European Food Research and Technology

, Volume 238, Issue 1, pp 105–112 | Cite as

Physicochemical properties of calcium-fortified soymilk with microencapsulated and chelated calcium salt

Original Paper


In the current research, soymilk was fortified with 2,000 mg L−1 calcium (Ca) through direct addition and microencapsulated Ca. There have been no reports regarding Ca-fortification of soymilk by W/O/W double-emulsion method. In order to inhibit Ca–protein interaction and improve soymilk stability, different concentrations (0, 10, 15, 22, 25 and 30 g L−1) of potassium citrate were added to soymilk samples as a metal-chelating agent (CA). Adding CA caused an undesirable aftertaste. Thus, in order to solve this problem, Ca was microencapsulated using water-in-oil-in-water (W/O/W) double-emulsion method, and coacervates were formed by means of gelatin–agar electrostatic interaction. Physicochemical properties of pasteurized soymilk including pH, phase separation index, sedimentation, viscosity, conductivity and color parameters (L, a, b and ΔE) were assayed over storage time. CA addition led to reduction in phase separation index, sedimentation, viscosity and increase in conductivity. However, microcapsule addition showed no phase separation so that soymilk stabilization was achieved; still, viscosity, conductivity and ΔE parameters were increased by Ca microcapsule addition. Microencapsulation was a proper Ca-fortification method in soymilk regarding higher stability of the final product over pasteurization shelf life.


Soymilk Calcium Microencapsulation W/O/W double emulsion Chelating agent 



This study was supported by Isfahan University of Technology (IUT). The authors would like to thank Mr. Bahman Bahrami for his good technical guidance and Isfahan Soymilk Co. for the soybean supply.

Conflict of interest


Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.


