Effect of light intensity and water availability on plant growth, essential oil production and composition in Rosmarinus officinalis L.


The effect of light intensity (LI) and water availability (WA) on rosemary (Rosmarinus officinalis L.) plant growth, essential oil (EO) production and composition was investigated by a two-factorial field experiment, where the first factor was LI (100%, 50% or 25% of natural sunlight) and the second factor was WA (irrigation set at 85%, 70% or 55% of field capacity during plant growing). The EO obtained by steam distillation of the dried aerial part of the plant was analysed by GC/MS. Reduction of LI from 100 to 25% of natural sunlight markedly lowered plant biomass production, whereas reduction of WA from 85 to 55% had a smaller lowering effect on plant growth. High shading (25% of LI) markedly reduced EO yield on a plant basis (− 43%), whereas intermediate shading (50% of LI) increased EO yield as % content of the fresh biomass (+ 29%) when compared to full solar radiation. WA markedly influenced EO yield, as expressed on a plant basis, but only in plants exposed to 100% LI. Moreover, changes in LI and WA seemed to have an opposite effect on the relative abundance of EO constituents that are formed through the activity of two groups of enzymes, pinene synthases (α- and β-pinene, camphene and myrcene) and, respectively, bornyl diphosphate synthases (borneol, camphor and bornyl acetate). Accurate management of light conditions and water availability, in greenhouse as well as open field conditions, may allow to optimize rosemary EO yield and modulate EO profile in view of different potential uses.

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  1. 1.

    Sasikumar B (2012) In: Peter KV (ed) Handbook of herbs and spices, vol 1, 2nd edn. Woodhead Publishing Limited, Cambridge

    Google Scholar 

  2. 2.

    Brud WS (2009) In: Başer KHC, Buchbauer G (eds) Handbook of essential oils: science, technology, and applications. CRC Press, Boca Raton

    Google Scholar 

  3. 3.

    Lubbe A, Verpoorte R (2011) Cultivation of medicinal and aromatic plants for specialty industrial materials. Ind Crops Prod 34:785–801. https://doi.org/10.1016/j.indcrop.2011.01.019

    CAS  Article  Google Scholar 

  4. 4.

    del Pilar Sánchez-Camargo A, Herrero M (2017) Rosemary (Rosmarinus officinalis) as a functional ingredient: recent scientific evidence. Curr Opin Food Sci 14:13–19. https://doi.org/10.1016/j.cofs.2016.12.003

    Article  Google Scholar 

  5. 5.

    Ribeiro-Santos R, Andrade M, de Melo NR, Sanches-Silva A (2017) Use of essential oils in active food packaging: recent advances and future trends. Trends Food Sci Technol 61:132–140. https://doi.org/10.1016/j.tifs.2016.11.021

    CAS  Article  Google Scholar 

  6. 6.

    Cervelli C, Fadelli PG, Tallone A (2001) Growth of Oreopanax capitatus and Cocculus laurifolius in different protected environments. Acta Hortic 559:91–96

    Article  Google Scholar 

  7. 7.

    Conover C (1990) In: Novak J, Rudnicki RM (eds) Postharvest handling and storage of cut flowers, florist’s green and potted plants. Timber Press, Portland

    Google Scholar 

  8. 8.

    Gratani L, Covone F, Larcher W (2006) Leaf plasticity in response to light of three evergreen species of the Mediterranean maquis. Trees 20:549–558

    Article  Google Scholar 

  9. 9.

    Gregory PJ, Palta JA, Batts GR (1997) Root systems and root: mass ratio—carbon allocation under current and projected atmospheric conditions in arable crops. Plant Soil 187:221–228

    Article  Google Scholar 

  10. 10.

    Kozlowski TT, Pallardy SG (1997) Growth control in woody plants. Academic Press, San Diego

    Google Scholar 

  11. 11.

    Mielke MS, Schaffer B (2010) Photosynthetic and growth responses of Eugenia uniflora L. seedlings to soil flooding and light intensity. Environ Exp Bot 68:113–121. https://doi.org/10.1016/j.envexpbot.2009.11.007

    CAS  Article  Google Scholar 

  12. 12.

    Osakabe Y, Osakabe K, Shinozaki K, Tran L-SP (2014) Response of plants to water stress. Front Plant Sci 5:86

    Article  Google Scholar 

  13. 13.

    Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJC (2008) Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour Fragr J 23:213–226. https://doi.org/10.1002/ffj.1875

    CAS  Article  Google Scholar 

  14. 14.

    Franz C, Novak J (2009) In: Başer KHC, Buchbauer G (eds) Handbook of essential oils: science, technology, and applications. CRC Press, Boca Raton

    Google Scholar 

  15. 15.

    Loreto F, Ciccioli P, Cecinato A et al (1996) Influence of environmental factors and air composition on the emission of [alpha]-pinene from quercus ilex leaves. Plant Physiol 110:267–275

    CAS  Article  Google Scholar 

  16. 16.

    Li Y, Craker LE, Potter T (1996) Effect of light level on the essential oil production of sage (Salvia officinalis) and thyme (Thymus vulgaris). Acta Hortic 426:419–426

    CAS  Article  Google Scholar 

  17. 17.

    Tibaldi G, Fontana E, Nicola S (2011) Growing conditions and postharvest management can affect the essential oil of Origanum vulgare L. ssp. hirtum (Link) Ietswaart. Ind Crops Prod 34:1516–1522. https://doi.org/10.1016/j.indcrop.2011.05.008

    CAS  Article  Google Scholar 

  18. 18.

    Chang X, Alderson PG, Wright CJ (2008) Solar irradiance level alters the growth of basil (Ocimum basilicum L.) and its content of volatile oils. Environ Exp Bot 63:216–223. https://doi.org/10.1016/j.envexpbot.2007.10.017

    CAS  Article  Google Scholar 

  19. 19.

    Rios-Estepa R, Turner GW, Lee JM et al (2008) A systems biology approach identifies the biochemical mechanisms regulating monoterpenoid essential oil composition in peppermint. Proc Natl Acad Sci 105:2818–2823. https://doi.org/10.1073/pnas.0712314105

    Article  PubMed  Google Scholar 

  20. 20.

    Mousavinik SM, Asgharipour MR, Sardashti S (2016) Manure and light intensity affect growth characteristics and essential oil of peppermint (Mentha piperita L.). J Essent Oil Bear Plants 19:2029–2036. https://doi.org/10.1080/0972060X.2016.1242435

    CAS  Article  Google Scholar 

  21. 21.

    Degani AV, Dudai N, Bechar A, Vaknin Y (2016) Shade effects on leaf production and essential oil content and composition of the novel herb Eucalyptus citriodora hook. J Essent Oil Bear Plants 19:410–420. https://doi.org/10.1080/0972060X.2014.890080

    CAS  Article  Google Scholar 

  22. 22.

    Selmar D, Kleinwächter M (2013) Influencing the product quality by deliberately applying drought stress during the cultivation of medicinal plants. Ind Crops Prod 42:558–566. https://doi.org/10.1016/j.indcrop.2012.06.020

    CAS  Article  Google Scholar 

  23. 23.

    Chrysargyris A, Laoutari S, Litskas VD et al (2016) Effects of water stress on lavender and sage biomass production, essential oil composition and biocidal properties against Tetranychus urticae (Koch). Sci Hortic 213:96–103. https://doi.org/10.1016/j.scienta.2016.10.024

    CAS  Article  Google Scholar 

  24. 24.

    Delfine S, Loreto F, Pinelli P et al (2005) Isoprenoids content and photosynthetic limitations in rosemary and spearmint plants under water stress. Agric Ecosyst Environ 106:243–252. https://doi.org/10.1016/j.agee.2004.10.012

    CAS  Article  Google Scholar 

  25. 25.

    Li G, Cervelli C, Ruffoni B et al (2016) Volatile diversity in wild populations of rosemary (Rosmarinus officinalis L.) from the Tyrrhenian Sea vicinity cultivated under homogeneous environmental conditions. Ind Crops Prod 84:381–390. https://doi.org/10.1016/j.indcrop.2016.02.029

    CAS  Article  Google Scholar 

  26. 26.

    Thakur M, Bhattacharya S, Khosla PK, Puri S (2018) Improving production of plant secondary metabolites through biotic and abiotic elicitation. J Appl Res Med Aromat Plants. https://doi.org/10.1016/j.jarmap.2018.11.004

    Article  Google Scholar 

  27. 27.

