Abstract
The use of commercial roasted malts increases the content of the hop polyphenol xanthohumol (XN) in beer. This carrier effect is caused by high molecular melanoidins from roasted malt. Three roasting trials with different malts were performed in order to study the development of XN enrichment of wort and beer in laboratory and brewing trials. Different colour measurements, malt and flavour analysis, radical formation and antioxidative activity of selected samples were carried out. Furthermore, sensory tests of beers were conducted. During roasting the XN and isoxanthohumol enrichment in wort and beer rose with the roasting intensity of malt until it reached a maximum. The XN content in wort increased linearly with the colour of wort made from the malt samples. In PVPP-treated worts and in filtered beers, the XN content increased exponentially with the colour. After passing through a maximum, both the colour value and XN content in wort and beer decreased. Interestingly, the colour losses were more intensive than the losses of XN in worts and beers. The development of radical formation and the reducing power was linked during roasting. That means reducing groups of melanoidins are responsible for reducing power and prooxidative properties of malts. These functional groups of melanoidins are involved in the development of XN enrichment properties, because a linear correlation between these parameters was found. In conclusion, the roasting regimes showed potential for the development of special malt for the XN enrichment in beer- or malt-based beverages in late roasting stages. The use of this special malt brings more XN with less coloured malt in beer.
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References
Briggs DE (1998) Malt and malting, 1st edn. Blackie Academic & Professional, London
Jehle D, Lund MN, Øgendal LH et al (2011) Characterisation of a stable radical from dark roasted malt in wort and beer. Food Chem 125:380–387
Magalhaes PJ, Dostalek P, Cruz JM et al (2008) The impact of a xanthohumol-enriched hop product on the behavior of xanthohumol and isoxanthohumol in pale and dark beers. A pilot scale approach. J Inst Brew 114(3):246–256
Wunderlich S, Back W (2007) Brewing technological limits of xanthohumol enrichment in beer. In: Proceedings of the 31st Congress—European Brewery Convention, wunde1/1–wunde1/7
Wunderlich S, Zuercher A, Back W (2005) Enrichment of xanthohumol in the brewing process. Mol Nutr Food Res 49(9):874–881
Wunderlich S, Zuercher A, Back W (2012) Xanthohumol in brewing—impact of malt, xanthohumol dosage, wort and storage temperature. Brew Sci 65(1/2):7–15
Dorn C, Bataille F, Gaebele E et al (2010) Xanthohumol feeding does not impair organ function and homeostasis in mice. Food Chem Toxicol 48(7):1890–1897
Hanske L, Loh G, Sczesny S et al (2010) Recovery and metabolism of xanthohumol in germ-free and human microbiota-associated rats. Mol Nutr Food Res 54(10):1405–1413
Hussong R, Frank N, Knauft J et al (2005) A safety study of oral xanthohumol administration and its influence on fertility in Sprague Dawley rats. Mol Nutr Food Res 49(9):861–867
Walker CJ, Lence CF, Biendl M (2003) Studies on xanthohumol levels in stout/porter beer. Brauwelt 143(50):1709–1712
Biendl M, Methner F, Stettner G et al (2004) Brauversuche mit einem xanthohumolreichen Hopfenprodukt. Brauwelt 144:236–241
Magalhaes PJ, Almeida SM, Carvalho AM et al (2011) Influence of malt on the xanthohumol and isoxanthohumol behavior in pale and dark beers. A micro-scale approach. Food Res Int 44(1):351–359
Anonymous (1996) Spezialmalz für Spezialbiere. Brauwelt 5:213–216
Coghe S, Gheeraert B, Michiels A et al (2006) Development of Maillard reaction related characteristics during malt roasting. J Inst Brew 112(2):148–156
Pfenninger H (ed) (1993) Brautechnische Analysenmethoden, Band II. Methodensammlung d. Mitteleurop. Brautechn. Analysenkommission (MEBAK), 3rd edn. II. Selbstverlag der MEBAK, Freising
Clarke RJ (1987) Roasting and grinding. In: Clarke RJ, Macrae R (eds) Coffee: technology, vol 2. Elsevier Applied Science, London, pp 73–107
Herrmann M, Gastl M, Thiele F et al (2007) Malt volatile compounds. Brew Sci Monatsschrift fuer Brauwissenschaft 60:110–117
Eichhorn P, Miedaner H, Narziss L (1999) Untersuchungen zur Geschmacksstabilität des Bieres (Teil I). Monatsschrift fuer Brauwissenschaft 3(4):49–57
