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Punicalagin exhibits negative regulatory effects on LPS-induced acute lung injury

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Abstract

Punicalagin, mainly isolated from the fruit of Pomegranate (Punica granatum L.), is a natural polyphenolic compound. In the present study, we investigated the negative regulatory effect of punicalagin on acute lung injury (ALI) induced by Lipopolysaccharide (LPS). In the murine model of ALI, the data showed that punicalagin inhibited the production of TNF-α, IL-1β, and IL-6 and decreased protein concentration and myeloperoxidase activity with a single 4 mg/kg dose of punicalagin prior to the administration of intratracheal LPS in the bronchoalveolar lavage fluid. Furthermore, we investigated the effects of punicalagin how to modulate signal transduction. MAPK and NF-κB activation were measured by Western blot and immunocytochemical analysis. The data showed that punicalagin significantly inhibited phosphorylated p38 MAPK protein expression and shocked p65-NF-κB translocation into the nucleus. These results indicated punicalagin may exert negative regulatory effects on ALI partly through suppressing p38 MAPKs or/and NF-κB pathways. This study offered a novel therapeutic strategy for improving clinical effects of acute lung injury (ALI)/acute respiratory distress syndrome and provided more evidence for the health benefits of pomegranate fruits.

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Acknowledgments

This study was supported by the Post-doctor Science Fund of China. Number: 801120106201.

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None.

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This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Laboratory of Nutrition and Function Food, College of Light Industry Economics and Management, Jilin University, Changchun, 130062, Jilin, People’s Republic of China

    Shuang Guan, Zhenning Wang, Yixuan Huang, Guoren Huang, Wei Jiang & Jing Lu

  2. Key Laboratory of Zoonosis, Ministry of Education College of Veterinary Medicine, Jilin University, Changchun, 130062, Jilin, People’s Republic of China

    Shuang Guan & Jing Lu

  3. Department of Chemical Engineering and Technology, College of Science and Technology, Yanbian University, Yanji, 133002, Jilin, People’s Republic of China

    Yuekai Guan

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  1. Shuang Guan
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  2. Zhenning Wang
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  3. Yixuan Huang
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  4. Guoren Huang
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  5. Yuekai Guan
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  6. Wei Jiang
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  7. Jing Lu
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Correspondence to Jing Lu.

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Guan, S., Wang, Z., Huang, Y. et al. Punicalagin exhibits negative regulatory effects on LPS-induced acute lung injury. Eur Food Res Technol 239, 837–845 (2014). https://doi.org/10.1007/s00217-014-2280-2

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  • DOI: https://doi.org/10.1007/s00217-014-2280-2

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