European Food Research and Technology

, Volume 238, Issue 1, pp 157–162 | Cite as

Extremely low-frequency magnetic fields affect pigment production of Monascus purpureus in liquid-state fermentation

  • J. Zhang
  • K. Zhou
  • L. Wang
  • M. Gao
Original Paper


Red pigment is one of natural coloring agents produced from the secondary metabolites of Monascus purpureus. Measures are taken to increase the pigment production. Effect of the extremely low-frequency magnetic field on the red and yellow pigment production of M. purpureus in liquid-state fermentation was estimated by exposing fermentation broth, medium and sterile distilled water used to prepare medium. The maximum yield of red and yellow pigment production had about 20 and 36 % increase for 8 days at 0.4 mT when treating fermentation broth compared to the control value. For sterile distilled water, a significant increase occurred at 0.4 mT compared to 0.1 mT for 5 h. However, the red and yellow pigment production decreased significantly at magnetic fields of 0.9 and 1.2 mT when treating fermentation broth and sterile distilled water. In contrast, the yield of both red and yellow pigment production decreased significantly compared with the control exposure for 3 and 5 h at a magnetic field of 0.4 mT. Different solution (cellular suspension, medium and sterile distilled water) exposure to magnetic fields has different bioeffect on M. purpureus SKY219. The appropriate magnetic field treatment could increase the efficiency of red and yellow pigment production.


Monascus purpureus Pigments Liquid-state fermentation ELF-MF 



We are grateful to Anita Acai for comments and revision of this manuscript. This work was supported by Jiangsu Provincial Key Lab of Physics Processing of Agricultural Products (Grant number: JAPP2010-3) and Yangtze University for Ph.D. Studies (03000282).

Conflict of interest


Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.


