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Sensitive DNA-based allergen detection depends on food matrix and DNA isolation method

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Abstract

Performance of DNA-based methods used for allergen detection does not only depend on the real-time PCR system, but also on the DNA isolation method used. Comparison of different isolation methods showed that yield and purity of isolated DNA strongly depends on the isolation method deployed as well as on the composition of the food matrix (matrix effect). Detection of trace amounts of allergens requires efficient methods for DNA isolation which remove inhibitors and yield pure DNA. This is particularly important for quantitative analysis. Due to matrix effects, methods, which have the ability to circumvent these effects, are of great interest. Therefore, a sequence-specific method for isolation of target DNA based on magnetic particles (MCH) has been established and compared to other methods for isolation of total DNA.

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Acknowledgments

The authors want to thank Sven Hellwig, Katja Kirschbaum and Elisabeth Burgmaier-Thielert for their support. This work was funded by the BMBF (Bundesministerium für Bildung und Forschung), Germany (FKZ: 1734A08).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Life Sciences, University of Applied Sciences Albstadt-Sigmaringen, Anton-Günther-Straße 51, 72488, Sigmaringen, Germany

    Marion Kenk, Silvia Panter & Jörg Bergemann

  2. State Institute of Chemical and Veterinarian Analysis of Food Sigmaringen (CVUA Sigmaringen), Fidelis-Graf-Straße 1, 72488, Sigmaringen, Germany

    Gabriele Engler-Blum

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  1. Marion Kenk
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  2. Silvia Panter
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  3. Gabriele Engler-Blum
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  4. Jörg Bergemann
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Correspondence to Silvia Panter.

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Kenk, M., Panter, S., Engler-Blum, G. et al. Sensitive DNA-based allergen detection depends on food matrix and DNA isolation method. Eur Food Res Technol 234, 351–359 (2012). https://doi.org/10.1007/s00217-011-1639-x

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  • DOI: https://doi.org/10.1007/s00217-011-1639-x

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