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Influence of the use of unripe grapes to reduce ethanol content and pH on the color, polyphenol and polysaccharide composition of conventional and hot macerated Pinot Noir and Tannat wines

Abstract

The aim of this research was to determine the effectiveness of the substitution of grape juice of grapes with different maturation degree and prefermentative hot maceration to obtain Pinot Noir and Tannat red wines with lower alcohol content and pH and higher color and phenolic compound concentrations. Immature grape juice was extracted of grapes harvested in veraison and kept at 4 °C until its use. In technological maturity, the grapes harvested were destemmed, crushed and distributed in 12 containers per cultivar. Six were controls while in the other six 3 L of the original grape juice were substituted by 3 L of unripe grape juice. Next, three containers from each experimental group were traditionally macerated, while the other three were submitted to a prefermentative hot maceration (one hour at 60–70 °C). All treatments performed a fermentative maceration of 7 days. Wines produced from substituted musts had lower alcohol content (14% off) and pH (9% off) and higher titratable acidity (48% more), but no other important changes in wine components were detected. Prefermentative hot maceration increased wine color intensity (50%), total phenolic compounds (66%), total anthocyanin (42%), proanthocyanidin (65%) and polysaccharide (95%) concentrations. The joint consideration of both techniques is an interesting tool to simultaneously mitigate the problems caused by climate change with respect to the maturity of the grape and improve the color of the wine and its concentration in polysaccharides and phenolic compounds.

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References

  1. Boido E, Lloret A, Medina K, Fariña L, Carrau F, Versini G, Dellacassa E (2003) Aroma composition of Vitis vinifera cv. Tannat: the typical red wine from Uruguay. J Agric Food Chem 51:5408–5413

    Article  CAS  PubMed  Google Scholar 

  2. Ó-Marques J, Reguinga R, Laureano O, Ricardo-da-Silva J (2005) Alterações ao longo da maturação nos taninos condensados da grainha, película e polpa: influência damonda de cachos. Ciência Téc Vitiv 20:35–52

    Google Scholar 

  3. Gil M, Kontoudakis N, González E, Esteruelas M, Fort F, Canals JM, Zamora F (2012) Influence of grape maturity and maceration length on color, polyphenolic composition, and polysaccharide content of Cabernet Sauvignon and Tempranillo wines. J Agric Food Chem 60:7988–8001

    Article  CAS  PubMed  Google Scholar 

  4. Fulcrand H, Dueñas M, Salas E, Cheynier V (2006) Phenolic reactions during winemaking and aging. Am J Enol Vitic 57:289–297

    CAS  Google Scholar 

  5. Sacchi K, Bisson L, Adams D (2005) A review of the effect of winemaking techniques on phenolic extraction in red wines. Am J Enol Vitic 56:197–206

    CAS  Google Scholar 

  6. Llaudy MC, Canals R, Canals JM, Zamora F (2008) Influence of ripening stage and maceration length on the contribution of grape skins, seeds and stems to phenolic composition and astringency in wine simulated macerations. Eur Food Res Technol 226:337–344

    Article  CAS  Google Scholar 

  7. Beech FW, Burroughs LF, Timberlake CF, Whiting GC (1979) Progres recents sur l’aspect chimique et antimicrobienne de l’anhidride sulfureux. BullOIV 52:1001–1022

    CAS  Google Scholar 

  8. Jones GV, White MA, Cooper OR (2005) Climate change and global wine quality. Clim Change 73:319–343

    Article  Google Scholar 

  9. Mira de Orduña R (2010) Climate change associated effects on grape and wine quality and production. Food Res Int 43:1844–1855

    Article  CAS  Google Scholar 

  10. Zamora F (2014) Adapting red winemaking to climate change conditions. J Int Sci Vigne Vin Spécial Laccave 71–76

  11. Yu H, Lee O, Lee J, Choi S, Lee J (2005) Quality characteristics and cardiovascular activities of Korean traditional wines and liquors. Food Sci Biotechnol 14:772–777