    Cingolani E (1973) Italian pharmacopoeia—8th edition. Farmaco Prat 28(11):559–584

    CAS  PubMed  Google Scholar 

  28. 28.

    NIST (National Institute of Standards and Technology), NIST chemistry WebBook, NIST standard reference database number 69, 2018, available online at: http://webbook.nist.gov/chemistry. Accessed on July 2019

  29. 29.

    Hammer O, Harper D, Ryan P (2012) PAST: paleontological statistics software package for education and data analysis. Paleontol Electron 4(art. 4):9

    Google Scholar 

  30. 30.

    Givnish T (1988) Adaptation to sun and shade: a whole-plant perspective. Funct Plant Biol 15:63. https://doi.org/10.1071/PP9880063

    Article  Google Scholar 

  31. 31.

    Kumar R, Sharma S, Pathania V (2013) Effect of shading and plant density on growth, yield and oil composition of clary sage (Salvia sclarea L.) in north western Himalaya. J Essent Oil Res 25:23–32. https://doi.org/10.1080/10412905.2012.742467

    CAS  Article  Google Scholar 

  32. 32.

    Zervoudakis G, Salahas G, Kaspiris G, Konstantinpoulou E (2012) Influence of light intensity on growth and physiological characteristics of common sage (Salvia officinalis L.). Braz Arch Biol Technol 55:88–95. https://doi.org/10.1590/S1516-89132012000100011

    CAS  Article  Google Scholar 

  33. 33.

    Sánchez-Blanco JM, Ferrández T, Navarro A, Bañon S, José Alarcón J (2004) Effects of irrigation and air humidity preconditioning on water relations, growth and survival of Rosmarinus officinalis plants during and after transplanting. J Plant Physiol 161:1133–1142. https://doi.org/10.1016/j.jplph.2004.01.011

    CAS  Article  PubMed  Google Scholar 

  34. 34.

    Flamini G, Cioni PL, Morelli I et al (2002) Main agronomic—productive characteristics of two ecotypes of Rosmarinus officinalis L. and chemical composition of their essential oils. J Agric Food Chem 50:3512–3517. https://doi.org/10.1021/jf011138j

    CAS  Article  PubMed  Google Scholar 

  35. 35.

    Sell C (2009) In: Başer KHC, Buchbauer G (eds) Handbook of essential oils: science, technology, and applications. CRC Press, Boca Raton

    Google Scholar 

  36. 36.

    Wise ML, Savage TJ, Katahira E, Croteau R (1998) Monoterpene synthases from common sage (Salvia officinalis) cDNA isolation, characterization, and functional expression of (+)-sabinene synthase, 1, 8-cineole synthase, and (+)-bornyl diphosphate synthase. J Biol Chem 273:14891–14899. https://doi.org/10.1074/jbc.273.24.14891

    CAS  Article  PubMed  Google Scholar 

  37. 37.

    Radwan A, Kleinwächter M, Selmar D (2017) Impact of drought stress on specialised metabolism: biosynthesis and the expression of monoterpene synthases in sage (Salvia officinalis). Phytochemistry 141:20–26. https://doi.org/10.1016/j.phytochem.2017.05.005

    CAS  Article  PubMed  Google Scholar 

  38. 38.

    Kamath A, Asha MR, Ravi R et al (2001) Comparative study of odour and GC-olfactometric profiles of selected essential oils. Flavour Fragr J 16:401–407. https://doi.org/10.1002/ffj.1020

    CAS  Article  Google Scholar 

  39. 39.

    Zaouali Y, Bouzaine T, Boussaid M (2010) Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food Chem Toxicol 48:3144–3152. https://doi.org/10.1016/j.fct.2010.08.010

    CAS  Article  PubMed  Google Scholar 

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The study was carried out with the financial support of the Ministry of Agriculture, Food and Forestry Policies and Tourism, Italy, within the project “Implementation of the FAO International Treaty on Plant Genetic Resources for Food and Agriculture”.

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Raffo, A., Mozzanini, E., Ferrari Nicoli, S. et al. Effect of light intensity and water availability on plant growth, essential oil production and composition in Rosmarinus officinalis L.. Eur Food Res Technol 246, 167–177 (2020). https://doi.org/10.1007/s00217-019-03396-9

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  • Rosemary
  • Terpenoids
  • Monoterpene synthases
  • Solar radiation
  • Aroma
  • Irrigation