Abstract
Analytical methods for selenium (Se) speciation were developed using high-performance liquid chromatography (HPLC) coupled to either inductively coupled plasma mass spectrometry (ICP–MS) or electrospray ionization tandem mass spectrometry (ESI–MS/MS). Separations of selenomethionine (Se-Met) and selenocysteine (Se-(Cys)2) with favorable peak shape and resolution were obtained by both HPLC-ICP-MS and HPLC–ESI–MS/MS. Both methods achieved low limits of detection, high sensitivity and favorable stability. With HPLC–ESI–MS/MS, signal suppression was observed when complex matrix was co-eluted, but excellent structural characterization was still achieved. Thus, HPLC-ICP-MS is better for the detection of Se species, and HPLC–ESI–MS/MS is essential for molecular identification and confirmation. A water-soluble selenoprotein from purified M. anguillicaudatus muscle tissue was analyzed by the two complementary systems (HPLC-ICP-MS and HPLC–ESI–MS/MS) with high sensitivity and accuracy. The results demonstrated that Se-Met was the predominant selenoamino acid in the purified selenoprotein from M. anguillicaudatus muscle tissue, and the concentration of Se-Met in the selenoprotein was 6.280 mg/kg (dry mass). In addition, in HPLC-ICP-MS, an unknown Se-containing compound with similar polarity to Se-(Cys)2 was discovered. Using complementary data from HPLC–ESI–MS/MS, it was determined that this unknown Se-containing compound was not Se(Cys)2.
Similar content being viewed by others
Abbreviations
- HPLC–ICP–MS:
-
High-performance liquid chromatography-inductively coupled plasma mass spectrometry
- HPLC–ESI–MS/MS:
-
High-performance liquid chromatography–electrospray ionization tandem mass spectrometry
- MRM:
-
Multiple reaction monitoring
- Se-Met:
-
Selenomethionine
- Se-(Cys)2 :
-
Selenocysteine
References
Rayman MP (2000) Lancet 356:233–241
Spallholz JE, Palace VP, Reid TW (2004) Biochem Pharmacol 67:547–554
You L, Zhao M, Regenstein JM, Ren J (2010) Food Chem 120:810–816
Dong XZ, Xu HB, Huang KX, Liou Q, Zhou J (2002) Protein Expr Purif 26:235–242
US Department of Health and Human Services Food and Drug Administration website:http://www.fda.gov
National Academy of Sciences Institute of Medicine (IOM) website:http://www.iom.edu
Goenaga-Infante H, O’Connor G, Rayman M, Wahlen R, Spallholz J, Hearn R, Catterick T (2005) J Anal At Spectrom 20:864–870
Ayouni L, Barbier F, Imbert JL, Gauvrit JY, Lanteri P, Grenier-Loustalot MF (2006) Anal Bioanal Chem 385:1504
Mendez SP, Gonzalez EB, Medel AS (2000) J Anal Atom Spectrom 15:1109–1114
Sutton KL, Ponce de Leon C, Ackley KL, Sutton RMC, Stalcup AM, Caruso JA (2000) Analyst 125:231–234
Polatajko A, Banas B, Ruiz Encinar J, Spuznar J (2005) Anal Bioanal Chem 381:844
McSheey S, Yang W, Pannier F, Spuznar J, Lobinski R, Auger J, Potin-Gautier M (2000) Anal Chim Acta 421:157
Spuznar J (2005) Analyst 130:442–465
Peachey E, McCarthy N, Goenaga-Infante H (2008) J Anal At Spectrom 23(4):487–492
Goenaga-Infante H, Joel SP, Warburton E, Hopley C, Hearn R, Juliger S (2007) J Anal At Spectrom 22:888–896
McSheehy S, Pohl P, Szpunar J, Potin-Gautier M, Lobinski R (2001) J Anal At Spectrom 16:68–73
Ogra Y, Ishiwata K, Takayama H, Aimi N, Suzuki KT (2002) J Chromatogr B 767:301
Schaumlöffel D, Tholey A (2011) Anal Bioanal Chem 400:1645–1652
Urabe T, Tsugoshi T, Tanakaa W (2009) J Mass Spectrom 44:193–202
Di Marco VB, Raveane L, Dean A, Traldi P (2010) Rapid Commun Mass Spectrom 24:868–874
Warburton E, Goenaga-Infante H (2007) J Anal At Spectrom 22(4):370–376
Larsen EH (1998) Spectrochim Acta, Part B 53:253–265
Cairns WRL, Ebdon L, Hill SJ (1996) Fresenius J Anal Chem 355:202–208
Carr JE, Kwok K, Webster GK, Carnahan JW (2006) J Pharm Biomed Anal 40:42–50
Agnes GR, Horlick G (1994) Appl Spectrosc 48:655–661
Lindemann T, Hintelmann H (2002) Anal Bioanal Chem 372:486–490
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gong, L., Xu, Q., Lee, C. et al. Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC–ICP–MS and HPLC–ESI–MS/MS. Eur Food Res Technol 235, 169–176 (2012). https://doi.org/10.1007/s00217-012-1747-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00217-012-1747-2