The present study investigated how different storage conditions affect antioxidant levels in three scab-resistant and powdery mildew-tolerant apple cultivars. The contents of carotenoids, along with free and glycosylated phenolic acids, were quantified in harvested apples and apples stored in boxes with controlled atmospheric conditions (1.2% O2 and 2.2% CO2) and temperature (1 °C), boxes with regulated temperature (1 °C) and storage rooms with a temperature between 1 and 4 °C. All three cultivars demonstrated a continuous decrease in total carotenoid content (sum of ß-carotene, lutein, neoxanthin, violaxanthin, zeaxanthin and antheraxanthin) during the storage period. However, apples stored under controlled atmospheric conditions showed significantly higher carotenoid levels than apples stored under other approaches over 3 and 5 months of storage. Although the storage approach clearly affected carotenoid levels across the three tested cultivars, the ratio between the levels of individual carotenoids was neither affected by storage approach nor cultivar. Free phenolic acid contents were found to be cultivar specific; whereas, all three cultivars demonstrated similar glycosylated phenolic acid contents. Chlorogenic acid was the most common free phenolic acid in all three cultivars, while protocatechuic and caffeic acid were the dominant glycosylated phenolic acids identified from the cultivars. Interestingly, stored apples showed higher free phenolic acid levels than harvested apples; while, both harvested and stored apples showed similar concentrations of glycosylated phenolic acids. As such, none of the three tested storage conditions had a pronounced effect on either group of phenolic acids (total content or concentrations of individual compounds).
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Gutierrez-Grijalva EP, Ambriz-Pere DL, Leyva-Lopez N, Castillo-Lopez RI, Heredia JB (2016) Review: dietary phenolic compounds, health benefits and bioaccessibility. Arch Latinoam Nutr 66(2):87–100
Yahia EM, Barrera A (2010) Antioxidant capacity and correlation with phenolic compounds and carotenoids in 40 horticultural commodities. In: Erkan M, Aksoy U (eds) Vi international postharvest symposium, vol 877. Acta Horticulturae, pp 1215–1220
Sun J, Chu YF, Wu XZ, Liu RH (2002) Antioxidant and anti proliferative activities of common fruits. J Agric Food Chem 50(25):7449–7454. https://doi.org/10.1021/jf0207530
Wolfe KL, Liu RH (2003) Apple peels as a value-added food ingredient. J Agric Food Chem 51(6):1676–1683. https://doi.org/10.1021/jf025916z
Lee J, Chan BLS, Mitchell AE (2017) Identification/quantification of free and bound phenolic acids in peel and pulp of apples (Malus domestica) using high resolution mass spectrometry (HRMS). Food Chem 215:301–310. https://doi.org/10.1016/j.foodchem.2016.07.166
Boyer J, Liu RH (2004) Apple phytochemicals and their health benefits. Nutr J. https://doi.org/10.1186/1475-2891-3-5
Galler M, Mackinne G (1965) Carotenoids of certain fruits (apple, pear, cherry, strawberry). J Food Sci 30(3):393
Delgado-Pelayo R, Gallardo-Guerrero L, Hornero-Mendez D (2014) Chlorophyll and carotenoid pigments in the peel and flesh of commercial apple fruit varieties. Food Res Int 65:272–281. https://doi.org/10.1016/j.foodres.2014.03.025
Felicetti DA, Schrader LE (2009) Changes in pigment concentrations associated with sunburn browning of five apple cultivars. I. Chlorophylls and carotenoids. Plant Sci 176(1):78–83. https://doi.org/10.1016/j.plantsci.2008.09.013