    CAS  Google Scholar 

  12. Actis-Goretta L, Mackenzie G, Oteiza P, Fraga C (2002) Comparative study on the antioxidant capacity wines and other plant-derived beverages. Ann N Y Acad Sci 957:279–283

    Article  CAS  PubMed  Google Scholar 

  13. González-Neves G, Favre G, Piccardo D (2014) Antocianos y otros compuestos bioactivos característicos de los vinos tintos: incidencia de la variedad de uva y de técnicas de vinificación innovadoras. Revista Facultad Nacional de Agronomía Medellín 67:27–29

    Google Scholar 

  14. Labanda J, Vichi S, Llorens J, Lopez-Tamames E (2009) Membrane separation technology for the reduction of alcoholic degree of white model wine. Food Sci Technol 42:1390–1395

    CAS  Google Scholar 

  15. Bovo B, Nadai C, Vendramini C, Junior WJFL, Carlot M, Skelin A, Giacomina A, Corich V (2016) Aptitude of Saccharomyces yeasts to ferment unripe grapes harvested during cluster thinning for reducing alcohol content of wine. Int J Food Microbiol 236:56–64

    Article  CAS  PubMed  Google Scholar 

  16. Schmidtke LM, Blackman JW, Agboola SO (2012) Production technologies for reduced alcoholic wines. J Food Sci 77:25–41

    Article  CAS  Google Scholar 

  17. Schelezki O, Smith PA, Hranilovica A, Bindon KA, Jeffery DW (2018) Comparison of consecutive harvests versus blending treatments to produce lower alcohol wines from Cabernet Sauvignon grapes: impact on polysaccharide and tannin content and composition. Food Chem 244:50–59

    Article  CAS  PubMed  Google Scholar 

  18. Schelezki OJ, Šukljeb K, Boss PK, Jeffery DW (2018) Comparison of consecutive harvests versus blending treatments to produce lower alcohol wines from Cabernet Sauvignon grapes: impact on wine volatile composition and sensory properties. Food Chem 259:196–206

    Article  CAS  PubMed  Google Scholar 

  19. Sherman E, Greenwood DR, Villas-Boâs SG, Heymann H, Harbertson JF (2017) Impact of grape maturity and ethanol concentration on sensory properties of Washington state Merlot wines. Am J Enol Vitic 68:344–356

    Article  CAS  Google Scholar 

  20. Harbertson JF, Mireles MS, Harwood ED, Weller KM, Ross CF (2009) Chemical and sensory effects of Saignée, water addition, and extended maceration on high brix must. Am J Enol Vitic 60:450–460

    CAS  Google Scholar 

  21. Pickering GJ (2000) Low- and reduced-alcohol wine: a review. J Wine Res 11:129–144

    Article  Google Scholar 

  22. Schultz HR (2000) Climate change and viticulture: a European perspective on climatology, carbon dioxide and UV-B effects. Aust J Grape Wine Res 6:2–12

    Article  CAS  Google Scholar 

  23. Pickering GJ, Heatherbell DA, Barnes MF (1998) Optimising glucose conversion in the production of reduced alcohol wine using glucose oxidase. Food Res Int 31:685–692

    Article  CAS  Google Scholar 

  24. Ciani M, Ferraro L (1996) Enhanced glycerol content in wines made with immobilized Candida stellata cells. Appl Environ Microbiol 62:128–132

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Gómez-Plaza E, López-Nicolás J, López-Roca J, Martinez-Cutillas A (1999) Desalcoholization of wine. Behaviour of the aroma components during the process. Food Sci Technol 32:384–386

    Google Scholar 

  26. Takács L, Vataim G, Korány K (2007) Production of alcohol free wine by pervaporation. J Food Eng 78:118–125

    Article  CAS  Google Scholar 

  27. García-Martín N, Pérez-Magariño S, Ortega-Heras M, González-Huertas C, Mihnea M, González-Sanjosé M, Palacio L, Prádanos P, Hernández A (2010) Sugar reduction in musts with nanofiltration membranes to obtain low alcohol-content wines. Sep Purif Technol 76:158–170

