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Biofilm and dairy fouling detection in flexible tubing using low-field NMR

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Abstract

Biofilm and dairy fouling formation is difficult to study non-invasively in closed systems such as flexible tubing. Nuclear magnetic resonance (NMR) is considered as a promising non-invasive detection method for different deposits. This study aimed to investigate three deposit models: (1) Paenibacillus polymyxa biofilm, (2) dairy biofouling, and (3) dairy fouling, in different experimental set-ups by low-field 1H NMR. The transverse relaxation time T2 of each component in samples of experimental set-ups was studied. The results show that low-field NMR offers the possibility of qualitatively measuring the selected deposit models and differentiating non-destructively between the tubing and each deposit. The samples in each experimental set-up showed characteristic relaxation behavior of flexible tubing, deposit, and liquid. For the P. polymyxa biofilm, longer relaxation times T2 of about 100 ms were detected, while relaxation times T2 for dairy biofouling and dairy fouling were in range of 50–60 ms. It was thus concluded that low-field NMR has a great potential for non-invasive detection of deposit formation at practical industrial scales.

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Author notes

  1. Olga Fysun

    Present address: Robert Bosch GmbH, Reutlingen, Germany

  2. Olga Fysun and Theresa Anzmann have contributed equally to this work.

Authors and Affiliations

  1. TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany

    Olga Fysun & Horst-Christian Langowski

  2. Robert Bosch Packaging Technology GmbH, Waiblingen, Germany

    Olga Fysun & Johannes Rauschnabel

  3. Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany

    Theresa Anzmann, Peter Gschwind & Reinhard Kohlus

  4. Fraunhofer Institute for Process Engineering and Packaging IVV, Freising, Germany

    Horst-Christian Langowski

Authors
  1. Olga Fysun
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  2. Theresa Anzmann
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  3. Peter Gschwind
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  4. Johannes Rauschnabel
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  5. Reinhard Kohlus
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  6. Horst-Christian Langowski
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Correspondence to Olga Fysun.

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Fysun, O., Anzmann, T., Gschwind, P. et al. Biofilm and dairy fouling detection in flexible tubing using low-field NMR. Eur Food Res Technol 245, 2579–2590 (2019). https://doi.org/10.1007/s00217-019-03371-4

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  • DOI: https://doi.org/10.1007/s00217-019-03371-4

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