Jakobek L, Barron AR (2016) Ancient apple varieties from Croatia as a source of bioactive polyphenolic compounds. J Food Compos Anal 45:9–15. https://doi.org/10.1016/j.jfca.2015.09.007
Gross J, Zachariae A, Lenz F, Eckhardt G (1978) Carotenoid changes in peel of golden delicious apple during ripening and storage. Z Pflanzenphysiol 89(4):321–332
Lee KW, Kim YJ, Kim DO, Lee HJ, Lee CY (2003) Major phenolics in apple and their contribution to the total antioxidant capacity. J Agric Food Chem 51(22):6516–6520. https://doi.org/10.1021/jf034475w
Kumar P, Sethi S, Sharma RR, Singh S, Saha S, Sharma VK, Verma MK, Sharma SK (2018) Nutritional characterization of apple as a function of genotype. J Food Sci Technol-Mysore 55(7):2729–2738. https://doi.org/10.1007/s13197-018-3195-x
Stracke BA, Rufer CE, Watzl B (2010) Polyphenol and carotenoid content of organically and conventionally produced apples (Malus domestica Bork., Elstar Variety) and carrots (Daucus carota L., Narbonne and Nerac Varieties). Ernahr Umsch 57(10):526–531
Yano M, Kato M, Ikoma Y, Kawasaki A, Fukazawa Y, Sugiura M, Matsumoto H, Oohara Y, Nagao A, Ogawa K (2005) Quantitation of carotenoids in raw and processed fruits in Japan. Food Sci Technol Res 11(1):13–18. https://doi.org/10.3136/fstr.11.13
Dias MG, Camoes M, Oliveira L (2009) Carotenoids in traditional Portuguese fruits and vegetables. Food Chem 113(3):808–815. https://doi.org/10.1016/j.foodchem.2008.08.002
Bahukhandi A, Dhyani P, Bhatt ID, Rawal RS (2018) Variation in polyphenolics and antioxidant activity of traditional apple cultivars from West Himalaya, Uttarakhand. Hortic Plant J 4(4):151–157. https://doi.org/10.1016/j.hpj.2018.05.001
Slatnar A, Mikulic-Petkovsek M, Halbwirth H, Stampar F, Stich K, Veberic R (2012) Polyphenol metabolism of developing apple skin of a scab resistant and a susceptible apple cultivar. Trees-Struct Funct 26(1):109–119. https://doi.org/10.1007/s00468-011-0577-3
Mikulic-Petkovsek M, Stampar F, Veberic R (2008) Increased phenolic content in apple leaves infected with the apple scab pathogen. J Plant Pathol 90(1):49–55
Mikulic-Petkovsek M, Stampar F, Veberic R (2009) Accumulation of phenolic compounds in apple in response to infection by the scab pathogen, Venturia inaequalis. Physiol Mol Plant Pathol 74(1):60–67. https://doi.org/10.1016/j.pmpp.2009.09.003
Mikulic-Petkovsek M, Stampar F, Veberic R (2007) Parameters of inner quality of the apple scab resistant and susceptible apple cultivars (Malus domestica Borkh.). Sci Hortic 114(1):37–44. https://doi.org/10.1016/j.scienta.2007.05.004
Manganaris GA, Goulas V, Mellidou I, Drogoudi P (2018) Antioxidant phytochemicals in fresh produce: exploitation of genotype variation and advancements in analytical protocols. Front Chem. https://doi.org/10.3389/fchem.2017.00095
Radenkovs V, Juhnevica-Radenkova K (2017) Effect of storage technology on the chemical composition of apples of the cultivar ‘Auksis’. Zemdirb Agric 104(4):359–368. https://doi.org/10.13080/z-a.2017.104.046
Soleti R, Hilairet G, Mallegol P, Dourguia C, Frifra M, Guillou MC, Gacel A, Guyot S, Pignon P, Basset L, Cadot Y, Renou JP, Orsel M, Andriantsitohaina R (2018) Screening of ordinary commercial varieties of apple fruits under different storage conditions for their potential vascular and metabolic protective properties. Food Funct 9(11):5855–5867. https://doi.org/10.1039/c8fo00967h