    Article  CAS  Google Scholar 

  28. García-Martín N, Palacio L, Prádanos P, Hernández A, Ortega-Heras M, Perez-Magariño S, González-Huertas C (2009) Evaluation of several ultra- and nanofiltration membranes for sugar control in winemaking. Desalination 245:554–558

    Article  CAS  Google Scholar 

  29. Gil M, Estevez S, Kontoudakis N, Fort F, Canals JM, Zamora F (2013) Influence of partial dealcoholization by reverse osmosis on red wine composition and sensory characteristics. Eur Food Res Technol 237:481–488

    Article  CAS  Google Scholar 

  30. Kontoudakis N, Esteruelas M, Fort F, Canals JM, De Freitas V, Zamora F (2011) Influence of the heterogeneity of grape phenolic maturity on wine composition and quality. Food Chem 124:767–774

    Article  CAS  Google Scholar 

  31. Tinello F, Lante A (2017) Evaluation of anti browning and antioxidant activities in unripe grapes recovered during bunch thinning. Aust J Grape Wine Res 23:33–41

    Article  CAS  Google Scholar 

  32. Geffroy O, Lopez R, Feilhes C, Violleau F, Kleiber D, Favarel JL, Ferreira V (2018) Modulating analytical characteristics of thermovinified Carignan musts and the volatile composition of the resulting wines through the heating temperature. Food Chem 257:7–14

    Article  CAS  PubMed  Google Scholar 

  33. Piccardo D (2015) Evaluación de distintas alternativas en la elaboración de vinos tintos de la variedad Tannat. Tesis de Maestría. Montevideo, Facultad de Agronomía, Uruguay

  34. Piccardo D, González-Neves G (2013) Extracción de polifenoles y composición de vinos tintos Tannat elaborados por técnicas de maceración pre-fermentativas. Agrociencia 17:36–44

    Google Scholar 

  35. El Darra N, Turk F, Ducasse M, Grimi N, Maroun R, Louka N, Vorobiev E (2016) Changes in polyphenol profiles and color composition of freshly fermented model wine due to pulsed electric field, enzymes and thermovinification pretreatments. Food Chem 194:944–950

    Article  CAS  PubMed  Google Scholar 

  36. Andrades-Neves N, Araujo-Panto L, Soares dos Santos A (2014) Thermovinification of grapes from Cabernet sauvignon and Pinot noir varieties using immobilized yeasts. J Food Eng 238:79–84

    Google Scholar 

  37. Baiano A, Terracone G, Gambacorta G, La Notte E (2009) Phenolic content and antioxidant activity of Primitivo wine: comparison among winemaking technologies. J Food Sci 74:258–267

    Article  CAS  Google Scholar 

  38. Atanackovic M, Petrovicm A, Jovic S, Gojkovic-Bukarica L (2012) Influence of winemaking techniques on the resveratrol content, total phenolic content and antioxidant potential of red wines. Food Chem 131:513–518

    Article  CAS  Google Scholar 

  39. Vitis International variety Catalogue. http://www.vivc.de/

  40. OIV (2012) Compendium of international: methods of analysis of wines and musts, vol 1. Organisation Internationale de la Vigne et du Vin, Paris

    Google Scholar 

  41. González-Neves G, Charamelo D, Balado J, Barreiro L, Bochicchio R, Gatto G, Gil G, Tessore A, Carbonneau A, Moutounet M (2004) Phenolic potential of Tannat, Cabernet-Sauvignon and Merlot grapes and their correspondence with wine composition. Anal Chim Acta 513:191–196

