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Lipid composition and nutritional quality of intramuscular fat in Charneca-PDO beef

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Abstract

This paper characterizes the intramuscular fat from longissimus lumborum (LL, relatively red) and semitendinous (ST, relatively white) muscles of Charneca beef from young bulls reared according to the protected designation of origin (PDO) specifications. The content of total lipids, total cholesterol, α-tocopherol and β-carotene, as well as the fatty acid composition, including the isomeric distribution of conjugated linoleic acid (CLA), was assessed. Charneca young bulls (n = 10) were raised on a semi-extensive production system, in which animals fed pasture plus concentrate during 15 months. The ST muscle was leaner and had higher percentages of PUFA, in contrast to the LL muscle, which presented higher percentages of SFA and MUFA. Thus, the ST muscle had a higher PUFA/SFA ratio than the LL muscle, although the ratio values of both muscles were inside the recommended figures for the human diet. In contrast, the contents of CLA isomers, total cholesterol, α-tocopherol and β-carotene, as well as the n-6/n-3 ratio, were not influenced by muscle type, thus suggesting no carcass variation for these compounds. In both muscles, the n-6/n-3 ratios were slightly above the dietary guideline for human diet, and the contents of α-tocopherol were very high, indicating a good lipid stability of Charneca-PDO beef. Overall, the results suggest that intramuscular fat of Charneca-PDO beef has good human health–related parameters, with small carcass variation, since the PUFA/SFA and n-6/n-3 ratio values are inside or very close to the recommended figures for the human diet, and the content of α-tocopherol is very high.

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References

  1. 1.

    Scollan N, Hocquette JF, Nuernberg K, Dannenberger D, Richardson I, Moloney A (2006) Innovations in beef production systems that enhance the nutritional and health value of beef lipids and their relationship with meat quality. Meat Sci 74:17–33

    Article  CAS  Google Scholar 

  2. 2.

    EFSA European Food Safety Authority (2010) Scientific opinion on dietary reference values for fats, including saturated fatty acids, polyunsaturated fatty acids, monounsaturated fatty acids, trans fatty acids, and cholesterol. EFSA J 8(3):1–107

    Google Scholar 

  3. 3.

    Daley CA, Abbot A, Doyle PS, Nader GA, Larson S (2010) A review of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef. J Nutr 9:1–12

    Article  Google Scholar 

  4. 4.

    Griffin BA (2008) How relevant is the ratio of dietary n-6 to n-3 polyunsaturated fatty acids to cardiovascular disease risk? Evidence from the OPTILIP study. Curr Opin Lipidol 19:57–62

    Article  CAS  Google Scholar 

  5. 5.

    Gogus U, Smith C (2010) n-3 Omega fatty acids: a review of current knowledge. Int J Food Sci Tech 45:417–436

    Article  CAS  Google Scholar 

  6. 6.

    Kramer JKG, Sehat N, Dugan MR, Mossoba MM, Yurawecz MP, Roach JA, Eulitz K, Aalhus JL, Schaefer AL, Ku Y (1998) Distributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion higher-performance liquid chromatography. Lipids 33(6):549–558

    Article  CAS  Google Scholar 

  7. 7.

    Griinari JM, Bauman DE (1999) Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In: Yurawecz MP, Mossoba M, Kramer JK, Nelson G, Pariza MW (eds) Advances in conjugated linoleic acid research, vol 1. AOCS Press, Champaign, pp 180–200

    Google Scholar 

  8. 8.

    Benjamin S, Spener F (2009) Conjugated linoleic acids as functional food: an insight into their health benefits. Nutr Metab 6:36

    Article  Google Scholar 

  9. 9.

    French P, Stanton C, Lawless F, O’Riordan EG, Monahan FJ, Caffrey PJ, Moloney AP (2000) Fatty acid composition, including conjugated linoleic acid, of intramuscular fat from steers offered grazed grass, grass silage, or concentrate-based diets. J Anim Sci 78:2849–2855

    CAS  Google Scholar 

  10. 10.