Bessemans N, Verboven P, Verlinden BE, Nicolai BM (2016) A novel type of dynamic controlled atmosphere storage based on the respiratory quotient (RQ-DCA). Postharvest Biol Technol 115:91–102. https://doi.org/10.1016/j.postharvbio.2015.12.019
Lee J, Kang IK, Nock JF, Watkins CB (2019) Effects of preharvest and postharvest applications of 1-methylcyclopropene on fruit quality and physiological disorders of ‘fuji’ apples during storage at warm and cold temperatures. HortScience 54(8):1375–1383. https://doi.org/10.21273/hortsci14062-19
Gwanpua SG, Verlinden BE, Hertog M, Nicolai BM, Geeraerd AH (2017) A mechanistic modelling approach to understand 1-MCP inhibition of ethylene action and quality changes during ripening of apples. J Sci Food Agric 97(11):3802–3813. https://doi.org/10.1002/jsfa.8244
Kalinowska M, Bielawska A, Lewandowska-Siwkiewicz H, Priebe W, Lewandowski W (2014) Apples: content of phenolic compounds vs. variety, part of apple and cultivation model, extraction of phenolic compounds, biological properties. Plant Physiol Biochem 84:169–188. https://doi.org/10.1016/j.plaphy.2014.09.006
Gonzalez-Talice J, Yuri JA, del Pozo A (2013) Relations among pigments, color and phenolic concentrations in the peel of two Gala apple strains according to canopy position and light environment. Sci Hortic 151:83–89. https://doi.org/10.1016/j.scienta.2012.12.007
Prochazkova D, Haisel D, Pavlikova D, Szakova J, Wilhelmova N (2014) The impact of increased soil risk elements on carotenoid contents. Cent Eur J Biol 9(7):678–685. https://doi.org/10.2478/s11535-014-0304-3
Eliasova K, Vondrakova Z, Malbeck J, Travnickova A, Pesek B, Vagner M, Cvikrova M (2017) Histological and biochemical response of Norway spruce somatic embryos to UV-B irradiation. Trees-Struct Funct 31(4):1279–1293. https://doi.org/10.1007/s00468-017-1547-1
Alberti A, Zielinski AAF, Couto M, Judacewski P, Mafra LI, Nogueira A (2017) Distribution of phenolic compounds and antioxidant capacity in apples tissues during ripening. J Food Sci Technol-Mysore 54(6):1511–1518. https://doi.org/10.1007/s13197-017-2582-z
Huber GM, Rupasinghe HPV (2009) Phenolic profiles and antioxidant properties of apple skin extracts. J Food Sci 74(9):C693–C700. https://doi.org/10.1111/j.1750-3841.2009.01356.x
Vrhovsek U, Rigo A, Tonon D, Mattivi F (2004) Quantitation of polyphenols in different apple varieties. J Agric Food Chem 52(21):6532–6538. https://doi.org/10.1021/jf049317z
Stanger MC, Steffens CA, Soethe C, Moreira MA, do Amarante CVT (2017) Phenolic content and antioxidant activity during the development of ‘Brookfield’ and ‘Mishima’ apples. J Agric Food Chem 65(17):3453–3459. https://doi.org/10.1021/acs.jafc.6b04695
Jakobek L, Garcia-Villalba R, Tomas-Barberan FA (2013) Polyphenolic characterisation of old local apple varieties from Southeastern European region. J Food Compos Anal 31(2):199–211. https://doi.org/10.1016/j.jfca.2013.05.012
Khanizadeh S, Tsao R, Rekika D, Yang R, Charles MT, Rupasinghe HPV (2008) Polyphenol composition and total antioxidant capacity of selected apple genotypes for processing. J Food Compos Anal 21(5):396–401. https://doi.org/10.1016/j.jfca.2008.03.004
McGhie TK, Hunt M, Barnett LE (2005) Cultivar and growing region determine the antioxidant polyphenolic concentration and composition of apples grown in New Zealand. J Agric Food Chem 53(8):3065–3070. https://doi.org/10.1021/jf047832r
