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A new sensitive method for the detection of chloramphenicol in food using time-resolved fluoroimmunoassay

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Abstract

A time-resolved fluoroimmunoassay (TRFIA) technique was developed to detect chloramphenicol (CAP) contamination in food. By using CAP-ovalbumin, anti-CAP antibodies, and europium-labelled goat anti-rabbit antibodies, an indirect, competitive method for CAP-TRFIA was established. The sensitivity was high, with a detection limit of 0.008 μg/L (8 ppt) for indirect competitive TRFIA formats. Testing showed that the sensitivity of the technique was 2.67 μg/kg in honey, prawn, and chicken muscle tissues, and 0.32 μg/L in milk. The detection range was between 0.008 and 100 μg/L: within this, the intra- and inter-batch coefficients of variation of the CAP-TRFIA method were 6.8 and 13.5 %, respectively. The study suggested that CAP-TRFIA was a simple, sensitive, and cost-effective method of screening large quantities of samples and had good prospects for further application.

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Compliance with Ethics Requirements

This article does not contain any studies with human subjects. All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Ministry of Health, Wuxi, 214063, Jiangsu, People’s Republic of China

    Bin Zhou, Jue Zhang, Jun Fan, Lan Zhu, Yi Zhang & Biao Huang

  2. School of Medicine and Pharmaceutics, Southern Yangtze University, Wuxi, 214063, Jiangsu, People’s Republic of China

    Jian Jin

Authors
  1. Bin Zhou
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  2. Jue Zhang
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  3. Jun Fan
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  4. Lan Zhu
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  5. Yi Zhang
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  6. Jian Jin
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  7. Biao Huang
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Correspondence to Biao Huang.

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Zhou, B., Zhang, J., Fan, J. et al. A new sensitive method for the detection of chloramphenicol in food using time-resolved fluoroimmunoassay. Eur Food Res Technol 240, 619–625 (2015). https://doi.org/10.1007/s00217-014-2363-0

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  • DOI: https://doi.org/10.1007/s00217-014-2363-0

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