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Modelling of acrylamide formation in thermally treated red bell peppers (Capsicum annuum L.)

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Abstract

In this study, modelling of time/temperature-depending acrylamide formation in red bell peppers was performed. The data collected were analysed using a central composite design and response surface modelling, to optimize the thermal treatment (temperature and time of heating) of red bell pepper, in order to find the condition of lower acrylamide production maintaining sufficient status of processed vegetable. Using this approach, the acrylamide content in studied samples was in the range of 47 ± 12.03 and 10,616 ± 189.47 ng/g at temperature/time condition between 170 and 240 °C, respectively, 4–46 min. The calculated equation of acrylamide formation in grilled red bell pepper revealed that the minimum acrylamide formation is at the lowest exposure times, between 4 and 15 min. Also, the lower the cooking temperature is, the lower the acrylamide formation is. Moreover, the presented model enables the prediction of the acrylamide level at various temperature/time conditions of red bell pepper thermal treatment.

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References

  1. INFOSAN (2005) Information note no. 2. Acrylamide in food is a potential health hazard. Geneva, Switzerland

  2. IARC (1994) Some industrial chemicals. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 60, IARC Lyon, France

  3. Stadler RH, Blank I, Varga N, Robert F, Hau J, Guy PA, Robert MC, Riediker S (2002) Acrylamide from Maillard reaction products. Nature 419:449–450

    Article  CAS  Google Scholar 

  4. Mottram DS, Wedzicha BL, Dodson A (2002) Acrylamide is formed in the Maillard reaction. Nature 419:448–449

    Article  CAS  Google Scholar 

  5. Becalski A, Lau BPY, Lewis D, Seaman SW (2003) Acrylamide in foods: occurrence, sources and modeling. J Agric Food Chem 51:802–808

    Article  CAS  Google Scholar 

  6. Zyzak DV, Sanders RA, Stojanovic M, Tallmadge DH, Eberhart BL, Ewald DK, Gruber DC, Morsch TR, Strothers MA, Rizzi GP, Villagran MD (2003) Acrylamide formation mechanism in heated foods. J Agr Food Chem 51:4782–4787

    Article  CAS  Google Scholar 

  7. Yaylayan VA, Stadler RH (2005) Acrylamide formation in food: a mechanistic perspective. J AOAC Int 88:262–267

    CAS  Google Scholar 

  8. Medeiros Vinci R, Frédéric Mestdagh F, De Meulenaer B (2012) Acrylamide formation in fried potato products—Present and future, a critical review on mitigation strategies. Food Chem 133:1138–1154

    Article  CAS  Google Scholar 

  9. Rydberg P, Eriksson S, Tareke E, Karlsson P, Ehrenberg L, Törnqvist M (2003) Investigations of factors that influence the acrylamide content of heated foodstuffs. J Agric Food Chem 51:7012–7018

    Article  CAS  Google Scholar 

  10. Brathen E, Knutsen SH (2005) Effect of temperature and time on the formation of acrylamide in starch-based and cereal model systems, flat breads and bread. Food Chem 92:693–700

    Article  CAS  Google Scholar 

  11. Claeys WL, De Vleeschouwer K, Hendrickx ME (2005) Quantifying the formation of carcinogens during food processing: acrylamide. Trends Food Sci Tech 16:181–193

    Article  CAS  Google Scholar 

  12. Pedreschi F, Moyano P, Kaack K, Granby K (2005) Color changes and acrylamide formation in fried potato slices. Food Res Int 38:1–9

    Article  CAS  Google Scholar 

  13. Williams JSE (2005) Influence of variety and processing conditions on acrylamide levels in fried potato crisps. Food Chem 90:875–881

    Article  CAS  Google Scholar 

  14. Ciesarová Z, Kiss E, Kolek E (2006) Study of factors affecting acrylamide levels in model systems. Czech J Food Sci 24:133–137

    Google Scholar 

  15. Taubert D, Harlfinger S, Henkes L, Berkels R, Schömig E (2004) Influence of processing parameters on acrylamide formation during frying of potatoes. J Agric Food Chem 52(9):2735–2739

    Article  CAS  Google Scholar 

  16. Becalski A, Lau BPY, Lewis D, Seaman SW, Hayward S, Sahagian M, Ramesh M, Leclerc Y (2004) Acrylamide in french fries: influence of free amino acids and sugars. J Agric Food Chem 52:3801–3806

    Article  CAS  Google Scholar 

  17. Petersson EV, Rosén J, Turner C, Danielsson R, Hellenäs KE (2006) Critical factors and pitfalls affecting the extraction of acrylamide from foods: an optimisation study. Anal Chim Acta 557:287–295

    Article  CAS  Google Scholar 

  18. Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M (2002) Analysis of acrylamide, acarcinogen formed in heated foodstuffs. J Agric Food Chem 50:4998–5006

    Article  CAS  Google Scholar 

  19. Kaplan O, Kaya G, Ozcan C, Ince M, Yaman M (2009) Acrylamide concentrations in grilled foodstuffs of Turkish kitchen by high performance liquid chromatography-mass spectrometry. Microchem J 93:173–179

    Article  CAS  Google Scholar 

  20. Leung KS, Lin A, Tsang CK, Yeung STK (2003) Acrylamide in Asian foods in Hong Kong. Food Addit Contam 20:1105–1113

    Article  CAS  Google Scholar 

  21. Croft M, Tong P, Fuentes D, Hambridge T (2004) Australian survey of acrylamide in carbohydrate-based foods. Food Addit Contam 21:721–736

