Impact of a 6-week very low-calorie diet and weight reduction on the serum and fecal metabolome of overweight subjects
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The aim of this study was to elucidate the effects of a very low-calorie diet and weight loss on the serum and fecal metabolome and the potential of the metabolome to predict inter-individual differences in body weight loss. NMR-based metabolomics was carried out on serum and fecal samples obtained from healthy female subjects (n = 56; Age: 46.33 ± 10.39 years; BMI 28.32 ± 1.55 kg/m2) pre- and post-weight reduction. An elevated level of 3-hydroxybutyric acid (3-HBA) and acetoacetate and decreased levels of lipoproteins, cholines and glucose were identified in serum after the weight reduction. In the fecal metabolome, a decreased level of short-chain fatty acids was observed after the weight reduction. The body weight for each individual at pre- and post-intervention was linked to the level of lipoproteins in serum (VLDL, p = 0.039; LDL, p = 0.023) and serum 3-HBA (p < 0.001), and a tendency for a similar relation was found for the fecal concentration of acetate (p = 0.06) and propionate (p = 0.075). The study demonstrates that the serum and fecal metabolome is affected by weight loss and that it includes information about inter-individual differences at the post-genomic level that may be of importance for the ability to undergo a weight loss.
KeywordsCalorie restriction Metabolic phenotype Nuclear magnetic resonance Very low-calorie diet Weight loss
Arla Foods Nordic Innovation is thanked for the collaborative work behind this study. Also, Good Food Practice® is thanked for handling sample collection and clinical measurements of subjects. The Danish Research Council is thanked for financial support through the project ‘Advances in food quality and nutrition research through implementation of metabolomics technologies’ (#274-09-107).
Conflict of interest
The authors declare that they have no conflict of interest.
Compliance with Ethics Requirements
All procedures followed were in accordance with the EU Clinical Directive 2001/20/EC and ICH Guideline for Good Clinical Practice. Informed consent was obtained from the subjects included in the study.
- 1.WHO (2013) http://www.who.int/mediacentre/factsheets/fs311/en/. Obesity and overweight
- 7.Dumas M-E, Maibaum EC, Teague C, Ueshima H, Zhou B, Lindon JC, Nicholson JK, Stamler J, Elliott P, Chan Q, Holmes E (2006) Assessment of analytical reproducibility of 1H NMR spectroscopy based metabonomics for large-scale epidemiological research: the INTERMAP study. Anal Chem 78:2199–2208CrossRefGoogle Scholar
- 11.Moazzami AA, Zhang J-X, Kamal-Eldin A, Åman P, Hallmans G, Johansson J-E, Andersson S-O (2011) Nuclear magnetic resonance-based metabolomics enable detection of the effects of a whole grain rye and rye bran diet on the metabolic profile of plasma in prostate cancer patients. J Nutr 141:2126–2136CrossRefGoogle Scholar
- 12.Maccaferri S, Klinder A, Cacciatore S, Chitarrari R, Honda H, Luchinat C, Bertini I, Carnevali P, Gibson GR, Brigidi P, Costabile A (2012) In vitro fermentation of potential prebiotic flours from natural sources: impact on the human colonic microbiota and metabolome. Mol Nutr Food Res 56:1342–1352CrossRefGoogle Scholar
- 14.Claus SP, Ellero SL, Berger B, Krause L, Bruttin A, Molina J, Paris A, Want EJ, de Waziers I, Cloarec O, Richards SE, Wang Y, Dumas ME, Ross A, Rezzi S, Kochhar S, Van Bladeren P, Lindon JC, Holmes E, Nicholson JK (2011) Colonization-induced host-gut microbial metabolic interaction. mBio 2(2):e00271–00210. doi: 10.1128/mBio.00271-10
- 17.German JB, Bauman DE, Burrin DG, Failla M, Failla ML, Freake HC, King JC, Klein S, Milner JA, Pelto GH, Rasmussen KM, Zeisel SH (2004) Metabolomics in the opening decade of the 21st century: building the roads to individualized health. J Nutr 134:2729–2732Google Scholar
- 22.Dattilo AM, Kris-Etherton PM (1992) Effects of weight reduction on blood lipids and lipoproteins: a metaanalysis. Am J Clin Nutr 56:320–328Google Scholar
- 23.Malandrucco I, Pasqualetti P, Giordani I, Manfellotto D, De Marco F, De Alegiani F, Sidoti AM, Picconi F, Di Flaviani A, Frajese G, Bonadonna RC, Frontoni S (2012) Very-low-calorie diet: a quick therapeutic tool to improve b cell function in morbidly obese patients with type 2 diabetes. Am J Clin Nutr 95:609–613CrossRefGoogle Scholar
- 24.Svendsen PF, Jensen FK, Holst JJ, Haugaard SB, Nilas L, Madsbad S (2012) The effect of a very low calorie diet on insulin sensitivity, beta cell function, insulin clearance, incretin hormone secretion, androgen levels and body composition in obese young women. Scand J Clin Lab Invest 72:410–419CrossRefGoogle Scholar
- 35.Ala-Korpela M, Korhonen A, Keisala J, Hörkkö S, Korpi P, Ingman LP, Jokisaari J, Savolainen MJ, Kesäniemi YA (1994) 1H NMR-based absolute quantification of humans lipoproteins and their lipid contents directly from plasma. J Lipid Res 35(12):2292–2304Google Scholar
- 36.Otvos JD, Jeyarajah EJ, Bennett DW (1991) Quantification of plasma lipoproteins by proton nuclear magnetic resonance spectroscopy. Clin Chem 37(3):377–386Google Scholar
- 37.Chearskul S, Delbridge E, Shulkes A, Proietto J, Kriketos A (2008) Effect of weight loss and ketosis on postprandial cholecystokinin and free fatty acid concentrations. Am J Clin Nutr 87:1238–1246Google Scholar
- 40.Volek JSS, Matthew J, Forsythe CE (2005) Modification of lipoproteins by very low-carbohydrate diets 1. J Nutr 135:1339–1342Google Scholar
- 42.Gibney MJ, Walsh M, Brennan L, Roche HM, German B, van Ommen B (2005) Metabolomics in human nutrition: opportunities and challenges. Am J Clin Nutr 82:497–503Google Scholar
- 44.Astrup A, Buemann B, Gluud C, Bennett P, Tjur T, Christensen N (1995) Prognostic markers for diet-induced weight-loss in obese women. Int J Obes 19(4):275–278Google Scholar
- 48.Macfarlane G, Gibson G (1997) Carbohydrate fermentation, energy transduction and gas metabolism in the human large intestine. In: Mackie R, White B (eds) Gastrointestinal microbiology. Chapman & Hall Microbiology Series. Springer, USA, pp 269–318. doi: 10.1007/978-1-4615-4111-0_9
- 52.Al-Lahham SH, Roelofsen H, Priebe M, Weening D, Dijkstra M, Hoek A, Rezaee F, Venema K, Vonk RJ (2010) Regulation of adipokine production in human adipose tissue by propionic acid. Eur J Clin Invest 40(5):401–407Google Scholar