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European Food Research and Technology

, Volume 234, Issue 4, pp 627–638 | Cite as

Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products

  • Natasa Golic
  • Ivana Strahinic
  • Amarela Terzic-Vidojevic
  • Jelena Begovic
  • Milica Nikolic
  • Maja Tolinacki
  • Ljubisa Topisirovic
Original Paper

Abstract

The diversity of 87 Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains, previously identified from different autochthonous dairy products, was investigated by phenotypic and genotypic approaches. The increased resolution obtained using phenotypic and genotypic characterization allowed the level of strain heterogeneity detection to be widened. Phenotypic diversity was evaluated by studying biochemical characteristics of technological interest, including antimicrobial and proteinase activities, resistance to nisin, aggregation ability, production of exopolysaccharides, acetoin and diacetyl, citrate utilization, and antibiotic susceptibility. Genotypic diversity was generally evaluated by PCR amplification of repetitive bacterial DNA element fingerprinting using the (GTG)5 primer [(GTG)5-PCR]. Moreover, in cases where strains were not discriminated by (GTG)5-PCR combined with phenotypic analysis, pulsed-field gel electrophoresis (PFGE) analysis was performed. The results indicate that L. plantarum/paraplantarum and L. paracasei natural isolates from artisanal dairy products are a gold mine in terms of diversity of strains and could be potentially interesting to dairy companies for the formulation of functional starter cultures in the production of innovative foods.

Keywords

Lactobacillus (GTG)5-PCR Microbial diversity Artisanal dairy products Natural isolates 

Abbreviation

MSL

Meters above sea level

Notes

Acknowledgments

This work was funded by the Ministry of Education and Science of the Republic of Serbia, grant No.: 173019. We are grateful to Prof. Dr Vladimir Stevanovic, Faculty of Biology, University of Belgrade, for detailed bio-geographical data of WBC regions from which dairy products were collected, to Dr Natasa Jokovic for clustering fingerprints in Statistica 7.0 for Windows (StatSoft Inc. USA), and to Myra (Macpherson) Poznanovic, official native English editor of the scientific journal Archives of Biological Sciences.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Natasa Golic
    • 1
  • Ivana Strahinic
    • 1
  • Amarela Terzic-Vidojevic
    • 1
  • Jelena Begovic
    • 1
  • Milica Nikolic
    • 1
  • Maja Tolinacki
    • 1
  • Ljubisa Topisirovic
    • 1
  1. 1.Institute of Molecular Genetics and Genetic EngineeringUniversity of BelgradeBelgradeSerbia

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