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Protein aggregation mechanism in UHT milk: supramolecular evidences

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Abstract

The protein aggregation mechanism in UHT milk samples stored at ambient temperature for 1, 3 and 5 months was assessed in this study. Three phases of the UHT milks were studied: supernatants, dispersed phase and sediments. The supernatants showed a great variability, suggesting the presence of a dynamic arrangement within the protein system of UHT milk, which moves towards the formation of the sediment. The application of 2D-electrophoresis (AU-PAGENR-SDS-PAGER) and mass spectrometry analyses were carried out to study the main heat-induced supramolecular protein aggregates. These aggregates were found mainly in the supernatant and their composition changed along the storage, as a consequence of the medium chemical changes, which are temperature- and pH-depended, whereas the composition of the dispersed phase and sediment denoted a hierarchical mechanism of assembling.

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Authors and Affiliations

  1. Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122, Foggia, Italy

    M. Rutigliano, G. Spadaccino, A. Di Luccia & B. la Gatta

  2. Dipartimento di Agricoltura Ambiente e Alimenti, Università del Molise, Via F. De Sanctis, 1, Campobasso, Italy

    G. Rusco

  3. Istituto di Scienze dell’Alimentazione, Consiglio Nazionale per la Ricerca, Via Roma, 52, 83100, Avellino, Italy

    G. Picariello

  4. Dipartimento di Medicina Molecolare e Traslazionale, Università degli Studi di Brescia, Viale Europa 11, 25123, Brescia, Italy

    O. Bulgari

  5. Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via della Salute, 2, 80055, Portici, NA, Italy

    R. Gagliardi

  6. Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, Via Università 100, 80055, Portici, NA, Italy

    F. Addeo

Authors
  1. M. Rutigliano
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  2. G. Rusco
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  3. G. Picariello
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  4. O. Bulgari
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  5. G. Spadaccino
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  6. R. Gagliardi
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  7. A. Di Luccia
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  8. F. Addeo
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  9. B. la Gatta
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Correspondence to B. la Gatta.

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Rutigliano, M., Rusco, G., Picariello, G. et al. Protein aggregation mechanism in UHT milk: supramolecular evidences. Eur Food Res Technol 246, 1081–1094 (2020). https://doi.org/10.1007/s00217-020-03474-3

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  • DOI: https://doi.org/10.1007/s00217-020-03474-3

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