Wunderlich S, Biendl M, Zuercher A et al (2009) Reduced xanthohumol recovery after solid phase extraction of dark beers. Brew Sci 62:95–99
Kaneda H, Kobayashi N, Furusho S et al (1995) Reducing activity and flavor stability of beer. Tech Q Master Brew Assoc Am 32(2):90–94
Franz O, Back W (2001) DPPH-scavenging activity of beer and polyphenols measured by ESR. In: Proceedings of the 28th EBC Congress. Fachverlag Hans Carl, Nürnberg, Budapest, pp 621–630
Wurzbacher M, Back W (2007) ESR-Analytik zur Brauereiprozesskontrolle hinsichtlich der geschmacksstabilität. Brauwelt 22–23:594–598
Uchida M, Suga S, Ono M (1996) Improvement for oxidative flavor stability of beer—rapid prediction method for beer flavor stability by electron spin resonance spectroscopy. J Am Soc Brew Chem 54(4):205–211
Pfenninger H (ed) (1997) Brautechnische Analysenmethoden, Band I. Methodensammlung d. Mitteleurop. Brautechn. Analysenkommission (MEBAK), 3rd edn. I, Freising-Weihenstephan
Vandecan SMG, Daems N, Schouppe N et al (2011) Formation of flavor, color, and reducing power during the production process of dark specialty malts. J Am Soc Brew Chem 69(3):150–157
Kroh LW, Schulz A (2001) News on the Maillard reaction of oligomeric carbohydrates. A survey. Nahrung 45(3):160–163
Narziss L, Back W (2009) Die Bierbrauerei: Band II Die Technologie der Würzebereitung, 8th edn. Wiley-VCH Verlag GmbH & KGaA, Weinheim
Pazola Z (1987) The chemistry of cereal based beverage. In: Clarke RJ, Macrae R (eds) Coffee: related beverages, chap 3, vol 5. Elsevier Science, London, pp 59–104
Coghe S, D’Hollander H, Verachtert H et al (2005) Impact of dark specialty malts on the extract composition and wort fermentation. J Inst Brew 111(1):51–60
Horvath IS, Taherzadeh MJ, Niklasson C et al (2001) Effects of furfural on anaerobic continuous cultivation of Saccharomyces cerevisiae. Biotechnol Bioeng 75(5):540–549
Narziss L, Schuster K, Weinfurtner F (1999) Die Bierbrauerei: Band I Technologie der Malzbereitung, 7th edn. Ferdinand Enke Verlag, Stuttgart
Stevens JF, Taylor AW, Clawson JE et al (1999) Fate of xanthohumol and related prenylflavonoids from hops to beer. J Agric Food Chem 47(6):2421–2428
Lehtinen P, Kaukovirta-Norja A, Virtanen H et al (2005) Oxygen consumption during mashing-in. In: Proceedings of the 30th Congress—European Brewery Convention, pp 84/1–84/7
Spieleder E (2006) Systematische Untersuchungen von reduzierenden Substanzen im Malz und ihr Einfluss auf den Brauprozess. Dissertation, Technische Universität, München
Belitz H, Grosch W, Schieberle P (2009) Food Chemistry, 4th edn. Springer, Berlin
Cortés N, Kunz T, Suárez AF et al (2010) Development and correlation between the organic radical concentration in different malt types and oxidative beer stability. J Am Soc Brew Chem 68(2):107–113
Methner F, Kunz T, Kobayashi N (2008) Investigations on the behavior of organic radicals in barley and malt during the malting and mashing process by electron-spin-resonance spectroscopy. In: American Society of Brewing Chemists and Master Brewers Association of the Americas (ed) Proceedings of the World Brewing Congress 2008, presentation O-12
Adams A, Borrelli RC, Fogliano V et al (2005) Thermal degradation studies of food melanoidins. J Agric Food Chem 53:4136–4142
Kuntcheva MJ, Obretenov TD (1996) Isolation and characterization of melanoidins from beer. Zeitschrift für Lebensmitteluntersuchung und Forschung 202:238–243
O’Shaughnessy C, Chandra G, Fryer P et al (2003) Monitoring flavor development during the roasting of cereals. Tech Q Master Brew Assoc Am 40(2):98–107
Acknowledgments
We thank the company PROBAT for equipment and helpful assistance during the roasting experiments. Further, we thank M.Sc. Fehim Ali for conducting brewing experiments and Prof. Dr. Elke Arendt (University College Cork, Ireland) for the opportunity to do L*a*b measurements. We are also grateful to the malting company Weyermann® and Hopsteiner® for providing us with raw materials.
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Wunderlich, S., Wurzbacher, M. & Back, W. Roasting of malt and xanthohumol enrichment in beer. Eur Food Res Technol 237, 137–148 (2013). https://doi.org/10.1007/s00217-013-1970-5
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DOI: https://doi.org/10.1007/s00217-013-1970-5