    Barton L, Marounek M, Kudrna V, Bures D, Zahradkova R (2007) Growth performance and fatty acid profiles of intramuscular and subcutaneous fat from Limousin and Charolais heifers fed extruded linseed. Meat Sci 76:517–523

    Article  CAS  Google Scholar 

  11. 11.

    Chilliard Y, Glasser F, Ferlay A, Bernard L, Rouel J, Doreau M (2007) Diet, rumen biohydrogenation and nutritional quality of cow and goat milk fat. Eur J Lipid Sci Tech 109:828–855

    Article  CAS  Google Scholar 

  12. 12.

    Lourenço M, Van Ranst G, Vlaeminck B, De Smet S, Fievez V (2008) Influence of different dietary forages on the fatty acid composition of rumen digesta as well as ruminant meat and milk. Anim Feed Sci Tech 145:418–437

    Article  Google Scholar 

  13. 13.

    Descalzo AM, Sancho AM (2008) A review of natural antioxidants and their effects on oxidative status, odor and quality of fresh beef produced in Argentina. Meat Sci 79:423–436

    Article  CAS  Google Scholar 

  14. 14.

    Chizzolini R, Zanardi E, Dorigoni V, Ghidini S (1999) Calorific value and cholesterol content of normal and low-fat meat and meat products. Trends Food Sci Tech 10:119–128

    Article  CAS  Google Scholar 

  15. 15.

    American Heart Association (2008) Heart and stroke encyclopaedia. Dietary guidelines for healthy American global study. http://www.americanheart.org. Accessed 10 May 2011

  16. 16.

    Lee SH, Joo ST, Ryu YC (2010) Skeletal muscle fiber type and myofibrillar proteins in relation to meat quality. Meat Sci 86:166–170

    Article  CAS  Google Scholar 

  17. 17.

    Klont R, Brocks L, Eikelenboom G (1998) Muscle fibre type and meat quality. Meat Sci 49:S219–S229

    Article  Google Scholar 

  18. 18.

    Hocquette JF, Gondret F, Baéza E, Médale F, Jurie C, Pethick DW (2010) Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers. Animal 4(2):303–319

    Article  CAS  Google Scholar 

  19. 19.

    Costa P, Costa AF, Lopes PA, Alfaia CM, Bessa RJB, Roseiro LC, Prates JAM (2011) Fatty acid composition, cholesterol and α-tocopherol of Barrosã-PDO veal produced in farms located in lowlands, ridges and mountains. J Food Compos Anal 24:987–994

    Google Scholar 

  20. 20.

    IDRH, Instituto do Desenvolvimento Rural e Hidráulica (2007) Produtos Tradicionais com Nomes Protegidos—Apuramentos de 2005. Ministério da Agricultura, Desenvolvimento Rural e Pescas, Lisbon, pp 1–13

  21. 21.

    Vestergaard M, Oksbjerg N, Henckel P (2000) Influence of feeding intensity, grazing and finishing feeding on muscle fibre characteristics and meat colour of semitendinosus, longissimus dorsi and supraspinatus muscles of young bulls. Meat Sci 54:177–185

    Article  CAS  Google Scholar 

  22. 22.

    Alfaia CPM, Ribeiro VS, Lourenço MA, Quaresma MA, Martins SI, Portugal AP, Fontes CMGA, Bessa RJB, Castro MF, Prates JAM (2006) Fatty acid composition, conjugated linoleic acid isomers and cholesterol in beef from crossbred bullocks intensively produced and from Alentejana purebred bullocks reared according to Carnalentejana-PDO specifications. Meat Sci 72:425–436

    Article  CAS  Google Scholar 

  23. 23.

    Raes K, De Smet S, Demeyer D (2004) Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review. Anim Feed Sci Tech 113:199–221

    Article  CAS  Google Scholar 

  24. 24.

    Alves SP, Bessa RJB (2009) Comparison of two gas–liquid chromatographic columns for the analysis of fatty acids in ruminant meat. J Chromatogr A 1216:5130–5139

    Article  CAS  Google Scholar 

  25. 25.

