mammalian deoxyribonucleic acid (DNA) in commercial vegetarian and vegan diets for dogs and cats. J Anim Physiol Anim Nutr 101(1):70–74
Fossati LA, Larsen JA, Villaverde C, Fascetti AJ (2018) Determination of mammalian DNA in commercial canine diets with uncommon and limited ingredients. Vet Med Sci 5(1):30–38
Mi X, Yang J, Cao L, Wei X, Zhu Y, Li Q, Liu X, He X, Liao Q, Yan Z (2015) Potential DNA markers as a rapid tracing tool for animal adulterants in vegetarian food. Food Res Int 76(4):926–931
Druml B, Kaltenbrunner M, Hochegger R, Cichna-Markl M (2016) A novel reference real-time PCR assay for the relative quantification of (game) meat species in raw and heat-processed food. Food Control 70:392–400
Tetzlaff C, Mäde D (2017) Development of a real-time PCR system for the detection of the potential allergen fish in food. Eur Food Res Technol 243:849–857
Eugster A, Murmann P, Känzig A, Breitenmoser A (2017) Specific but nevertheless simple real-time PCR method for the detection of irradiated food shown detailed at the example of garlic (Allium sativum). Eur Food Res Technol 244:819–825
Morf NV, Wood KL, Köppel R, Felderer N, Daniels M, Tenger B, Kratzer A (2013) A multiplex PCR method to identify bushmeat species in wildlife; forensics. Forensic Sci Int Genet Suppl Ser 4(1):e202–e203
Köppel R, Zimmerli F, Breitenmoser A (2009) Heptaplex real-time PCR for the identification and quantification of DNA from beef, pork, chicken, turkey, horsemeat, sheep (mutton) and goat. Eur Food Res Technol 230:125–133
Köppel R, Zimmerli F (2010) Proposal for a performance factor to characterize real-time PCR systems, evaluate premixed DNA-polymerases and assign minimal performance criteria for individual quantitative PCR runs. Eur Food Res Technol 231:727–732
We thank the cantonal laboratory of Zürich for providing the resources for this work.
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The authors declare that they have no conflict of interest.
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Köppel, R., Lederman, R., van Velsen, F. et al. Detection of animal DNA in vegan food by multiplex qPCR system. Eur Food Res Technol (2020). https://doi.org/10.1007/s00217-020-03608-7
- Multiplex qPCR
- Animal DNA
- Plant DNA
- Fish DNA