Effect of allelic variation at glutenin and puroindoline loci on bread-making quality: favorable combinations occur in less toxic varieties of wheat for celiac patients
- 228 Downloads
Genetically diverse wheat samples, twenty-seven Triticum aestivum L. varieties, grown in two environments (Portugal and Spain) were analyzed for their allelic composition in high-molecular-weight glutenin subunits (HMW-GS), low-molecular-weight glutenin subunits (LMW-GS) and puroindolines, as well as their protein content, hardness, sodium dodecyl sulfate-sedimentation (SDS-S), mixograph mixing time and breakdown resistance (MT and BDR, respectively) parameters, and R5 reactivity. The environment showed significant effect on protein content, SDS-S and BDR parameters. In relation to HMW-GS quality effect, the allelic composition Glu-A1d, Glu-B1al, Glu-D1d presented the best results. From the complex Glu-3 loci (LMW-GS), only Glu-B3 locus showed a significant effect on the quality parameters. The Glu-B3ab allele is desirable considering the higher mean values for SDS-S, MT and hardness, and the lower mean values for BDR. Regarding puroindolines, Pina-D1a, Pinb-D1c allelic composition (Leu to Pro at position +60) showed the best quality potential. In addition, we found negative (SDS-S and MT) and positive (BDR) significant correlations between quality parameters and the amount of potential celiac disease toxic epitopes, suggesting that wheat breeding aiming at quality does not have a negative impact on wheat toxicity and on the other hand, emphasizes the need for a more comprehensive wheat breeding programs that encompass celiac disease problematic.
KeywordsWheat quality Glutenins Puroindolines Celiac disease
This work was supported by Grant Acción Integrada Hispano-Portuguesa, HP 2007-0085 and No. AGL 2012-38345 from the Ministerio de Economía y Competitividad in Spain and Acção Luso-Espanhola N.º E-121/08 from Conselho de Reitores das Universidades Portuguesas. Miguel Ribeiro and Luís Pinto have PhD fellowships (SFRH/BD/82334/2011 and SFRH/BD/81307/2011, respectively) granted by the Foundation for Science and Technology (FCT) and European Social Fund (ESF).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
Compliance with ethics requirements
This article does not contain any studies with human or animal subjects.
- 6.Bietz JA, Shepherd KW, Wall JS (1975) Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem 52(4):513–532Google Scholar
- 7.Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35Google Scholar
- 9.Jackson EA, Holt LM, Payne PI (1983) Characterisation of high molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal localisation of their controlling genes. Theor Appl Genet. doi: 10.1007/bf00281844 Google Scholar
- 21.McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Somers DJ, Anderson OA (2008) Catalogue of gene symbols for wheat. http://wheat.pw.usda.gov
- 22.McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat. http://wheat.pw.usda.gov
- 23.Liu L, Ikeda TM, Branlard G, Peña RJ, Rogers WJ, Lerner SE, Kolman MA, Xia X, Wang L, Ma W, Appels R, Yoshida H, Wang A, Yan Y, He Z (2010) Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat. BMC Plant Biol 10(1):1–24. doi: 10.1186/1471-2229-10-124 CrossRefGoogle Scholar
- 25.Ragupathy R, Naeem HA, Reimer E, Lukow OM, Sapirstein HD, Cloutier S (2008) Evolutionary origin of the segmental duplication encompassing the wheat GLU-B1 locus encoding the overexpressed Bx7 (Bx7OE) high molecular weight glutenin subunit. Theor Appl Genet 116(2):283–296. doi: 10.1007/s00122-007-0666-2 CrossRefGoogle Scholar
- 27.AACC (1995) Approved methods of the AACC, 9th edn. Methods 39-11; 39-70A; 76-31; 50-11; 54-30A; 54-40AGoogle Scholar
- 28.Dick JW, Quick JS (1983) A modified screening test for rapid estimation of gluten strength in early generation durum wheat breeding lines. Cereal Chem 60:315–318Google Scholar
- 29.Finney KF, Shogren MD (1972) A ten-gram mixograph for determining and predicting functional properties of wheat flours. Bak Dig 46:32–47Google Scholar
- 36.Surma M, Adamski T, Banaszak Z, Kaczmarek Z, Kuczynska A, Majcher M, Lugowska B, Obuchowski W, Salmanowicz B, Krystkowiak K (2012) Effect of genotype, environment and their interaction on quality parameters of wheat breeding lines of diverse grain hardness. Plant Prod Sci 15(3):192–203. doi: 10.1626/pps.15.192 CrossRefGoogle Scholar
- 37.Dhaliwal AS, Mares DJ, Marshall DR (1987) Effect of 1B/1R chromosome-translocation on milling and quality characteristics of bread wheats. Cereal Chem 64(2):72–76Google Scholar
- 50.Camarca A, Anderson RP, Mamone G, Fierro O, Facchiano A, Costantini S, Zanzi D, Sidney J, Auricchio S, Sette A, Troncone R, Gianfrani C (2009) Intestinal T cell responses to gluten peptides are largely heterogeneous: implications for a peptide-based therapy in celiac disease. J Immunol 182(7):4158–4166. doi: 10.4049/jimmunol.0803181 CrossRefGoogle Scholar
- 51.Ribeiro M, Rodriguez-Quijano M, Nunes FM, Carrillo JM, Branlard G, Igrejas G (2016) New insights into wheat toxicity: breeding did not seem to contribute to a prevalence of potential celiac disease’s immunostimulatory epitopes. Food Chem 213:8–18. doi: 10.1016/j.foodchem.2016.06.043 CrossRefGoogle Scholar