European Food Research and Technology

, Volume 244, Issue 8, pp 1447–1452 | Cite as

Preparation of diosgenin from Dioscorea zingiberensis C. H. Wright by stepwise biocatalysis-foam separation-preparative high-performance liquid chromatography (P-HPLC)

  • Lei Lei
  • Wei Zhang
  • Zhongchao Gao
  • Long Liu
  • Xiaodong Yu
Original Paper


A novel method of stepwise biocatalysis-foam separation-preparative high-performance liquid chromatography (P-HPLC) to extract dioscin of dioscorea zingiberensis. C. H. Wright (DZW) was presented. Compared with the traditional process of direct hydrolysis of DZW, this novel process reduced the non-saponins material entering the hydrolysis, and, as a result, reduced the consumptions of water, acid, and organic solvent significantly, while simultaneously increasing the saponin yield. In addition, the P-HPLC was more efficient, which can not only get high purity, but also the product yield, high speed, simple operation, and it is convenient for industrialized production preparation.


Diosgenin Stepwise biocatalysis Foam separation Preparative high-performance liquid chromatography 



This research was financially supported by 2017 basic research project Qinghai, China (No. 2017-ZJ-712). The authors thank Prof. Qixiu ZHU of the Key Laboratory of functional organic molecules in Lanzhou University for the assistance in 1HNMR experiments, and Yaling Chi of the Qinghai Normal University for assistance in GC-MS experiments.

Compliance with ethical standards

Conflict of interest

All authors declare that we have no conflict of interest, and the article is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Lei Lei
    • 1
  • Wei Zhang
    • 1
  • Zhongchao Gao
    • 1
  • Long Liu
    • 1
  • Xiaodong Yu
    • 1
  1. 1.School of Chemistry and Chemical EngineeringQinghai Normal UniversityXiningChina

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