    Article  CAS  Google Scholar 

  22. Ciesarová Z, Balasová V, Kiss E, Kolek E, Šimko P, Kováč M (2004) Comparison of two methods for acrylamide determination and dietary intake of acrylamide from potato crisps in Slovakia. Czech J Food Sci 22:251–254

    Google Scholar 

  23. Gianni S, Armando F, Gabriella M, Massimo R, Sauro V, Sergio A (2007) HPLC-MS validation of QualisaFoo® biosensor kit for cost-effective control of acrylamide levels in Italian coffee. Food Control 18:1267–1271

    Article  CAS  Google Scholar 

  24. Bermudo E, Moyano E, Puignou L, Galceran MT (2008) Liquid chromatography coupled to tandem mass spectrometry for the analysis of acrylamide in typical Spanish products. Talanta 76:389–394

    Article  CAS  Google Scholar 

  25. Amrein TM, Schonbachler B, Rohner F, Lukac H, Schneider H, Keiser A, Escher F, Amadò R (2004) Potential for acrylamide formation in potatoes: data from the 2003 harvest. Eur Food Res Technol 219:573–578

    Article  Google Scholar 

  26. Grob K, Biedermann M, Biedermann-Brem S, Noti A, Imhof D, Amrein TM, Pfefferle A, Bazzocco D (2003) French fries with less than 100 μg/kg acrylamide: collaboration between cooks and analysts. Eur Food Res Technol 217:185–194

    Article  CAS  Google Scholar 

  27. Lasekan O, Abbas K (2011) Investigation of the roasting conditions with minimal acrylamide generation in tropical almond (Terminalia catappa) nuts by response surface methodology. Food Chem 125:713–718

    Article  CAS  Google Scholar 

  28. Esbensen KH (2009) Multivariate data analysis in practice, 5th edn. CAMO Software AS, Oslo

    Google Scholar 

  29. Mottram DS, Low MY, Elmore JS (2006) In: Skog K, Alexander J (eds) Acrylamide and other hazardous compounds in heat treated foods. Woodhead Publishing, Cambridge

    Google Scholar 

  30. Timilsena YP, Khanal JS, Anal AK (2010) Acrylamide: thermally induced toxicant in foods and its control measures. J Food Sci Tech Nepal 6:19–30

    Google Scholar 

  31. Yang J, Powers JR, Boylston TD, Weller KM (1999) Sugars and free amino acids in stored russet Burbank potatoes treated with CIPC and alternative sprout inhibitors. J Food Sci 64:592–596

    Article  CAS  Google Scholar 

  32. Salazar R, Arámbula-Villa G, Vázquez-Landaverde PA, Hidalgo FJ, Zamora R (2012) Mitigating effect of amaranth (Amarantus hypochondriacus) protein on acrylamide formation in foods. Food Chem 135:2293–2298

    Article  CAS  Google Scholar 

  33. Murkovic M (2004) Acrylamide in Austrian foods. J Biochem Biophys Methods 61:161–167

    Article  CAS  Google Scholar 

  34. Kim CT, Hwang ES, Lee HJ (2007) An improved LC-MS/MS method for the quantitation of acrylamide in processed foods. Food Chem 101:401–409

    Article  CAS  Google Scholar 

  35. Delgado-Andrade C, Morales FJ, Seiquer I, Navarro MP (2010) Maillard reaction products profile and intake from Spanish typical dishes. Food Res Int 43:1304–1311

    Article  CAS  Google Scholar 

  36. Senyuva HZ, Gokmen V (2005) Survey of acrylamide in Turkish foods by an in-house validated LC-MS method. Food Addit Contam 22:204–209

    Article  CAS  Google Scholar 

  37. Tateo F, Bononi M, Andreoli G (2007) Acrylamide levels in cooked rice, tomato sauces and some fast food on the Italian market. J Food Comp Anal 20:232–235

    Article  CAS  Google Scholar 

  38. Senyuva HZ, Gokmen V (2005) Study of acrylamide in coffee using an improved liquid chromatography mass spectrometry method: investigation of colour changes and acrylamide formation in coffee during roasting. Food Addit Contam 22:214–220

    Article  CAS  Google Scholar 

  39. Elmore JS, Koutsidis G, Dodson AT, Mottram DS, Wedzicha BL (2005) Measurement of acrylamide and its precursors in potato, wheat, and rye model systems. J Agric Food Chem 53:1286–1293

    Article  CAS  Google Scholar 

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Acknowledgments

The authors want to thank to the bilateral Slovak-Romanian cooperation supported by the Slovak Research and Development Agency under the projects SK-RO-0017-10 and SK-RO-0021-12. This work was also supported by the European Regional Development Fund through implementation of the project ITMS 26240120013 “Establishment of Hi-Tech Centre for Research of Formation, Elimination and Assessment of Contaminants in Food”.

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This article does not contain any studies with human or animal subjects.

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Correspondence to Gabriela Râpeanu.

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Oana Emilia Constantin and Kristina Kukurová have contributed equally to this study.

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Constantin, O.E., Kukurová, K., Neagu, C. et al. Modelling of acrylamide formation in thermally treated red bell peppers (Capsicum annuum L.) . Eur Food Res Technol 238, 149–156 (2014). https://doi.org/10.1007/s00217-013-2086-7

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  • DOI: https://doi.org/10.1007/s00217-013-2086-7

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