  1. 1.
    Nimnoi P, Lumyong S (2011) Improving solid-state fermentation of Monascus purpureus on agricultural products for pigment production. Food Bioprocess Technol 4:1384–1390CrossRefGoogle Scholar
  2. 2.
    Dufosse L (2006) Microbial production of food grade pigment. Food Tech Biotech 44:313–321Google Scholar
  3. 3.
    Lin YL, Wang TH, Lee MH, Su NW (2008) Biologically active components and nutraceuticals in the Monascus-fermented rice: a review. Appl Microbiol Biotechnol 77:965–973CrossRefGoogle Scholar
  4. 4.
    Miyake T, Isato K, Nobuyuki N, Sammoto H (2008) Analysis of pigment compositions in various Monascus cultures. Food Sci Technol Res 14:194–197CrossRefGoogle Scholar
  5. 5.
    Mapari SA, Thranel U, Meyer AS (2010) Fungal polyketide azaphilone pigment as future natural food colourants? Trends Biotechnol 28:300–307CrossRefGoogle Scholar
  6. 6.
    Juzlova P, Martinkova L, Kern V (1996) Secondary metabolites of the fungus Monascus: a review. J Ind Microbiol Biot 16:163–170CrossRefGoogle Scholar
  7. 7.
    Vidyalakshmi R, Paranthaman R, Murugesh S, Singaravadivel K (2009) Stimulation of Monascus pigments by intervention of different nitrogen sources. Global J Biotechnol Biochem 4:25–28Google Scholar
  8. 8.
    Lin TF, Demain AL (1991) Effect of nutrition of Monascus sp. on formation of red pigments. Appl Microbiol Biotechnol 36:70–75CrossRefGoogle Scholar
  9. 9.
    Chen MH, Johns MR (1993) Effect of pH and nitrogen source on of pigment production by Monascus purpureus. Appl Microbiol Biotechnol 40:132–138CrossRefGoogle Scholar
  10. 10.
    Babitha S, Carvahlo JC, Soccol CR, Pandey A (2008) Effect of light on growth, pigment production and culture morphology of Monascus purpureus in solid-state fermentation. World J Microbiol Biotechnol 24:2671–2675CrossRefGoogle Scholar
  11. 11.
    Miyake T, Mori A, Kii T, Okuno T, Usui Y, Sato F, Sammoto H, Watanabe A, Kariyama M (2005) Light effects on cell development and secondary metabolism in Monascus. J Ind Microbiol Biotechnol 32:103–108CrossRefGoogle Scholar
  12. 12.
    Kim HJ, Kim HJ, Oh HJ, Shin CS (2002) Morphology control of Monascus cells and scale up of pigment fermentation. Process Biochem 38:649–655CrossRefGoogle Scholar
  13. 13.
    Alvarez DC, Perez VH, Justo OR, Alegre RM (2006) Effect of the extremely low frequency magnetic field on nisin production by Lactococcus lactis subsp. lactis using cheese whey permeate. Process Biochem 41:1967–1973CrossRefGoogle Scholar
  14. 14.
    Keklikci U, Akpolat V, Ozekinci S, Unlu K, Celik MS (2008) The effect of extremely low frequency magnetic field on the conjunctiva and goblet cells. Curr Eye Res 33:441–446CrossRefGoogle Scholar
  15. 15.
    Canli O, Erdal S, Taskin M, Kurbanoglu EB (2011) Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis. Toxicol Ind Health 27:35–39CrossRefGoogle Scholar
  16. 16.
    Motta MA, Muniz JBF, Schuler A, Motta M (2004) Static magnetic fields enhancement of Saccharomyces cerevisiae ethanolic fermentation. Biotechnol Prog 20:393–396CrossRefGoogle Scholar
  17. 17.
    Perez VH, Reyes AF, Justo OR, Alvarez DC, Alegre RM (2007) Bioreactor coupled with electromagnetic field generator: effects of extremely low frequency electromagnetic fields on ethanol production by Saccharomyces cerevisiae. Biotechnol Prog 23:1091–1094Google Scholar
  18. 18.
    Gao M, Zhang J, Feng H (2011) Extremely low-frequency magnetic field effects on metabolite of Aspergillus Niger. Bioelectromagnetics 32:73–78CrossRefGoogle Scholar
  19. 19.
    Deng G, Xia F, Wang J, Gao M (2012) Effect the low-frequency alternating magnetic field on biomass of Monascus solid-state fermentation (in Chinese). Transact Chin Soc Agricul Machi 43:128–132Google Scholar
  20. 20.
    Rosen MS, Rosen AD (1990) Magnetic field influence on paramecium motility. Life Sci 46:1509–1515CrossRefGoogle Scholar
  21. 21.
    Liu Y (2002) Electromagnetic biological effects. Beijing University of Posts and Telecommunications, BeijingGoogle Scholar
  22. 22.
    Prato FS, Kavaliers M, Thomas AW (2000) Extremely low frequency magnetic fields can either increase or decrease analgesia in the land snail depending on field and light conditions. Bioelectromagnetics 21:287–301CrossRefGoogle Scholar
  23. 23.
    Janać B, Selaković V, Rauš S, Radenović L, Zrnić M, Prolić Z (2012) Temporal patterns of extremely low frequency magnetic field-induced motor behavior changes in Mongolian gerbils of different age. Int J Radiat Biol 88:359–366CrossRefGoogle Scholar
  24. 24.
    Belyaev I (2011) Toxicity and SOS-response to ELF magnetic fields and nalidixic acid in E. coli cells. Mutat Res 722:56–61CrossRefGoogle Scholar
  25. 25.
    Sakurai T, Yoshimoto M, Koyama S, Miyakoshi J (2008) Exposure to extremely low frequency magnetic fields affects insulin-secreting cells. Bioelectromagnetics 29:118–124CrossRefGoogle Scholar
  26. 26.
    Betti L, Trebbi G, Fregola F, Zurla M, Mesirca P, Brizzi M, Borghini F (2011) Weak static and extremely low frequency magnetic fields affect in vitro pollen germination. Sci World J 11:875–890CrossRefGoogle Scholar
  27. 27.
    Nasher SH (2008) The effect of magnetic water on growth of chick-pea seeds. Eng Tech 26:16–20Google Scholar
  28. 28.
    Hirano M, Ohta A, Abe K (1998) Magnetic field effects on photosynthesis and growth of the cyanobacterium Spirulina platensis. J Ferment Bioeng 83:313–316CrossRefGoogle Scholar
  29. 29.
    Grewal HS, Maheshwari BL (2011) Magnetic treatment of irrigation water and snow pea and chickpea seeds enhances early growth and nutrient contents of seedlings. Bioelectromagnetics 32:58–65CrossRefGoogle Scholar
  30. 30.
    Moussa HR (2011) The impact of magnetic water application for improving common bean (Phaseolus vulgaris L.) production. New York Sci J 4:15–20Google Scholar
  31. 31.
    Alhassani DH, Amin GS (2012) Response of some productive traits of broiler chickens to magnetic water. Int J Poult Sci 11(2):158–160CrossRefGoogle Scholar
  32. 32.
    Pang XF, Deng B (2008) Investigation of changes in properties of water under the action of a magnetic field. Sci Chin Ser G Phys Mech Astron 51:1621–1632CrossRefGoogle Scholar
  33. 33.
    Ibrahim IH (2006) Biophysical properties of magnetized distilled water. Egypt J Sol 29:363–368Google Scholar
  34. 34.
    Chang KT, Weng CI (2006) The effect of an external magnetic field on the structure of liquid water using molecular dynamics simulation. J Appl Phys 100:1–6Google Scholar
  35. 35.
    Toledo EJL, Ramalho TC, Magriotis ZM (2008) Influence of magnetic field on physical-chemical properties of the liquid water: insights from experimental and theoretical models. J Mol Struct 888:409–415CrossRefGoogle Scholar
  36. 36.
    Pang XF (2006) The conductivity properties of protons in ice and mechanism of magnetization of liquid water. Eur Phys J B 49:5–23CrossRefGoogle Scholar
  37. 37.
    Moon JD, Chung HS (2000) Acceleration of germination of tomato seed by applying AC electric and magnetic field. J Electrost 48:103–114CrossRefGoogle Scholar
  38. 38.
    Alkhazan MMK, Saddiq AAN (2010) The effect of magnetic field on the physical, chemical and microbiological properties of the lake water in Saudi Arabia. J Evol Biol Res 2:7–14Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.College of Animal ScienceYangtze UniversityJingzhouChina
  2. 2.College of Life ScienceYangtze UniversityJingzhouChina

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