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European Food Research and Technology

, Volume 244, Issue 4, pp 577–593 | Cite as

Lysozyme separation from chicken egg white: a review

  • Azin Shahmohammadi
Review Article
  • 398 Downloads

Abstract

Lysozyme (E.C. 3.2.1.17) is a glycoside hydrolase with an important role in antibacterial defense mechanism. Because of its bactericidal and bacteriostatic properties, lysozyme has various applications in the food industry. Therefore, the purification schemes of this enzyme are worth studying. About 3.5% of chicken egg white is lysozyme, so it is one of the best sources of the enzyme. The main purpose of this review is gathering and consolidating scattered information from different studies in the literature and also qualitative and quantitative comparison of the results of different applied methods in the area of lysozyme separation from chicken egg white.

Keywords

Lysozyme Chicken egg white Separation Purification methods 

Abbreviations

AOT

Di-(2-ethylhexyl) sodium sulfosuccinate

ATPS

The aqueous two-phase system

PEG

Polyethylene glycol

EBA

Expanded bed adsorption

EO

Ethylene oxide

PO

Propylene oxide

MPh

Monophenyl trimethoxysilane

ESEW

Ethanol soluble egg whites

EWL

Egg white lysozyme

NFBA

Nd–Fe–B alloy-densified agarose

CB

Cibacron blue 3GA

CB-Streamline

CB-modified Streamline gel

SFB

Stirred fluidized bed

CEW

Chicken egg whites

CEC

Capillary electrochromatography

HSCCC

High-speed counter-current chromatography

RME

Reverse micellar extraction

poly (GMA)

Poly (glycidyl methacrylate)

PEG

Polyethylene glycol

CM-CTS

Carboxymethyl chitosan

PVA-co-PE

Poly(vinyl alcohol-co-ethylene)

IDA

Iminodiacetic acid

CR-PHEMA

Congo red immobilized poly (hydroxyethyl methacrylate)

PHEMA

Poly (hydroxyethyl methacrylate)

UF

Ultrafiltration

TMP

Transmembrane pressure

CPUF

Carrier phase ultrafiltration

PDA

Polydopamine

F-SiO2

Fibrous SiO2

MIP-lysozyme

Magnetic lysozyme-imprinted Fe3O4@F-SiO2@PDA microsphere

HEW

Hen egg white

ATRP

Atom transfer radical polymerization

Notes

Compliance with ethical standards

Conflict of interest

The author declares that he has no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Research Center of oils and fatsFood and Drug Administration, Kermanshah University of Medical sciencesKermanshahIran

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