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Evaluation of physicochemical properties on meringue prepared from phospholipase A2 enzyme-hydrolyzed liquid egg albumen


This research aimed to investigate the effects of various concentrations (0.1; 0.2 and 0.3 v/v %) of phospholipase A2 (PLA) enzyme-modified liquid egg albumen (LEA) on color, water activity, specific volume, textural profile analysis, and sensorial characteristics of cooked meringues for 90 days storage period at room temperature. Also, the rheological creep-recovery changes, specific density, color, and physico-functional properties of meringue batters were evaluated. The meringue batter density in the control group (0.47 ± 0.01) decreased significantly (p < 0.05) after PLA hydrolysis, while better specific gravity values were obtained in 0.3% enzyme concentration (0.36 ± 0.01). The use of the enzyme-hydrolyzed LEA also significantly (p < 0.05) decreased water activity with control, 0.1, 0.2 and 0.3% of PLA (0.447 ± 0.01; 0.429 ± 0.01; 0.429 ± 0.01, and 0.420 ± 0.01), respectively. The batter densities of meringues prepared by PLA enzyme decreased with increasing the enzyme concentration, while as enzyme concentration increased, the meringues lost its elasticity. The increase in enzyme concentration leads to a significant increase in meringue’s specific volume values. Sensory analysis of meringues showed that the lowest scores obtained by the control group, and the enzyme hydrolyzed with 0.2% were acceptable. The PLA modification provided the storage stability of meringues in hardness values. This study highlights the use of the PLA enzyme-modified LEA in meringue production results in the development of functional properties and storage stability.

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  1. Alavi F, Emam-Djomeh Z, Chen L (2020) Acid-induced gelation of thermal co-aggregates from egg white and hempseed protein: impact of microbial transglutaminase on mechanical and microstructural properties of gels. Food Hydrocoll.

    Article  Google Scholar 

  2. Alavi F, Emam-Djomeh Z, Mohammadian M, Salami M, Moosavi-Movahedi AA (2020) Physico-chemical and foaming properties of nanofibrillated egg white protein and its functionality in meringue batter. Food Hydrocoll 101:105554.

    Article  CAS  Google Scholar 

  3. Cervenka L, Brozkova I, Vytrasova J (2006) Effects of the principal ingredients of biscuits upon water activity. J Food Nutr Res 45:39–43.

    Article  Google Scholar 

  4. Chang YI, Chen TC, Lee GH, Chang KS (1999) Rheological, surface and colorbvietric properties of egg albumen gel affected by pH. Int J Food Prop 2:101–111.

    Article  CAS  Google Scholar 

  5. Chauhan VS, Sharma A (2003) Studies on organoleptic properties of food products from fresh egg and egg powder through principal component analysis. Nahrung/Food 47:102–105

    Article  Google Scholar 

  6. Culetu A, Stoica-Guzun A, Duta DE (2020) Impact of fat types on the rheological and textural properties of gluten-free oat dough and cookie. Int J Food Sci Technol.

    Article  Google Scholar 

  7. Damian JJ, Huo S, Serventi L (2018) Phytochemical content and emulsifying ability of pulses cooking water. Eur Food Res Technol.

    Article  Google Scholar 

  8. De Maria L, Vind J, Oxenboll KM, Svendsen A, Patkar S (2007) Phospholipases and their industrial applications. Appl Microbiol Biotechnol 74:290–300.

    Article  PubMed  CAS  Google Scholar 

  9. Foegeding EA, Luck PJ, Davis JP (2006) Factors determining the physical properties of protein foams. Food Hydrocoll 20:284–292.

    Article  CAS  Google Scholar 

  10. Guadarrama-Lezama AY, Carrillo-Navas H, Pérez-Alonso C, Vernon-Carter EJ, Alvarez-Ramirez J (2016) Thermal and rheological properties of sponge cake batters and texture and microstructural characteristics of sponge cake made with native corn starch in partial or total replacement of wheat flour. LWT Food Sci Technol 70:46–54.

    Article  CAS  Google Scholar 

  11. Hui YH (2006) Bakery products science and technology. Blackwell Publishing Professional, USA

    Book  Google Scholar 

  12. Kim M-R, Shim J-Y, Park K-H, Imm B-Y, Oh S (2009) Optimization of the enzymatic modification of egg yolk by phospholipase a2 to improve its functionality for mayonnaise production. LWT Food Sci and Techn 42:250–255.

    Article  CAS  Google Scholar 

  13. Lechevalier V, Jeantet R, Arhaliass A, Legrand J, Nau F (2007) Egg white drying: influence of industrial processing steps on protein structure and functionalities. J of Food Eng 83:404–413.

    Article  Google Scholar 

  14. Licciardello F, Frisullo P, Laverse J, Muratore G, Del Nobile MA (2012) Effect of sugar, citric acid and egg white type on the microstructural and mechanical properties of meringues. J Food Eng 108:453–462.

    Article  CAS  Google Scholar 

  15. Lomakina K, Mikova K (2006) A study of the factors affecting the foaming properties of egg white–a review. Czech J Food Sci 24:110–118.

    Article  CAS  Google Scholar 

  16. Marcet I, Collado S, Paredes B, Díaz M (2016) Rheological and textural properties in a bakery product as a function of the proportions of the egg yolk fractions: discussion and modelling. Food Hydrocoll 54:119–129.

