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Direct DNA amplification from virgin olive oil for traceability and authenticity

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Abstract

The olive tree is one of the most common fruit species cultivated for oil and table olives in Italy and, particularly, in the Mediterranean area. DNA fingerprinting methods include different markers; however, our work is based on the identification of olive oil cultivars by using simple sequence repeats analysis. As previously reported in the literature, this proposed method shows good capability to amplify, for example, DNA from wine and table grape varieties. In our paper, we suggest an easy methodology, which allows direct amplification of DNA and bypassing of the extraction of DNA using an engineered DNA polymerase, KAPA3G Plant DNA polymerase, improved to tolerate plant PCR inhibitors. This new procedure is more efficient, faster and cheaper than traditional methods of DNA extraction and amplification and leads to more accurate results. This innovative protocol, without the addition of chemical solutions, has provided good results and has permitted traceability of virgin olive oils.

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Fig. 1

Abbreviations

CERTOLIO:

Certification of the varietal composition, geographic origin and the absence of synthetic products in the extra virgin olive oils

DNA:

Deoxyribonucleic acid

PCR:

Polymerase chain reaction

PDO:

Protected designation of origin

PGI:

Protected geographical indication

RDO:

Registered designation of origin

SSR:

Simple sequence repeat

VOO:

Virgin olive oil

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Acknowledgments

This research was supported by the ‘Certification of the varietal composition, geographic origin and the absence of synthetic products in the extra virgin olive oils—CERTOLIO 2012–2015’ and by the ‘Advanced technological systems and processes embedded in the olive oil sector for the development of products and by-products, the development of new areas and the creation of environmentally friendly production systems’ OLIOPIU’ 2011–2015 projects.

Conflict of interest

All the authors declare that there is no conflict of interests regarding the publication of this article.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Consiglio per la Ricerca e sperimentazione per l’Agricoltura, centro di ricerca per l’olivicoltura e l’industria olearia (CRA-OLI), C/da Li Rocchi-Vermicelli, 87036, Rende, CS, Italy

    Innocenzo Muzzalupo, Francesca Pisani & Federica Greco

  2. ISOLAB srl - Centro Servizi l’Acquario, Via Ciccarello 77, 89132, Reggio Calabria, Italy

    Federica Greco

  3. Dipartimento di Biologia, Ecologia e Scienza della Terra (DiBEST), Università della Calabria, Ponte P. Bucci, 87036, Arcavacata di Rende, CS, Italy

    Adriana Chiappetta

Authors
  1. Innocenzo Muzzalupo
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  2. Francesca Pisani
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  3. Federica Greco
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  4. Adriana Chiappetta
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Corresponding author

Correspondence to Innocenzo Muzzalupo.

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Muzzalupo, I., Pisani, F., Greco, F. et al. Direct DNA amplification from virgin olive oil for traceability and authenticity. Eur Food Res Technol 241, 151–155 (2015). https://doi.org/10.1007/s00217-015-2455-5

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  • DOI: https://doi.org/10.1007/s00217-015-2455-5

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