European Food Research and Technology

, Volume 242, Issue 4, pp 467–475 | Cite as

Characterisation of selected Croatian honey types based on the combination of antioxidant capacity, quality parameters, and chemometrics

  • Ivana FlanjakEmail author
  • Daniela Kenjerić
  • Dragan Bubalo
  • Ljiljana Primorac
Original Paper


Five honey types produced in Croatia (black locust, lime, sage, chestnut and honeydew) were characterised from the aspect of antioxidant capacity in order to evaluate possible application of antioxidant capacity as a criterion for botanical origin identification. Towards better understanding honey antioxidant capacity, relationship between antioxidant capacity and quality parameters (moisture, electrical conductivity, HMF content, colour, phenolic content) was evaluated using correlation coefficients and principal component analysis (PCA). Antioxidant capacity of analysed honey types varied significantly depending on the botanical origin. Highest antioxidant capacity was determined in dark honeydew and chestnut honey, followed by lime and sage honey, while the lightest black locust honey had the lowest antioxidant capacity. Among evaluated parameters, antioxidant capacity showed highest correlation with phenolic content and colour, with correlation coefficients above 0.89 (p < 0.05). PCA showed that different honey types can be differentiated based on their antioxidant capacity and related parameters.


Unifloral honey Botanical origin Antioxidant capacity Quality parameters Chemometrics 



The authors would like to express their gratitude to Prof Milena Mandić, for scientific advisements and support in all stages of this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human or animal subjects.