    Kramer JKG, Hernandez M, Cruz-Hernandez C, Kraft J, Dugan MER (2008) Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2, 18:3 and CLA isomers of milk fat as demonstrated using Ag-ion SPE fractionation. Lipids 43:259–273

    Article  CAS  Google Scholar 

  26. 26.

    Prates JAM, Bessa RJB (2009) In: Nollet LML, Toldrá F (eds) Trans and n-3 fatty acids. Handbook of muscle foods analysis. Member of Francis & Taylor Group, CRC Press, Boca Raton (EUA)

    Google Scholar 

  27. 27.

    Agilent Technologies (2001) Agilent chemstation for LC 3D systems-understanding your spectra module user’s guide. Agilent Technologies Inc., Palo Alto

    Google Scholar 

  28. 28.

    Destaillats F, Angers P (2003) Directed sequential synthesis of conjugated linoleic acid isomers from d7, 9 to d12, 14. Eur J Lipid Sci Tech 105:3–8

    Article  CAS  Google Scholar 

  29. 29.

    Kraft J, Collomb M, Mockel P, Sieber R, Jahreis G (2003) Differences in CLA isomer distribution of cow’s milk lipids. Lipids 38:657–664

    Article  CAS  Google Scholar 

  30. 30.

    Cruz-Hernandez C, Kramer JKG, Kraft J, Santercole V, Or-Rashid M, Deng Z, Dugan MER, Delmonte P, Yurawecz MP (2006) Systematic analysis of trans and conjugated linoleic acids in the milk and meat of ruminants. In: Yurawecz MP, Kramer JKG, Gudmundsen O, Pariza MW, Banni S (eds) Advances in conjugated linoleic acid research, vol 3. AOCS Press, Champaign, pp 45–93

    Google Scholar 

  31. 31.

    Prates JAM, Quaresma MA, Bessa RJB, Fontes CMGA, Alfaia CPM (2006) Simultaneous HPLC quantification of total cholesterol, tocopherols and β-carotene in Barrosã-PDO veal. Food Chem 94:469–477

    Article  CAS  Google Scholar 

  32. 32.

    Food Advisory Committee (1990) Report on review of food labelling and advertising. Her Majesty’s Stationery Office, London

  33. 33.

    Enser M, Hallet B, Hewitt G, Fursey J, Wood D, Harrington G (1998) Fatty acid content and composition of UK beef and lamb muscle in relation to production system and implications for human nutrition. Meat Sci 49:329–341

    Article  CAS  Google Scholar 

  34. 34.

    Alfaia CPM, Alves SP, Martins SI, Costa ASH, Fontes CMGA, Lemos JPC, Bessa RJB, Prates JAM (2009) Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability. Meat Sci 114:939–946

    CAS  Google Scholar 

  35. 35.

    De la Fuente J, Díaz MT, Álvarez I, Oliver MA, Font i Furnols M, Sañudo C, Campo MM, Montossi F, Nute GR, Cañeque V (2009) Fatty acid and vitamin E composition of intramuscular fat in cattle reared in different production systems. Meat Sci 82:331–337

    Article  Google Scholar 

  36. 36.

    Vestergaard M, Therkildsen M, Henckel P, Jensen L, Andersen H, Sejrsen K (2000) Influence of feeding intensity, grazing and finishing feeding on meat and eating quality of young bulls and the relationship between muscle fibre characteristics, fibre fragmentation and meat tenderness. Meat Sci 54:187–195

    Article  CAS  Google Scholar 

  37. 37.

    Jenkins TC (1994) Regulation of lipid metabolism in the rumen. J Nutr 124:1372S–1376S

    CAS  Google Scholar 

  38. 38.

    Daniel Z, Wynn R, Salter A, Buttery P (2004) Different effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: the role of stearoyl-CoA desaturase. J Anim Sci 82:747–758

    CAS  Google Scholar 

  39. 39.