    Article  CAS  Google Scholar 

  42. Niketic-Aleksic GK, Hrazdrina G (1972) Quantitative analysis of the anthocyanin content in grape juices and wine. Lebensm Wisss Technol 5:163–165

    CAS  Google Scholar 

  43. Ribéreau-Gayon P, Glories Y, Maujean A, Dubourdieu D (2006) Chap. 6: Phenolic compounds. In: Ribéreau-Gayon P, Glories Y, Maujean A, Dubourdieu D (eds) Handbook of enology. The chemistry of wine stabilisation and treatments, vol 2. Wiley, Chichester pp 141–203

    Chapter  Google Scholar 

  44. Glories Y (1984) La couleur des vins rouges. 2e. Partie: Mesure, origine et interpretation. Connaiss Vigne Vin 18:253–271

    CAS  Google Scholar 

  45. Ayala F, Echávarri JF, Negueruela AI (1997) A new simplified method for measuring the color of wines. I. Red and Rosé Wines. Am J Enol Vitic 48:357–363

    CAS  Google Scholar 

  46. Ayala F, Echávarri JF, Negueruela AI (2001) MSCVes.zip. URL http://www.unizar.es/negueruela/MSCV.es

  47. Valls J (2004) Composició fenòlica en varietats negres de Vitis vinif́era. Influencia de diferents factors, PhD Thesis. Universitat Rovira i Virgili

  48. Kennedy JA, Jones GP (2001) Analysis of proanthocyanidin cleavage products following acid-catalysis in the presence of excess phloroglucinol. J Agric Food Chem 49:1740–1746

    Article  CAS  PubMed  Google Scholar 

  49. Ayestarán B, Guadalupe Z, León D (2004) Quantification of major grape polysaccharides (Tempranillo v.) released by maceration enzymes during the fermentation process. Anal Chim Acta 513:29–39

    Article  CAS  Google Scholar 

  50. González-Neves G, Barreiro L, Gil G, Franco J, Carbonneau A, Moutounet M (2005) Estudio de la composición antociánica de uvas y vinos tintos de los cv. Tannat, Cabernet-Sauvignon y Merlot: utilidad de los perfiles obtenidos para la caracterización varietal. Bull OIV 887–888:30–44

    Google Scholar 

  51. Geffroy O, Lopez R, Serrano E, Dufourcq T, Gracia-Moreno E, Cacho J, Ferreira V (2015) Changes in analytical and volatile compositions of red wines induced by pre-fermentation heat treatment of grapes. Food Chem 187:243–253

    Article  CAS  PubMed  Google Scholar 

  52. Rolle L, Englezos V, Torchio F, Cravero F, Río Sagade S, Ratsiou K, Giacossa S, Gambuti A, Gerbi V, Cocolin L (2017) Alcohol reduction in red wines by technological and microbiological approaches: a comparative study. Aust J Grape Wine Res 24:1–13

    Google Scholar 

  53. El Darra N, Grimi N, Maroun R, Louka N, Vorobiev E (2012) Pulsed electric field, ultrasound, and thermal pretreatments for better phenolic extraction during red fermentation. Eur Food Res Technol 236:47–56

    Article  CAS  Google Scholar 

  54. He F, Liang NN, Mu L, Pan QH, Wang J, Reeves MJ, Duan CQ 2012 Anthocyanins and their variation in red wines I. Monomeric anthocyanins and their color expression. Molecules 17:1571–1601

  55. He F, Liang NN, Mu L, Pan QH, Wang J, Reeves MJ, Duan CQ 2012 Anthocyanins and their variation in red wines II. Anthocyanin derived pigments and their color evolution. Molecules 17:1483–1519

  56. Clarke RJ, Bakker J (2004) Wine flavour chemistry. Blackwell Publishing Oxford

    Book  Google Scholar 

  57. Mazza G, Fukumoto L, Delaquis P, Girard B, Ewert B (1999) Anthocyanins, phenolics and color of Cabernet Franc, Merlot and Pinot Noir wines from British Columbia. J Agric Food Chem 47:4009–4017

