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Application of the simplex method for optimization of chromatographic analysis of biogenic amines in fish

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Abstract

The aim of this study was to optimize the separation of biogenic amines with ion-exchange high-performance liquid chromatography, using the modified simplex method. A modified chromatographic response function was used, and two methodological parameters were selected as simplex variables: the proportion of the organic solvent 2-propanol in the mobile phases, added as a new mobile phase, and the variation of the gradient curve at one specific step in the elution program. In optimum conditions the correct resolution and separation of the biogenic amines (tyramine, β-phenylethylamine, histamine, tryptamine, putrescine, cadaverine, agmatine, spermidine and spermine) were completed in 16 min, reducing the analysis time by 9 min compared with the original method. The regression coefficients were higher than 0.99 in all the amines, except for spermine (0.989). The detection limits varied between 0.06 mg/L, for histamine and agmatine, and 0.22 mg/L, for tryptamine, while retention time repeatability, determined as a relative standard deviation, was between 4.64% for histamine and 11.95% for tryptamine. The repeatability variation for the amine concentrates was found between 0.71% in cadaverine and 3.23% for tryptamine. Applying the optimized method to samples of refrigerated sardine and sea bream demonstrated the effectiveness of the method in these fish species.

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Acknowledgments

This research was supported under projects FAIR CT 98-3899 and the project AGL2008-04892, AGL2010-19515/ALI and CSD2007-00016 of the Programa Consolider Ingenio 2010 of the Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+I).

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  1. Department of Products: Fish and Meat Science and Technology, Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN-CSIC), (Formerly Instituto del Frío), Consejo Superior de Investigaciones Científicas (CSIC), C/José Antonio Novais, 10, Ciudad Universitaria, 28040, Madrid, Spain

    Juan Antonio Sánchez & Claudia Ruiz-Capillas

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  1. Juan Antonio Sánchez
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  2. Claudia Ruiz-Capillas
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Correspondence to Claudia Ruiz-Capillas.

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Sánchez, J.A., Ruiz-Capillas, C. Application of the simplex method for optimization of chromatographic analysis of biogenic amines in fish. Eur Food Res Technol 234, 285–294 (2012). https://doi.org/10.1007/s00217-011-1622-6

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  • DOI: https://doi.org/10.1007/s00217-011-1622-6

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