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Detection of DNA from Escherichia coli, Clostridium perfringens, Staphylococcus aureus and Bacillus cereus after simplified enrichment using a novel multiplex real-time PCR system

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Abstract

A novel multiplex real-time PCR called AllBaktB is presented for the simultaneous determination of DNA from Escherichia coli, Clostridium perfringens, Staphylococcus aureus and Bacillus cereus in food samples. This semi-automated procedure involves a simplified nonselective enrichment and a single isolation step for all bacterial DNA. The results can be gained within 24 h and correspond to the results obtained by the cultivation based on ISO methods. The achieved detection limit was estimated to be at 35 colony forming units per gram food sample for all analytes. A ring trial showed that the method can be transferred to other laboratories showing its robustness.

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Acknowledgments

We thank the cantonal laboratory of Zürich for providing the resources for this work. Special thanks goes to all the laboratories which participated in the Ringtrial like: Swiss Cantonal Laboratories of Aargau (DGSAVS), Zug (AVS), St. Gallen (AVGVS), Thurgau, Zürich and the laboratories in Germany: CVUA Freiburg and LGL Bayern. Furthermore, we would like to thank Gregor McCombie for critically reading the paper.

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  1. Official Food Control Authority of the Canton Zürich, Zürich, Switzerland

    René Köppel, Ingrid Tolido, Gabriela Marti & Martin Peier

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  1. René Köppel
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  2. Ingrid Tolido
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  3. Gabriela Marti
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  4. Martin Peier
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Correspondence to René Köppel.

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Köppel, R., Tolido, I., Marti, G. et al. Detection of DNA from Escherichia coli, Clostridium perfringens, Staphylococcus aureus and Bacillus cereus after simplified enrichment using a novel multiplex real-time PCR system. Eur Food Res Technol 243, 521–530 (2017). https://doi.org/10.1007/s00217-016-2771-4

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  • DOI: https://doi.org/10.1007/s00217-016-2771-4

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