European Food Research and Technology

, Volume 236, Issue 4, pp 681–689 | Cite as

Angiotensin I-converting enzyme (ACE) inhibitory peptide derived from Tenebrio molitor (L.) larva protein hydrolysate

Original Paper

Abstract

Short peptides from different sources have been proven to be very efficient inhibitors of angiotensin I-converting enzyme (ACE), an enzyme with a major role in the regulation of blood pressure. In order to obtain ACE-inhibitory peptides from Tenebrio molitor (L.) larva, enzymatic hydrolysis was performed using Alcalase. The hydrolysate (DH 20 %) with the highest ACE inhibition was fractionated into four peaks according to their relative molecular weight by gel filtration on Sephadex G-15. The IC50 value of peak 2 (with the relative molecular weight 180–500 Da) was 0.23 mg/mL, while it was 0.39 mg/mL of hydrolysate before fractionation determined by reversed-phase high performance liquid chromatography (RP-HPLC). Antihypertensive activity of peak 2 was proven by single oral administration to spontaneously hypertensive rats (SHR). Multiple dose oral administration (100, 200, and 400 mg/kg body weight) to SHR led to a significant decrease in blood pressure for peak 2. The reduction of systolic blood pressure even reached 27 mm Hg at 4 h post-administration at a dose of 400 mg/kg body weight. Additionally, further separation and purification of the ACE-inhibitory peptides from peak 2 were performed using RP-HPLC on C18 column. Then a novel peptide Tyr–Ala–Asn was identified by tandem mass spectrometry with an IC50 value of 0.017 mg/mL. The result of research indicates that Tenebrio molitor (L.) larva protein may be a suitable candidate to prepare ACE-inhibitory peptides which could be used for food industry and nutraceuticals.

Keywords

ACE-inhibitory peptide Tenebrio molitor (L.) Larva Alcalase Hydrolysate Purification 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.School of Food and Biological EngineeringJiangsu UniversityZhenjiangChina
  2. 2.College of Life Sciences and Chemical EngineeringHuaiyin Institute of TechnologyHuaianChina

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