    Wood JD, Enser M, Fisher AV, Nute GR, Sheard PR, Richardson RI, Hughes SI, Wittington FM (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Sci 78:343–358

    Article  CAS  Google Scholar 

  40. 40.

    Realini CE, Duckett SK, Brito GW, Rizza MD, De Mattos D (2004) Effect of pasture vs. concentrate feeding with or without antioxidants on carcass characteristics, fatty acid composition, and quality of Uruguayan beef. Meat Sci 66:567–577

    Article  CAS  Google Scholar 

  41. 41.

    Bessa RJB, Santos-Silva J, Ribeiro JMR, Portugal AV (2000) Retículo-rumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers. Livest Prod Sci 63:201–211

    Article  Google Scholar 

  42. 42.

    Griinari JM, Corl BA, Lacy SH, Chouinard PY, Nurmela KVV, Bauman DE (2000) Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by D9-desaturase. J Nutr 130:2285–2291

    CAS  Google Scholar 

  43. 43.

    Costa P, Roseiro LC, Bessa RJB, Partidário A, Padilha M, Marques de Almeida J, Calkins C, Santos C (2008) Muscle fiber and fatty acid profiles of Mertolenga-PDO meat. Meat Sci 78:502–512

    Article  CAS  Google Scholar 

  44. 44.

    De Smet S, Raes K, Demeyer D (2004) Meat fatty acid composition as affected by fatness and genetic factors: a review. Anim Res 53:81–98

    Article  Google Scholar 

  45. 45.

    WHO (2003) Diet, nutrition and the prevention of chronic diseases. Report of a joint WHO/FAO expert consultation. WHO Technical Report Series no. 916. World Health Organization, Geneva

    Google Scholar 

  46. 46.

    Stanley JC, Elsom RL, Calder PC, Griffin BA, Harris WS, Jebb SA, Lovegrove JA, Moore CS, Riemersma RA, Sanders TAB (2007) UK food standards agency workshop report: the effects of the dietary n-6:n-3 fatty acid ratio on cardiovascular health. Br J Nutr 98:1305–1310

    Article  CAS  Google Scholar 

  47. 47.

    Brenna JT, Salem N, Sinclair AJ, Cunnane SC (2009) α-Linolenic acid supplementation and conversion to n-3 long-chain polyunsaturated fatty acids in humans. Prostag Leukotr Ess 80:85–91

    Article  CAS  Google Scholar 

  48. 48.

    Warren HE, Scollan ND, Enser M, Hughes SI, Richardson RI, Wood JD (2008) Effects of breed and a concentrate or grass silage diet on beef quality in cattle of 3 ages. I: animal performance, carcass quality and muscle fatty acid composition. Meat Sci 78:256–269

    Article  CAS  Google Scholar 

  49. 49.

    Alfaia CPM, Quaresma MA, Castro MF, Martins SI, Portugal AP, Fontes CMGA, Bessa RJB, Prates JAM (2006) Fatty acid composition, including isomeric profile of conjugated linoleic acid, and cholesterol in Mertolenga-PDO beef. J Sci Food Agric 86:2196–2205

    Article  CAS  Google Scholar 

  50. 50.

    Collomb M, Sieber R, Butikofer U (2004) CLA isomers in milk fat from 12 cows fed diets with high levels of unsaturated fatty acids. Lipids 39:355–364

    Article  CAS  Google Scholar 

  51. 51.

    Bauman DE, Baumgard LH, Corl BA, Griinari JM (2007) Biosynthesis of conjugated linoleic acid in ruminants. J Anim Sci 77:1–15

    Google Scholar 

  52. 52.

    Dannenberger D, Nuernberg K, Nuernberg G, Scollan N, Steinhart H, Ender K (2005) Effect of pasture vs. concentrate diets on CLA isomer distribution in different tissue lipids of beef cattle. Lipids 40:589–598

    Article  CAS  Google Scholar 

  53. 53.

