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Simultaneous determination of twenty-five polyphenols in multifloral and cactus honeys using solid-phase extraction and high-performance liquid chromatography with photodiode array detection

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Abstract

Simple and accurate high-performance liquid chromatography–photodiode array detection method has been proposed for simultaneous separation and determination of 25 polyphenol compounds, including 10 phenolic acids, nine flavonoids, and six other phenols in 17 varieties of multifloral and cactus honey of Yemen. Multiwalled carbon nanotubes were used as solid-phase adsorbent for extraction of the polyphenols from honey samples. The total amount of phenolic acids, flavonoids, and other phenols from all 17 analyzed honeys were found in the range of 328–3448, 122–5482, and 2.4–677.6 µg/100 g of honey, respectively. The 4-hydroxybenzoic acid was found in major quantity (10.642 mg/100 g honey) among all analyzed honeys, while chrysin (5.029 mg/100 g honey) was found to be the main detected flavonoid and cinnamic acid the main other phenolic compound (2.330 mg/100 g honey). Among 25 analyzed compounds, 24 were detected (except luteolin) and determined quantitatively in honey samples. The regeneration of solid-phase adsorbent and recovery results showed potential usefulness of the proposed method for the routine analysis of polyphenols in honey extracts.

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References

  1. Kaškonienė V, Maruska A, Kornysova O, Charczun N, Ligor M, Buszewski B (2009) Cheminė Technol 52:74–80

    Google Scholar 

  2. Tofighi Z, Es-haghi A, Asl MM, Tajic AR, Navai MS, Tavakoli S, Hadjiakhoondi A, Yassa N (2014) Eur Food Res Technol 238:665–673

