Impact of a 6-week very low-calorie diet and weight reduction on the serum and fecal metabolome of overweight subjects
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The aim of this study was to elucidate the effects of a very low-calorie diet and weight loss on the serum and fecal metabolome and the potential of the metabolome to predict inter-individual differences in body weight loss. NMR-based metabolomics was carried out on serum and fecal samples obtained from healthy female subjects (n = 56; Age: 46.33 ± 10.39 years; BMI 28.32 ± 1.55 kg/m2) pre- and post-weight reduction. An elevated level of 3-hydroxybutyric acid (3-HBA) and acetoacetate and decreased levels of lipoproteins, cholines and glucose were identified in serum after the weight reduction. In the fecal metabolome, a decreased level of short-chain fatty acids was observed after the weight reduction. The body weight for each individual at pre- and post-intervention was linked to the level of lipoproteins in serum (VLDL, p = 0.039; LDL, p = 0.023) and serum 3-HBA (p < 0.001), and a tendency for a similar relation was found for the fecal concentration of acetate (p = 0.06) and propionate (p = 0.075). The study demonstrates that the serum and fecal metabolome is affected by weight loss and that it includes information about inter-individual differences at the post-genomic level that may be of importance for the ability to undergo a weight loss.
KeywordsCalorie restriction Metabolic phenotype Nuclear magnetic resonance Very low-calorie diet Weight loss
Arla Foods Nordic Innovation is thanked for the collaborative work behind this study. Also, Good Food Practice® is thanked for handling sample collection and clinical measurements of subjects. The Danish Research Council is thanked for financial support through the project ‘Advances in food quality and nutrition research through implementation of metabolomics technologies’ (#274-09-107).
Conflict of interest
The authors declare that they have no conflict of interest.
Compliance with Ethics Requirements
All procedures followed were in accordance with the EU Clinical Directive 2001/20/EC and ICH Guideline for Good Clinical Practice. Informed consent was obtained from the subjects included in the study.
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