  1. 1.
    Frankel S, Robinson GE, Berenbaum MR (1998) Antioxidant capacity and correlated characteristics of 14 unifloral honeys. J Apic Res 37(1):27–31Google Scholar
  2. 2.
    Gheldof N, Wang XH, Engeseth NJ (2002) Identification and quantification of antioxidant components of honey from various floral sources. J Agric Food Chem 50(21):5870–5877CrossRefGoogle Scholar
  3. 3.
    Bertoncelj J, Doberšek U, Jamnik M, Golob T (2007) Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem 105(2):822–828CrossRefGoogle Scholar
  4. 4.
    Pérez RA, Iglesias MT, Pueyo E, González M, de Lorenzo C (2007) Amino acid composition and antioxidant capacity of Spanish honeys. J Agric Food Chem 55(2):360–365CrossRefGoogle Scholar
  5. 5.
    Alvarez-Suarez JM, Tulipani S, Díaz D, Estevez Y, Romandini S, Giampieri F, Damiani E, Astolfi P, Bompadre S, Battino M (2010) Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food Chem Toxicol 48:2490–2499CrossRefGoogle Scholar
  6. 6.
    Brudzynski K, Miotto D (2011) Honey melanoidins: analysis of the compositions of the high molecular weight melanoidins exhibiting radical-scavenging activity. Food Chem 127:1023–1030CrossRefGoogle Scholar
  7. 7.
    Brudzynski K, Miotto D (2011) The relationship between the content of Maillard reaction-like products and bioactivity of Canadian honeys. Food Chem 124:869–874CrossRefGoogle Scholar
  8. 8.
    Brudzynski K, Miotto D (2011) The recognition of high molecular weight melanoidins as the main components responsible for radical-scavenging capacity of unheated and heat-treated Canadian honeys. Food Chem 125:570–575CrossRefGoogle Scholar
  9. 9.
    Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53:1841–1856CrossRefGoogle Scholar
  10. 10.
    Prior RL, Wu X, Schaich K (2005) Standardised methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53(10):4290–4302CrossRefGoogle Scholar
  11. 11.
    Beretta G, Granata P, Ferrero M, Orioli M, Facino RM (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal Chim Acta 533:185–191CrossRefGoogle Scholar
  12. 12.
    Sant’Ana LDO, Sousa JPLM, Salgueiro FB, Lorenzon MCA, Castro RN (2012) Characterisation of monofloral honeys with multivariate analysis of their chemical profile and antioxidant activity. J Food Sci 71(1):C135–C140CrossRefGoogle Scholar
  13. 13.
    Sant’Ana LDO, Ferreira ABB, Lorenzon MCA, Berbara RLL, Castro RN (2014) Correlation of total phenolic and flavonoid contents of Brazilian honeys with colour and antioxidant capacity. Int J Food Prop 17:65–76CrossRefGoogle Scholar
  14. 14.
    Ministry of Agriculture, Fisheries and Rural Development (2009) Regulation on the quality of unifloral honey. Off Gaz 122:15–16Google Scholar
  15. 15.
    Ministry of Agriculture, Fisheries and Rural Development (2009) Regulation on the quality of honey. Off Gaz 93:7–8Google Scholar
  16. 16.
    Codex Alimentarius Commission (2001) Revised codex standard for honey. Alinorm 01(25):19–26Google Scholar
  17. 17.
    Council of the European Union (2002) Council directive 2001/110/EC of Dec 20, 2001, relating to honey. Off J Eur Commun L10:47–52Google Scholar
  18. 18.
    Louveaux J, Maurizio A, Vorwohl G (1978) Methods of melissopalynology. Bee World 59:139–157CrossRefGoogle Scholar
  19. 19.
    Bogdanov S, Martin P, Lüllmann C (1997) Harmonised methods of the European honey commission. Apidologie (extra issue) 28:1–59Google Scholar
  20. 20.
    White JW (2000) In: Graham JM (ed) The hive and the honey bee. Dadant & Sons, Hamilton, pp 869–925Google Scholar
  21. 21.
    Association of Official Analytical Chemists International (AOAC) (2000) Official methods of analysis, 17th edn. Gaithersburg, MD, USA, p 22Google Scholar
  22. 22.
    Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 28:25–30CrossRefGoogle Scholar
  23. 23.
    Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239(1):70–76CrossRefGoogle Scholar
  24. 24.
    Bogdanov S, Rouff K, Persano Oddo L (2004) Physico-chemical methods for the characterisation of unifloral honeys: a review. Apidologie 35:S4–S17CrossRefGoogle Scholar
  25. 25.
    Persano Oddo L, Piro R (2004) Main European unifloral honeys: descriptive sheets. Apidologie 35:S38–S81CrossRefGoogle Scholar
  26. 26.
    Kenjerić D, Primorac LJ, Mandić ML, Bubalo D, Perl Pirički A, Flanjak I (2006) Dalmatian sage (Salvia officinalis L.) honey characterisation. Dtsch Lebensmitt Rundsch 102(10):479–484Google Scholar
  27. 27.
    Kropf U, Jamnik M, Bertoncelj J, Golob T (2008) Linear regression model of the ash mass fraction and electrical conductivity for Slovenian honey. Food Technol Biotech 46(3):335–340Google Scholar
  28. 28.
    Primorac LJ, Flanjak I, Cvijetić M, Đapić Z (2011) Electrical conductivity and ash content of selected honey types. In: Šubarić D (ed) International Scientific and professional conference 13th Ruzicka days “today science-tomorrow industry” proceedings, Osijek, pp 406–411Google Scholar
  29. 29.
    Lazarević KB, Andrić F, Trifković J, Tešić Ž, Milojković-Opsenica D (2012) Characterisation of Serbian unifloral honeys according to their physicochemical parameters. Food Chem 132:2060–2064CrossRefGoogle Scholar
  30. 30.
    United States Department of Agriculture (1985) United States standards for grades of extracted honey. Accessed 24 June 2011
  31. 31.
    Devillers J, Morlot M, Pham-Delégue MH, Doré JC (2004) Classification of monofloral honeys based on their quality control data. Food Chem 86:305–312CrossRefGoogle Scholar
  32. 32.
    Al ML, Daniel D, Moise A, Bobis O, Laslo L, Bogdanov S (2009) Physico-chemical and bioactive properties of different floral origin honeys from Romania. Food Chem 112:863–867CrossRefGoogle Scholar
  33. 33.
    González-Miret ML, Terrab A, Hernanz D, Fernández-Recamales MA, Heredia FJ (2005) Multivariate correlation between color and mineral composition of honeys and by their botanical origin. J Agric Food Chem 53:2574–2580CrossRefGoogle Scholar
  34. 34.
    Mateo Castro R, Jiménez Escamilla M, Bosch Reig M (1992) Evaluation of the color of some Spanish unifloral honey types as a characterization parameter. J AOAC Int 75(3):537–542Google Scholar
  35. 35.
    Terrab A, Gonzalez AG, Diez MJ, Heredia FJ (2003) Characterisation of Moroccan unifloral honeys using multivariate analysis. Eur Food Res Technol 218:88–95CrossRefGoogle Scholar
  36. 36.
    Krpan M, Marković D, Šarić G, Skoko B, Hruškar M, Vahčić N (2009) Antioxidant activities and total phenolics of acacia honey. Czech J Food Sci 27:S245–S247Google Scholar
  37. 37.
    Gheldof N, Engeseth NJ (2002) Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J Agric Food Chem 50(10):3050–3055CrossRefGoogle Scholar
  38. 38.
    Lachman J, Orsák M, Hejtmánková A, Kovářová E (2010) Evaluation of antioxidant activity and total phenolics of selected Czech honeys. LWT Food Sci Technol 43:52–58CrossRefGoogle Scholar
  39. 39.
    Piljac-Žegarac J, Stipčević T, Belščak A (2009) Antioxidant properties and phenolic content of different floral origin honeys. J Apiprod Apimed Sci 1(2):43–50CrossRefGoogle Scholar
  40. 40.
    Jerković I, Marijanović Z (2010) Oak (Quercus frainetto Ten.) honeydew honey-approach to screening of volatile organic composition and antioxidant capacity (DPPH and FRAP assay). Molecules 15:3744–3756CrossRefGoogle Scholar
  41. 41.
    Tuberoso CIG, Jerković I, Bifulco E, Marijanović Z, Congiu F, Bubalo D (2012) Riboflavin and lumichrome in Dalmatian sage honey and other unifloral honeys determined by LC–DAD technique. Food Chem 135:1985–1990CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Ivana Flanjak
    • 1
    Email author
  • Daniela Kenjerić
    • 1
  • Dragan Bubalo
    • 2
  • Ljiljana Primorac
    • 1
  1. 1.Department of Food and Nutrition Research, Faculty of Food Technology OsijekJosip Juraj Strossmayer University of OsijekOsijekCroatia
  2. 2.Faculty of AgricultureUniversity of ZagrebZagrebCroatia

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