    Nuernberger K, Nuernberg G, Ender K, Lorenz S, Winkler K, Rickert R, Steinhart H (2002) n-3 Fatty acids and conjugated linoleic acids of longissimus muscle in beef cattle. Eur J Lipid Sci Tech 104:463–471

    Article  Google Scholar 

  54. 54.

    Padre RG, Aricetti JA, Gomes STM, De Goes RHTB, Moreira FB, Prado IN, Visen JV, De Souza NE, Matsushita M (2007) Analysis of fatty acids in longissimus muscle of steers of different genetic breeds finished in pasture systems. Livest Sci 110:57–63

    Article  Google Scholar 

  55. 55.

    Yang A, Brewster MJ, Lanari MC, Tume RK (2002) Effect of vitamin E supplementation on α-tocopherol and β-carotene concentrations in tissues from pasture- and grain-fed cattle. Meat Sci 60:35–40

    Article  CAS  Google Scholar 

  56. 56.

    West J, Young OA, Agnew MP (1997) Levels of α-tocopherol in beef from New Zealand pastures. In: Proceedings of 43rd international congress of meat science and technology, ICoMST, Auckland

  57. 57.

    Lejeune A, Peng J, Le Boulengé E, Larondelle Y, Van Hove C (2000) Carotene content of Azolla and its variations during drying and storage treatments. Anim Feed Sci Tech 84:295–301

    Article  CAS  Google Scholar 

  58. 58.

    Yang A, Larsen TW, Tume RK (1992) Carotenoid and retinol concentrations in serum, adipose tissue and liver and carotenoid transport in sheep, goats and cattle. Aust J Agr Res 43:1807–1809

    Article  Google Scholar 

  59. 59.

    Yang A, McLennan SR, Armstrong J, Larsen TW, Shaw FD, Tume RK (1993) Effect of short-term grain feeding on bovine body-fat colour: a cautionary note. Aust J Agr Res 44:215–220

    Article  CAS  Google Scholar 

  60. 60.

    Food and Nutrition Board of Institute of Medicine (2001) Dietary reference intakes for vitamin C, vitamin E, selenium and carotenoids. National Academy Press, Washington, DC

  61. 61.

    Ip C, Singh M, Thompson HJ, Scimera JA (1994) Conjugated linoleic acid suppresses mammary carcinogenesis and proliferative activity of the mammary gland in the rat. Cancer Res 54:1212–1215

    CAS  Google Scholar 

  62. 62.

    Parrish FCJ, Wiegand BR, Beitz DC, Ahn DU, Du M, Trenkle AH (2003) Use of dietary CLA to improve composition and quality of animal-derived foods. In: Sébédio JL, Christie WW, Adlof R (eds) Advances in conjugated linoleic acid research, vol 2. AOCS Press, Champaign, pp 189–217

    Google Scholar 

  63. 63.

    Schmid A, Collomb M, Sieber R, Bee G (2006) Conjugated linoleic acid in meat and meat products: a review. Meat Sci 73:29–41

    Article  CAS  Google Scholar 

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Acknowledgments

Sampling assistance (Eng. Filomena Ferreira, ACBRP—Associação de Criadores de Bovinos de Raça Preta) and financial support from FCT grant (PTDV/CVT/2006/66114) and individual fellowships to J. M. Pestana (SFRH/PROTEC/2009/50138), A. S. H. Costa (SFRH/BD/2009/61068), P. Costa (SFRH/BPD/2008/46135), S. P. Alves (SFRH/BD/2007/37793) and S. V. Martins (SFRH/BPD/2009/63019) are acknowledged.

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Correspondence to José A. M. Prates.

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Pestana, J.M., Costa, A.S.H., Alfaia, C.M. et al. Lipid composition and nutritional quality of intramuscular fat in Charneca-PDO beef. Eur Food Res Technol 234, 187–196 (2012). https://doi.org/10.1007/s00217-011-1625-3

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Keywords

  • Beef
  • Fatty acids
  • CLA isomers
  • Total cholesterol
  • α-Tocopherol
  • β-Carotene