European Food Research and Technology

, Volume 243, Issue 5, pp 743–752 | Cite as

Effect of allelic variation at glutenin and puroindoline loci on bread-making quality: favorable combinations occur in less toxic varieties of wheat for celiac patients

  • Miguel Ribeiro
  • Marta Rodríguez-Quijano
  • Patricia Giraldo
  • Luís Pinto
  • José F. Vázquez
  • Jose Maria Carrillo
  • Gilberto IgrejasEmail author
Original Paper


Genetically diverse wheat samples, twenty-seven Triticum aestivum L. varieties, grown in two environments (Portugal and Spain) were analyzed for their allelic composition in high-molecular-weight glutenin subunits (HMW-GS), low-molecular-weight glutenin subunits (LMW-GS) and puroindolines, as well as their protein content, hardness, sodium dodecyl sulfate-sedimentation (SDS-S), mixograph mixing time and breakdown resistance (MT and BDR, respectively) parameters, and R5 reactivity. The environment showed significant effect on protein content, SDS-S and BDR parameters. In relation to HMW-GS quality effect, the allelic composition Glu-A1d, Glu-B1al, Glu-D1d presented the best results. From the complex Glu-3 loci (LMW-GS), only Glu-B3 locus showed a significant effect on the quality parameters. The Glu-B3ab allele is desirable considering the higher mean values for SDS-S, MT and hardness, and the lower mean values for BDR. Regarding puroindolines, Pina-D1a, Pinb-D1c allelic composition (Leu to Pro at position +60) showed the best quality potential. In addition, we found negative (SDS-S and MT) and positive (BDR) significant correlations between quality parameters and the amount of potential celiac disease toxic epitopes, suggesting that wheat breeding aiming at quality does not have a negative impact on wheat toxicity and on the other hand, emphasizes the need for a more comprehensive wheat breeding programs that encompass celiac disease problematic.


Wheat quality Glutenins Puroindolines Celiac disease 



This work was supported by Grant Acción Integrada Hispano-Portuguesa, HP 2007-0085 and No. AGL 2012-38345 from the Ministerio de Economía y Competitividad in Spain and Acção Luso-Espanhola N.º E-121/08 from Conselho de Reitores das Universidades Portuguesas. Miguel Ribeiro and Luís Pinto have PhD fellowships (SFRH/BD/82334/2011 and SFRH/BD/81307/2011, respectively) granted by the Foundation for Science and Technology (FCT) and European Social Fund (ESF).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Miguel Ribeiro
    • 1
    • 2
  • Marta Rodríguez-Quijano
    • 3
  • Patricia Giraldo
    • 3
  • Luís Pinto
    • 1
    • 2
  • José F. Vázquez
    • 3
  • Jose Maria Carrillo
    • 3
  • Gilberto Igrejas
    • 1
    • 2
    • 4
    Email author
  1. 1.Department of Genetics and BiotechnologyUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  2. 2.Functional Genomics and Proteomics UnitUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  3. 3.UPM Research Group: Plant Breeding, Department of Biotechnology and Plant Biology, School of Agronomic EngineeringPolytechnic University of Madrid (UPM)MadridSpain
  4. 4.UCIBIO-REQUIMTE, Faculty of Science and TechnologyNova University of LisbonLisbonPortugal

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