    Article  CAS  Google Scholar 

  3. Tewari JC, Irudayaraj JMK (2005) J Agric Food Chem 53:6955–6966

    Article  CAS  Google Scholar 

  4. Alissandrakis E, Daferera D, Tarantilis PA, Polissiou M, Harizanis PC (2003) Food Chem 82:575–582

    Article  CAS  Google Scholar 

  5. Petrus K, Schwartz H, Sontag G (2011) Anal Bioanal Chem 400:2555–2563

    Article  CAS  Google Scholar 

  6. Martos I, Ferreres F, Yao LD, Arcy B, Caffin N, Tomas-Barberan FA (2000) J Agric Food Chem 48:4744–4748

    Article  CAS  Google Scholar 

  7. Tomas-Barberan FA, Martos I, Ferreres F, Radovic BS, Anklam E (2001) J Sci Food Agric 81:485–496

    Article  CAS  Google Scholar 

  8. Merken HM, Beecher GR (2000) J Agric Food Chem 48:577–599

    Article  CAS  Google Scholar 

  9. Gheldof N, Wang XH, Engeseth NJ (2002) J Agric Food Chem 50:5870–5877

    Article  CAS  Google Scholar 

  10. Wang J, Zhou N, Zhu Z, Huang J, Li G (2007) Anal Bioanal Chem 388:1199–1205

    Article  CAS  Google Scholar 

  11. Khalil MI, Sulaiman SA, Boukraa L (2010) Open Nutraceuticals J 3:6–16

    Article  CAS  Google Scholar 

  12. Lachman J, Hejtmánková A, Sýkora J, Karban J, Orsák M, Rygerová B (2010) Czech J Food Sci 28:412–426

    CAS  Google Scholar 

  13. Lachman J, Orsák M, Hejtmánková A, Kováková E (2010) LWT Food Sci Technol 43:52–58

    Article  CAS  Google Scholar 

  14. Alvarez-Suarez JM, Tulipani S, Romandini S, Bertoli E, Battino M (2010) Mediterr J Nutr Metab 3:15–23

    Article  Google Scholar 

  15. Hadagali MD, Chua LS (2014) Eur Food Res Technol 239:1003–1014

    Article  CAS  Google Scholar 

  16. Phan TT, Wang L, See P, Grayer RJ, Chan SY, Lee ST (2001) Biol Pharm Bull 24:1373–1379

    Article  CAS  Google Scholar 

  17. Cherbuliez T (2001) The medicine from the bees. CD-ROM Apimondia

  18. Cherbuliez T, Domerego R (2003) Lapitherapie, Me´decine des abeilles. Editions Amyris

  19. Amiot MJ, Aubert S, Gonnet M, Tacchini M (1989) Apidologie 20:115–125

    Article  CAS  Google Scholar 

  20. Ferreres F, Tomas-Barberan FA, Gil MI, Tomas-Lorente F (1991) J Sci Food Agric 56:49–56

    Article  CAS  Google Scholar 

  21. Gil MI, Ferreres F, Ortiz A, Subra E, Tomas-Barberan FA (1995) J Agric Food Chem 43:2833–2838

    Article  CAS  Google Scholar 

  22. Martos I, Ferreres F, Tomas-Barberan FA (2000) J Agric Food Chem 48:1498–1502

    Article  CAS  Google Scholar 

  23. Vit P, Soler C, Tomas-Barberan FA (1997) Zeitschrift fur Lebensmittel-Untersuchung und-Forschung A 204:43–47

    Article  CAS  Google Scholar 

  24. Andrade P, Ferreres F, Amaral MT (1997) J Liq Chromatogr Relat Technol 20:2281–2288

    Article  CAS  Google Scholar 

  25. Andrade P, Ferreres F, Gil MI, Tomas-Barberan FA (1997) Determination of phenolic compounds in honeys with different floral origin by capillary zone electrophoresis. Food Chem 60:79–84

    Article  CAS  Google Scholar 

  26. Lu Y, Shen Q, Dai Z, Zhang H (2010) Anal Bioanal Chem 398:1819–1826

    Article  CAS  Google Scholar 

  27. Herrera-Herrera AV, González-Curbelo MA, Hernández-Borges J, Rodríguez-Delgado MA (2012) Anal Chim Acta 734:1–30

    Article  CAS  Google Scholar 

  28. Valcarcel M, Cardenas S, Simonet BM, Moliner-Martinez Y, Lucena R (2008) TrAC 27:34–43

    CAS  Google Scholar 

  29. Yacine BHA, Obeid MS, Wabaidur SM, Alothman ZA (2014) J Agric Food Chem 62:5443–5450

    Article  Google Scholar 

  30. Cai YQ, Jiang GB, Liu JF, Zhou QX (2003) Anal Chim Acta 494:149–156

    Article  CAS  Google Scholar 

  31. Cai Y, Jiang G, Liu J, Zhou Q (2003) Anal Chem 75:2517–2521

    Article  CAS  Google Scholar 

  32. Liang P, Liu Y, Guo L, Zeng J, Lu H (2004) J Anal Atom Spectrom 19:1489–1492

    Article  CAS  Google Scholar 

  33. Yao L, Datta N, Tomas-Barberan FA, Ferreres F, Martos I, Singanusong R (2003) Food Chem 81:159–168

    Article  CAS  Google Scholar 

  34. Martos I, Cossentini M, Ferreres F, Tomas-Barberan FA (1997) J Agric Food Chem 45:2824–2829

    Article  CAS  Google Scholar 

  35. Khan MR, Alothman ZA, Ghafar AA, Wabaidur SM (2013) J Sep Sci 36:572–577

    Article  CAS  Google Scholar 

  36. Wabaidur SM, Alothman ZA, Khan MR (2013) Spectrochim Acta A 108:20–25

    Article  CAS  Google Scholar 

  37. Alothman ZA, Wabaidur SM, Khan MR, Ghafar AA, Habila MA, Yacine BHA (2012) J Sep Sci 35:2892–2896

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project No. RG-1437-004.

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Authors and Affiliations

  1. Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia

    A. Y. Badjah Hadj Ahmed, Saikh M. Wabaidur, Masoom Raza Siddiqui, Zeid A. Alothman, Munir S. Obeid & Mohammad R. Khan

  2. Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia

    Saad A. AL-Tamrah

Authors
  1. A. Y. Badjah Hadj Ahmed
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  2. Saikh M. Wabaidur
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  3. Masoom Raza Siddiqui
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  4. Zeid A. Alothman
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  5. Munir S. Obeid
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  6. Mohammad R. Khan
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  7. Saad A. AL-Tamrah
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Corresponding authors

Correspondence to Saikh M. Wabaidur or Zeid A. Alothman.

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Badjah Hadj Ahmed, A.Y., Wabaidur, S.M., Siddiqui, M.R. et al. Simultaneous determination of twenty-five polyphenols in multifloral and cactus honeys using solid-phase extraction and high-performance liquid chromatography with photodiode array detection. Eur Food Res Technol 242, 943–952 (2016). https://doi.org/10.1007/s00217-015-2601-0

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  • DOI: https://doi.org/10.1007/s00217-015-2601-0

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