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Synergistic and antagonistic effects between alpha-tocopherol and phenolic acids in liposome system: spectroscopic study

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Abstract

The physicochemical interactions between alpha-tocopherol and three phenolic acids, which constitute main phenolics in coffee brew: caffeic, chlorogenic and ferulic acid, in l-α-phosphatidylcholine liposome system were studied. Steady-state and fluorescence lifetime measurements were applied to elucidate location of investigated phenolic acids in liposomes, and the results have shown that ferulic acid is most embedded into membrane structure. Lipophilic studies have shown that at pH 7.4 the partition coefficients for all phenolic acids are similar. Antioxidant capacity measurements of studied antioxidants were taken using fluorescent probe BODIPY. The synergistic effect was observed in all tested antioxidant systems with the exception of sample consisting of chlorogenic acid (2.5 μM) and alpha-tocopherol (2.5 μM), where antagonistic effect was noted. Concentration of antioxidants was a significant factor in the observed phenomenon. The most effective antioxidant system against oxidation in liposomes was combination of alpha-tocopherol and ferulic acid. This phenomenon could be explained by interaction of ferulic acid with the interior of the phospholipids membrane.

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Abbreviations

AAPH:

2,2′-Azobis(2-amidinopropane) dihydrochloride

α-T:

Alpha-tocopherol

C11-BODIPY581/591:

4,4-Difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid

CA:

Caffeic acid

CGA:

Chlorogenic acid

C OB :

Partition coefficient

DPH:

1,6-Diphenyl-1,3,5-hexatriene

FA:

Ferulic acid

PCF:

Protection coefficient

PC:

l-α-Phosphatidylcholine

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Acknowledgments

This study was partially supported by a Grant 508/82-4 from Poznan University of Life Sciences, Poznan, Poland, and by a Grant N312 1410 33 from the Polish Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Department of Physics, Poznan University of Life Sciences, Wojska Polskiego 38/42, 60-637, Poznan, Poland

    Grażyna Neunert & Krzysztof Polewski

  2. Latvia State Institute of Fruit-Growing, Graudu 1, Dobele, 3701, Latvia

    Paweł Górnaś

  3. Department of Food Biochemistry and Analysis, Poznan University of Life Sciences, Wojska Polskiego 48, 60-637, Poznan, Poland

    Krzysztof Dwiecki & Aleksander Siger

Authors
  1. Grażyna Neunert
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  2. Paweł Górnaś
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  3. Krzysztof Dwiecki
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  4. Aleksander Siger
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  5. Krzysztof Polewski
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Correspondence to Grażyna Neunert.

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Neunert, G., Górnaś, P., Dwiecki, K. et al. Synergistic and antagonistic effects between alpha-tocopherol and phenolic acids in liposome system: spectroscopic study. Eur Food Res Technol 241, 749–757 (2015). https://doi.org/10.1007/s00217-015-2500-4

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  • DOI: https://doi.org/10.1007/s00217-015-2500-4

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