A novel food-grade strain Lactobacillus plantarum 70810 producing β-galactosidase with high transgalactosylation activity was isolated from Chinese paocai. The galactooligosaccharides (GOS) were synthesized by using this enzyme with a maximum yield of 44.3 % (w/w) from 400 g/L lactose at 45 °C for 10 h. The β-galactosidase from this strain was purified to homogeneity by ammonium sulfate precipitation, anion exchange chromatography and gel filtration chromatography. It was a heterodimer arrangement of approximately 105 kDa composed of two subunits of 35 and 72 kDa. The optimal pH of the purified β-galactosidase was 8.0 for both o-nitrophenyl-β-d-galactopyranoside (oNPG) and lactose hydrolysis, and optimal temperature was 60 °C and 55 °C, respectively. Its K m and V max values for oNPG and lactose were 0.89 ± 0.05 mM, 194 ± 3.0 μmoL/min/mg protein, and 9.88 ± 0.16 mM, 15.88 ± 0.21 μmoL/min/mg protein, respectively. This enzyme was slightly inhibited by the hydrolysis products, that is, glucose and galactose. Since the β-galactosidase from L. plantarum 70810 exhibited higher transgalactosylation activity, strong affinity for lactose and low end-product inhibition, it was suggested to be a potential candidate for the synthesis of prebiotic GOS.
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This work was cofinanced by High-Tech Research and Development Program of China (No. 2011AA100903), National Natural Science Foundation of China (No. 31201422), Research Fund for the Doctoral Program of Higher Education of China, State Education Ministry (No. 20110097120028), Natural Science Foundation of Jiangsu Province (No. BK2011651), Youth Science and Technology Innovation Fund of Nanjing Agricultural Univerisity (No. KJ2010018) and was also supported by the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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Zhang, H., Li, W., Rui, X. et al. Lactobacillus plantarum 70810 from Chinese paocai as a potential source of β-galactosidase for prebiotic galactooligosaccharides synthesis. Eur Food Res Technol 236, 817–826 (2013). https://doi.org/10.1007/s00217-013-1938-5
- Lactobacillus plantarum 70810
- Chinese paocai