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Analysis of ERIC-PCR genomic polymorphism of Salmonella isolates from chicken slaughter line

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Abstract

The chicken slaughter line is a source of cross-contamination of Salmonella. In this study, ERIC-PCR was applied to analyse the ERIC-PCR genomic polymorphism of Salmonella isolates from a commercial chicken slaughter line and to trace the route of contamination. Samples were collected from carcasses and contact surfaces at the points of post-evisceration, post-chilling and post-grading. The prevalence of Salmonella at the evisceration point was high but significantly decreased along the slaughter line. The ERIC-PCR fingerprints indicated that a total of seven groups were clustered, and the genotypic diversity of isolates progressively decreased along the slaughter line. By tracing the genotypic diversity, contact surfaces at post-evisceration were found to be the major contamination sources of Salmonella during chicken processing, since the genotype diversity of isolates from the post-evisceration point could be exactly matched to that from the post-chilling and post-grading points. Interestingly, three Salmonella strains were still detected after decontamination and washing; these three isolates having a strong capacity for attachment were able to produce biofilm on polystyrene surfaces. This study suggests that the evisceration point is the source of cross-contamination, with Salmonella isolates still present after washing procedure. Therefore, more effective measures must be undertaken to control the spread of Salmonella in such processing lines.

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Acknowledgments

This study was supported by China Agriculture Research System (CARS-42) funded by the China Ministry of Agriculture. We are very grateful to Prof. Ron Tume from CSIRO, Animal, Food and Health Sciences, Australia, for his valuable advice and for assistance with language.

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Huhu Wang, Ying Zhao, Qiuqin Zhang, Xinglian Xu & Guanghong Zhou

  2. Xi’an Institute for Food and Drug Control, Xi’an, 710054, People’s Republic of China

    Ruihua Shu

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  1. Huhu Wang
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  2. Ruihua Shu
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  3. Ying Zhao
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  4. Qiuqin Zhang
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  5. Xinglian Xu
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  6. Guanghong Zhou
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Correspondence to Huhu Wang.

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Wang, H., Shu, R., Zhao, Y. et al. Analysis of ERIC-PCR genomic polymorphism of Salmonella isolates from chicken slaughter line. Eur Food Res Technol 239, 543–548 (2014). https://doi.org/10.1007/s00217-014-2277-x

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  • DOI: https://doi.org/10.1007/s00217-014-2277-x

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