  1. 1.
    Rosenthal A, Deliza R, Cabral LMC, Cabral LC, Farias CAA, Domingues AM (2003) Effect of enzymatic treatment and filtration on sensory characteristics and physical stability of soymilk. Food Control 14:187–192CrossRefGoogle Scholar
  2. 2.
    Pathomrungsiyounggul P, Grandison AS, Lewis MJ (2010) Effect of calcium carbonate, calcium citrate, tricalcium phosphate, calcium gluconate and calcium lactate on some physicochemical properties of soymilk. Int J Food Sci Technol 45:2234–2240CrossRefGoogle Scholar
  3. 3.
    Bendich A (2001) Calcium supplementation and iron status of females. Nutrition 17:46–51CrossRefGoogle Scholar
  4. 4.
    Theobald HE (2005) Dietary calcium and health. Nutr Bull 30:237–277CrossRefGoogle Scholar
  5. 5.
    Chaiwanon P, Puwastien P, Nitithamyong A, Sirichakwal PP (2000) Calcium fortification in soybean milk and In Vitro bioavailability. J Food Comp Anal 13:319–327CrossRefGoogle Scholar
  6. 6.
    Weingartner KE, Nelson AI, Erdman JW (1983) Effects of calcium addition on stability and sensory properties of soy beverage. J Food Sci 48:256–257CrossRefGoogle Scholar
  7. 7.
    Heaney RP, Dowell MS, Rafferty K, Bierman J (2000) Bioavailability of the calcium in fortified soy imitation milk, with some observations on method. Am J Clin Nutr 71:1166–1169Google Scholar
  8. 8.
    Saio K, Koyama E, Watanabe T (1967) Protein-calcium-phytic acid relationship in soybean. 1. Effect of calcium and phosphorus on solubility characteristics of soybean meal protein. Agr Biol Chem 31:1195–1200CrossRefGoogle Scholar
  9. 9.
    Sanguansri L, Augustin MA (2010) In: Smith J, Charter E (eds) Functional food product development. Wiley-Blackwell, NewfoundlandGoogle Scholar
  10. 10.
    Lakkis JM, García AJM (2011) Microcapsules containing salts for food products. EP patent 2292102Google Scholar
  11. 11.
    Benichou A, Aserin A, Garti N (2004) Double emulsions stabilized with hybrids of natural polymers for entrapment and slow release of active matters. Adv Colloid Interface Sci 108–109:29–41CrossRefGoogle Scholar
  12. 12.
    Benichou A, Aserin A, Garti N (2007) W/O/W double emulsions stabilized with WPI–polysaccharide complexes. Colloids Surf A 294:20–32CrossRefGoogle Scholar
  13. 13.
    Khalid N, Kobayashi I, Neves MA, Uemura K, Nakajima M (2013) Preparation and characterization of water-in-oil emulsions loaded with high concentration of l-ascorbic acid. LWT Food Sci Technol 51(2):448–454CrossRefGoogle Scholar
  14. 14.
    Bonnet M, Cansell M, Berkaoui A, Ropers MH, Anton M, Leal-Calderon F (2009) Release rate profiles of magnesium from multiple W/O/W emulsions. Food Hydrocoll 23:92–101CrossRefGoogle Scholar
  15. 15.
    Jiménez-Alvarado R, Beristain CI, Medina-Torres L, Román-Guerrero A, Vernon-Carter EJ (2009) Ferrous bisglycinate content and release in W1/O/W2 multiple emulsions stabilized by protein–polysaccharide complexes. Food Hydrocoll 23:2425–2433CrossRefGoogle Scholar
  16. 16.
    Boral S, Bohidar HB (2010) Effect of ionic strength on surface-selective patch binding-induced phase separation and coacervation in similarly charged gelatin-agar molecular systems. J Physical Chem B 114:12027–12035CrossRefGoogle Scholar
  17. 17.
    Pearson D (1976) Chemical analysis of foods. Churchill Livingstone, LondonGoogle Scholar
  18. 18.
    Pathomrungsiyounggul P, Lewis MJ, Grandison ASm (2007) Effects of calcium chloride and sodium hexametaphosphate on certain chemical and physical properties of soymilk. J Food Sci 72:E428–E434CrossRefGoogle Scholar
  19. 19.
    AOAC (2005) Association of Official Analytical Chemists. AOAC Int, MarylandGoogle Scholar
  20. 20.
    Steel RGD, Torrie JH, Dickey D (1997) Principles and procedure of statistics: a biometrical approach. McGraw Hill BookCo Inc, New York, pp 352–358Google Scholar
  21. 21.
    Kwok KC, Niranjan K (1995) Review: effect of thermal processing on soymilk. Int J Food Sci Technol 30:263–295CrossRefGoogle Scholar
  22. 22.
    Yazici F, Alvarez VB, Mangino ME, Hansen PMT (1997) Formulation and processing of a heat stable calcium-fortified soy milk. J Food Sci 62:535–538CrossRefGoogle Scholar
  23. 23.
    Pathomrungsiyounggul P, Lewis MJ, Grandison AS (2010) Effects of calcium-chelating agents and pasteurisation on certain properties of calcium-fortified soy milk. Food Chem 118:808–814CrossRefGoogle Scholar
  24. 24.
    Achouri A, Boye JI, Zamani Y (2007) Changes in soymilk quality as a function of composition and storage. J Food Qual 30:731–744CrossRefGoogle Scholar
  25. 25.
    Bai Y, Wilson LA, Glatz BA (1998) Quality of commercial shelf-stable soymilk products. J Food Prot 61:1161–1164Google Scholar
  26. 26.
    Molina MAI, Wagner JR (1999) The effects of divalent cations in the presence of phosphate, citrate and chloride on the aggregation of soy protein isolate. Food Res Int 32:135–143CrossRefGoogle Scholar
  27. 27.
    Cruz N, Capellas M, Hernández M, Trujillo AJ, Guamis B, Ferragut V (2007) Ultra high pressure homogenization of soymilk: microbiological, physicochemical and microstructural characteristics. Food Res Int 40:725–732CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Department of Food Science and Technology, College of AgricultureIsfahan University of TechnologyEsfahānIran

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