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High-resolution melting analysis: a promising molecular method for meat traceability

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Abstract

To find a promising molecular method for meat traceability, three methods of single nucleotide polymorphism (SNP) detection: RFLP-PCR analysis, high-resolution melting (HRM) analysis, and TaqMan probe analysis, have been compared in terms of accuracy, ease of use, throughput capability, and cost. We genotyped ten pork DNA samples across three SNPs. The results showed that the HRM genotyping method was the most accurate and easiest to use with the lowest cost, while TaqMan probe analysis provided similar results, but its cost was much higher.

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Acknowledgments

This work was supported by Grants 2012BAD28B02-03 (MOST), 2012BAK17B07 (MOST), NCET-11-0668 (MOE), and CARS-36-11 (MOA).

Conflict of interest

None.

Compliance with Ethics Requirements

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2008 (5). Informed consent was obtained from all patients for being included in the study.

All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Key Laboratory of Animal Products Processing, MOA, Key Laboratory of Meat Processing and Quality Control, MOE, Synergetic Innovation Center of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agricultural University, Weigang NO. 1, Nanjing, 210095, Jiangsu, China

    Sasa Yang, Chunbao Li, Qiayu Wu, Changqing Zhu, Xinglian Xu & Guanghong Zhou

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  1. Sasa Yang
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  2. Chunbao Li
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  3. Qiayu Wu
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  4. Changqing Zhu
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  5. Xinglian Xu
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  6. Guanghong Zhou
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Corresponding author

Correspondence to Guanghong Zhou.

Additional information

Sasa Yang and Chunbao Li have contributed equally to this work.

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Yang, S., Li, C., Wu, Q. et al. High-resolution melting analysis: a promising molecular method for meat traceability. Eur Food Res Technol 239, 473–480 (2014). https://doi.org/10.1007/s00217-014-2241-9

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  • DOI: https://doi.org/10.1007/s00217-014-2241-9

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