Direct DNA amplification from virgin olive oil for traceability and authenticity


The olive tree is one of the most common fruit species cultivated for oil and table olives in Italy and, particularly, in the Mediterranean area. DNA fingerprinting methods include different markers; however, our work is based on the identification of olive oil cultivars by using simple sequence repeats analysis. As previously reported in the literature, this proposed method shows good capability to amplify, for example, DNA from wine and table grape varieties. In our paper, we suggest an easy methodology, which allows direct amplification of DNA and bypassing of the extraction of DNA using an engineered DNA polymerase, KAPA3G Plant DNA polymerase, improved to tolerate plant PCR inhibitors. This new procedure is more efficient, faster and cheaper than traditional methods of DNA extraction and amplification and leads to more accurate results. This innovative protocol, without the addition of chemical solutions, has provided good results and has permitted traceability of virgin olive oils.

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Fig. 1



Certification of the varietal composition, geographic origin and the absence of synthetic products in the extra virgin olive oils


Deoxyribonucleic acid


Polymerase chain reaction


Protected designation of origin


Protected geographical indication


Registered designation of origin


Simple sequence repeat


Virgin olive oil


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This research was supported by the ‘Certification of the varietal composition, geographic origin and the absence of synthetic products in the extra virgin olive oilsCERTOLIO 2012–2015’ and by the ‘Advanced technological systems and processes embedded in the olive oil sector for the development of products and by-products, the development of new areas and the creation of environmentally friendly production systems’ OLIOPIU’ 2011–2015 projects.

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All the authors declare that there is no conflict of interests regarding the publication of this article.

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This article does not contain any studies with human or animal subjects.

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Correspondence to Innocenzo Muzzalupo.

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Muzzalupo, I., Pisani, F., Greco, F. et al. Direct DNA amplification from virgin olive oil for traceability and authenticity. Eur Food Res Technol 241, 151–155 (2015).

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  • SSR loci
  • Extra virgin olive oil
  • Direct DNA amplification
  • Engineered DNA polymerase