    Article  CAS  PubMed  Google Scholar 

  58. Gao L, Girard B, Mazza G, Reynolds AG (1997) Changes in anthocyanins and color characteristics of Pinot Noir wines during different vinification processes. J Agric Food Chem 45:2003–2008

    Article  CAS  Google Scholar 

  59. Cortell JM, Halbleib M, Gallagher AV, Righetti TL, Kennedy JA (2007) Influence of vine vigor on grape (Vitis vinifera L. Cv. Pinot Noir) anthocyanins. Anthocyanins and pigmented polymers in wine. J Agric Food Chem 55:6585–6595

    Article  CAS  PubMed  Google Scholar 

  60. Dimitrovska M, Bocevska M, Dimitrovski D, Murkovic M (2011) Anthocyanin composition of Vranec, Cabernet Sauvignon, Merlot and Pinot Noir grapes as indicator of their varietal differentiation. Eur Food Res Technol 232:591–600

    Article  CAS  Google Scholar 

  61. González-Neves G, Franco J, Barreiro L, Gil G, Moutounet M, Carbonneau A (2007) Varietal differentiation of Tannat, Cabernet-Sauvignon and Merlot grapes and wines according to their anthocyanic composition. Eur Food Res Technol 225:111–117

    Article  CAS  Google Scholar 

  62. Canals R, Llaudy MC, Valls J, Canals JM, Zamora F (2005) Influence of ethanol concentration on the extraction of color and phenolic compounds from the skin and seeds of Tempranillo grapes at different stages of ripening. J Agric Food Chem 53:4019–4025

    Article  CAS  PubMed  Google Scholar 

  63. Souquet JM, Cheynier V, Brossaud F, Moutounet M (1996) Polymeric proanthocyanidins from grape skins. Phytochemistry 43:509–512

    Article  CAS  Google Scholar 

  64. Kennedy JA, Hayasaka Y, Vidal S, Waters EJ, Jones GP (2001) Composition of grape skin proanthocyanidins at different stages of berry development. J Agric Food Chem 49 5348–5355

    Google Scholar 

  65. Souquet JM, Cheynier V, Moutonet M (2000) Les proanthocyanidins du raisin. BullOIV 73:601–609

    CAS  Google Scholar 

  66. Vidal S, Francis L, Guyot S, Marnet N, Kwiatkowski M, Gawel R, Cheynier V, Waters EJ (2003) The mouth-feel properties of grape and apple proanthocyanidins in a wine-like medium. J Sci Food Agric s83:564–573

    Article  CAS  Google Scholar 

  67. Robichaud JL, Noble AC (1990) Astringency and bitterness of selected phenolics in wine. J Sci Food Agric 53:343–353

    Article  CAS  Google Scholar 

  68. Doco T, Williams P, Cheynier V (2007) Effect of flash release and pectinolytic enzyme treatments on wine polysaccharide composition. J Agric Food Chem 55:6643–6649

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The financial support of CAP (Comisión Académica de Posgrado de la Universidad De la República), ANII (Agencia Nacional de Investigación e Innovación, beca MOV_CA_2015_1_107599), CISC (Comisión Sectorial de Investigación Científica, beca de Movilidad, 2017), INAVI (Instituto Nacional de Vitivinicultura), Establecimiento Juanicó y Bodega Olga Silva.

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Correspondence to Fernando Zamora.

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Piccardo, D., Favre, G., Pascual, O. et al. Influence of the use of unripe grapes to reduce ethanol content and pH on the color, polyphenol and polysaccharide composition of conventional and hot macerated Pinot Noir and Tannat wines. Eur Food Res Technol 245, 1321–1335 (2019). https://doi.org/10.1007/s00217-019-03258-4

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Keywords

  • Ethanol reduction
  • pH reduction
  • Unripe grapes
  • Pre-fermentative hot maceration
  • Wine color
  • Wine composition