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Composition of tocochromanols in kernels recovered from different sweet cherry (Prunus avium L.) cultivars: RP-HPLC/FLD and RP-UPLC-ESI/MSn study

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Abstract

Tocochromanols profile in kernels recovered from fruit pits of seven sweet cherry (Prunus avium L., family: Rosaceae) cultivars was studied. Four tocopherol homologues (α-T, β-T, γ-T, and δ-T) were quantified in all tested samples by RP-HPLC/FLD method. The RP-UPLC-ESI/MSn analyses allowed to detect trace amounts of two tocotrienol homologues (α and γ). The concentration of individual tocopherol homologues in kernels of different sweet cherry cultivars, expressed in mg/100 g dwb, was in the follow range: 3.03–4.96 (α-T), 0.01–0.11 (β-T), 32.26–37.51 (γ-T), and 0.95–1.69 (δ-T). The γ-T was the main tocopherol homologue identified in sweet cherry kernels and constituted approximately 88 % of total detected tocochromanols. The percentage composition of the individual tocopherol homologues in kernels of different sweet cherry cultivars was comparable.

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Abbreviations

RP-HPLC/FLD:

Reverse-phase high-performance liquid chromatograph/fluorescence detector

RP-UPLC-ESI/MSn :

Reverse-phase ultra-performance liquid chromatography–electrospray ionization/mass spectrometry

T:

Tocopherol

T3:

Tocotrienol

SD:

Standard deviation

dwb:

Dry-weight basis

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Acknowledgments

This research was supported by the National Research Programme of Latvia “AgroBioRes.” I would like to kindly acknowledge Dr. Arianne Soliven for her assistance.

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Latvia State Institute of Fruit-Growing, Graudu 1, Dobele, 3701, Latvia

    Paweł Górnaś, Inga Mišina, Silvija Ruisa, Edgars Rubauskis, Gunārs Lācis & Dalija Segliņa

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  1. Paweł Górnaś
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  2. Inga Mišina
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  3. Silvija Ruisa
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  4. Edgars Rubauskis
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Correspondence to Paweł Górnaś.

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Górnaś, P., Mišina, I., Ruisa, S. et al. Composition of tocochromanols in kernels recovered from different sweet cherry (Prunus avium L.) cultivars: RP-HPLC/FLD and RP-UPLC-ESI/MSn study. Eur Food Res Technol 240, 663–667 (2015). https://doi.org/10.1007/s00217-014-2382-x

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  • DOI: https://doi.org/10.1007/s00217-014-2382-x

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