    Article  CAS  Google Scholar 

  17. Matos ME, Sanz T, Rosell CM (2014) Establishing the function of proteins on the rheological and quality properties of rice based gluten free muffins. Food Hydrocoll 35:150–158.

    Article  CAS  Google Scholar 

  18. Mizu T, Nagao K (2010) Thermal conduction in egg albumen foam (meringue) during heating. J Jpn Soc Food Sci 57:20–25

    Article  Google Scholar 

  19. Nastaj M, Sołowiej BG, Terpiłowski K, Mleko S (2020) Effect of erythritol on physicochemical properties of reformulated high protein meringues obtained from whey protein isolate. Int Dairy J.

    Article  Google Scholar 

  20. O'Charoen S, Hayakawa S, Matsumoto Y, Ogawa M (2014) Effect of D-psicose used as sucrose replacer on the characteristics of meringue. J Food Sci 79:E2463–E2469.

    Article  PubMed  CAS  Google Scholar 

  21. Primacella M, Wang T, Acevedo NC (2019) Characterization of mayonnaise properties prepared using frozen-thawed egg yolk treated with hydrolyzed egg yolk proteins as anti-gelator. Food Hydrocoll 96:529–536.

    Article  CAS  Google Scholar 

  22. Punidadas P, McKellar RC (1999) Selected physical properties of liquid egg products at pasteurization temperatures. J Food Process Preserv 23:153–168.

    Article  Google Scholar 

  23. Raikos V, Campbell L, Euston SR (2007) Effects of sucrose and sodium chloride on foaming properties of egg white proteins. Food Res Int 40:347–355.

    Article  CAS  Google Scholar 

  24. Sadahira MS, Rodrigues MI, Akhtar M, Murray BS, Netto FM (2016) Effect of egg white protein-pectin electrostatic interactions in a high sugar content system on foaming and foam rheological properties. Food Hydrocoll 58:1–10.

    Article  CAS  Google Scholar 

  25. Sadahira MS, Rodrigues MI, Akhtar M, Murray BS, Netto FM (2018) Influence of pH on foaming and rheological properties of aerated high sugar system with egg white protein and hydroxypropylmethylcellulose. Lwt-Food Sci Technol 89:350–357.

    Article  CAS  Google Scholar 

  26. SAS (2003) Institute Inc. SAS user guide, Version 9.1.3. Statistical Analysis Systems Institute. Cary, NC, USA

  27. Singh A, Geveke DJ, Jones DR, Tilman ED (2019) Can acceptable quality angel food cakes be made using pasteurized shell eggs? The effects of mixing factors on functional properties of angel food cakes. Food Sci Nutr.

    Article  PubMed  PubMed Central  Google Scholar 

  28. Starmer D, Coate K, Terry P (2018) The effects of creating a vegan alternative to hard meringues by substituting aquafaba for egg whites. J Acad Nutr Diet 118:A51.

    Article  Google Scholar 

  29. Stevenson P, Mantle MD, Hicks JM (2007) NMRI studies of the free drainage of egg white and meringue mixture froths. Food Hydrocolloids 21:221–229.

    Article  CAS  Google Scholar 

  30. Vaclavik VA, Christian EW (2014) Sugars, Sweeteners, and Confections. In: Vaclavik VA, Christian EW (eds) Essentials of Food Science Food Science Text Series. Springer, New York, p 306

    Chapter  Google Scholar 

  31. Wills D (1998) Water activity and its ımportance in making candy. Manuf Confect 78:71–74

    Google Scholar 

  32. Wouters AGB, Rombouts I, Fierens E, Brijs K, Delcour JA (2018) Enzymatically hydrolyzed wheat gluten as a foaming agent in food: incorporation in a meringue recipe as a proof-of-concept. J Food Sci 83:2119–2126.

    Article  PubMed  CAS  Google Scholar 

  33. Wouters AGB, Rombouts I, Fierens E, Brijs K, Delcour JA (2018) Enzymatically hydrolyzed wheat gluten as a foaming agent in food: incorporation in a meringue recipe as a proof-of-concept. J Food Sci.

    Article  PubMed  Google Scholar 

  34. Yuceer M (2020) Structural and rheological characterization of liquid egg white modified with phospholipase A2 enzyme. J Food Process Preserv.

    Article  Google Scholar 

  35. Yüceer M, Asik H (2020) Texture, Rheology, Storage Stability and Sensory Evaluation of Meringue’s Prepared from Lipase Enzyme-Modified Liquid Egg White. J Food Process Preserv.

    Article  Google Scholar 

  36. Yuceer M., Caner C., New Method of Production Meringues Using Enzyme Modified Liquid Egg. Turkey Patent (2018).

  37. Yüceer M, Caner C (2014) Antimicrobial lysozyme-chitosan coatings affect functional properties and shelf life of chicken eggs during storage. J Sci Food Agric 94:153–162.

    Article  PubMed  CAS  Google Scholar 

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This study supported by TUBİTAK, the Scientific and Technical Council of Turkey (grant number 214O376). The authors gratefully acknowledge Prof. Dr. Cengiz Caner for contribution to the study.

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Correspondence to Muhammed Yüceer.

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Yüceer, M. Evaluation of physicochemical properties on meringue prepared from phospholipase A2 enzyme-hydrolyzed liquid egg albumen. Eur Food Res Technol 246, 1847–1856 (2020).

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  • Liquid egg white
  • Phospholipase A2
  • Meringue
  • Creep-recovery rheology
  • Storage stability
  • Sensorial and textural characterization