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Rapid and simple method for the quantification of flavan-3-ols in wine

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An Erratum to this article was published on 28 December 2011

Abstract

A rapid and simple reverse-phase liquid chromatography coupled with fluorescence detection is proposed for the rapid simultaneous analysis of three monomeric flavanols and six procyanidins (i.e., five dimers and one trimer) in wine. Good resolution and low detection limits were obtained for (+)-catechin, (−)-epicatechin, (−)-epicatechin 3-O-gallate, dimers B1, B2, B3, B4 and B2-gallate and trimer C1. The method has been validated for linearity, precision and accuracy. Good performances in terms of selectivity and sensitivity have been also achieved. Moreover, new fluorimetric method has been developed in order to achieve the quantification of (−)-epicatechin 3-O-gallate and procyanidin B2-gallate by fluorescence detection.

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Acknowledgments

The authors wish to thank Amorim France (Eysines, France) and ANRT [Association Nationale pour la Recherche Technologique] (Paris, Cifre Grant No. 1290/2008) for their financial support in this research.

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Authors and Affiliations

  1. Unité de Recherche Oenologie, EA 4577, USC 1219 INRA, Faculté d’Oenologie, Institut des Sciences de la Vigne et du Vin, Université Bordeaux Segalen, 210 chemin de Leysotte, CS 50008, 33882, Villenave d’Ornon Cedex, France

    Maria A. Silva, Isabelle Ky, Michael Jourdes & Pierre-Louis Teissedre

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  1. Maria A. Silva
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  2. Isabelle Ky
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  3. Michael Jourdes
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  4. Pierre-Louis Teissedre
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Correspondence to Pierre-Louis Teissedre.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00217-011-1647-x.

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Silva, M.A., Ky, I., Jourdes, M. et al. Rapid and simple method for the quantification of flavan-3-ols in wine. Eur Food Res Technol 234, 361–365 (2012). https://doi.org/10.1007/s00217-011-1628-0

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  • DOI: https://doi.org/10.1007/s00217-011-1628-0

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