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Phytoestrogenic activity of Aceriphyllum rossii and rapid identification of phytoestrogens by LC–NMR/MS and bioassay-guided isolation

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Abstract

Aceriphyllum rossii Engler (Saxifragaceae) has been used as a nutritious food in Korea, but only minimal studies on this plant have been undertaken. We have examined the estrogenic effect of A. rossii and have discovered its potent constituents. To identify the estrogenic activity of A. rossii, we measured the transactivation of the estrogen-responsive element (ERE) in MCF-7 cells transfected with an ERE-containing construct after treatment of the extract and fractions of A. rossii. A. rossii showed a potent estrogenic activity and gallic acid ethyl ester; quercetin and kaempferol were identified as major constituents of the active fractions by liquid chromatography–nuclear magnetic resonance spectroscopy/mass spectrometry. We confirmed quercetin and kaempferol are the major active constituents after activity-guided isolation of seven compounds: gallic acid ethyl ester (1), quercetin (2), kaempferol (3), quercetin-3-O-(6″-galloyl)-β-d-glucopyranoside (4), quercetin-3-O-β-d-glucopyranoside (5), kaempferol-3-O-(6″-galloyl)-β-d-glucopyranoside (6), and kaempferol-3-O-β-d-glucopyranoside (7). These results show A. rossii could be a possible candidate for the treatment of postmenopausal symptoms.

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Acknowledgments

This work was supported by Korea Institute of Science and Technology intramural grant (2Z03850).

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Functional Food Center, KIST Gangneung Institute, Gangwon, 210–340, Republic of Korea

    Suk Woo Kang, Kyungsu Kang, Mi Ae Kim, Na Ra Jeon, Sang Min Kim, Chu Won Nho & Byung-Hun Um

  2. College of Pharmacy, Hanyang University, Ansan, Korea

    Je-Seung Jeon

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  1. Suk Woo Kang
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  2. Kyungsu Kang
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  3. Mi Ae Kim
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  4. Na Ra Jeon
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Correspondence to Suk Woo Kang.

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Kang, S.W., Kang, K., Kim, M.A. et al. Phytoestrogenic activity of Aceriphyllum rossii and rapid identification of phytoestrogens by LC–NMR/MS and bioassay-guided isolation. Eur Food Res Technol 239, 237–246 (2014). https://doi.org/10.1007/s00217-014-2212-1

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  • DOI: https://doi.org/10.1007/s00217-014-2212-1

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