Scalzo J, Politi A, Pellegrini N, Mezzetti B, Battino M (2005) Plant genotype affects total antioxidant capacity and phenolic contents in fruit. Nutrition 21(2):207–213. https://doi.org/10.1016/j.nut.2004.03.025
Tarola AM, Girelli AM, D’Ascenzo F (2019) Bioactive polyphenol profiles and antioxidant activity in italian apples varietiesI. Ital J Food Sci 31(2):243–252
Lata B, Trampczynska A, Paczesna J (2009) Cultivar variation in apple peel and whole fruit phenolic composition. Sci Hortic 121(2):176–181. https://doi.org/10.1016/j.scienta.2009.01.038
Francini A, Sebastiani L (2013) Phenolic compounds in apple (Malus × domestica borkh.): Compounds characterization and stability during postharvest and after processing. Antioxidants 2(3):181–193. https://doi.org/10.3390/antiox2030181
Burton GW (1989) Antioxidant action of carotenoids. J Nutr 119(1):109–111
Dias MG, Camoes M, Oliveira L (2014) Carotenoid stability in fruits, vegetables and working standards—effect of storage temperature and time. Food Chem 156:37–41. https://doi.org/10.1016/j.foodchem.2014.01.050
van der Sluis AA, Dekker M, de Jager A, Jongen WMF (2001) Activity and concentration of polyphenolic antioxidants in apple: effect of cultivar, harvest year, and storage conditions. J Agric Food Chem 49(8):3606–3613. https://doi.org/10.1021/jf001493u
Tarozzi A, Marchesi A, Cantelli-Forti G, Hrelia P (2004) Cold-storage affects antioxidant properties of apples in caco-2 cells. J Nutr 134(5):1105–1109
Carbone K, Giannini B, Picchi V, Lo Scalzo R, Cecchini F (2011) Phenolic composition and free radical scavenging activity of different apple varieties in relation to the cultivar, tissue type and storage. Food Chem 127(2):493–500. https://doi.org/10.1016/j.foodchem.2011.01.030
Burda S, Oleszek W, Lee CY (1990) Phenolic-compounds and their changes in apples during maturation and cold storage. J Agric Food Chem 38(4):945–948. https://doi.org/10.1021/jf00094a006
Napolitano A, Cascone A, Graziani G, Ferracane R, Scalfi L, Di Vaio C, Ritieni A, Fogliano V (2004) Influence of variety and storage on the polyphenol composition of apple flesh. J Agric Food Chem 52(21):6526–6531. https://doi.org/10.1021/jf049822w
Awad MA, de Jager A (2000) Flavonoid and chlorogenic acid concentrations in skin of ‘Jonagold’ and ‘Elstar’ apples during and after regular and ultra low oxygen storage. Postharvest Biol Technol 20(1):15–24. https://doi.org/10.1016/s0925-5214(00)00116-2
Koricanac A, Miletic N, Popovic B, Mitrovic O, Lukic M, Pesakovic M, Tomic J (2020) The effect of ULO and NA storage on changes in the quality of apple fruit (Malus domestica Borkh) during shelf life. Agron Basel 10(1):25. https://doi.org/10.3390/agronomy10010025
This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (project LTC 17034; within CA COST Action CA 15136—Eurocaroten). The authors thank Sees-editing Ltd. for linguistic editing.
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MS/MS chromatograms and product ion spectra of standards of phenolic acids recorded in negative ESI mode. Deuterated internal standards are marked by *. (PUB 148 kb)
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Vondráková, Z., Trávníčková, A., Malbeck, J. et al. The effect of storage conditions on the carotenoid and phenolic acid contents of selected apple cultivars. Eur Food Res Technol 246, 1783–1794 (2020). https://doi.org/10.1007/s00217-020-03532-w
- Free and glycosylated phenolic acids
- Storage conditions