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A GC–MS-based metabonomic investigation of blood serum from irritable bowel syndrome patients undergoing intervention with acidified milk products

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Abstract

In the present study, the use of gas chromatography mass spectrometry (GC–MS)-based metabonomics to characterize blood serum in an intervention study of patients suffering from the common gastrointestinal disorder irritable bowel syndrome (IBS) was investigated. The patients included in the study consumed an acidified milk product with (n = 30) or without probiotics (n = 31) (Lactobacillus paracasei F19, Lactobacillus acidophilus LA-5 and Bifidobacterium lactis BB-12) for an 8-week period, and blood serum samples were collected before and after the intervention. Acidified milk is commonly used as a delivering vector for probiotics in commercial consumer settings. The serum samples were extracted and derivatized using N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA), and GC–MS analysis was carried out. Multivariate data analysis including principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and S-plot was applied on the obtained GC–MS data, which revealed higher serum lactate, glutamine, proline creatinine/creatine, and aspartic acid levels and lower serum glucose levels after the intervention period for both treatment groups. Consequently, the present study indicated an effect of acidified milk consumption on the plasma metabolite profile, which was independent of a concomitant intake of probiotics. In addition, the present study demonstrates that GC–MS is a useful analytical technique for metabonomics studies of blood serum.

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Acknowledgments

We acknowledge The Danish Dairy Council for funding the project “A nutrigenomic approach in the elucidation of basic molecular mechanisms for the action of probiotic bacteria”. In addition, the authors acknowledge support from the Danish National Research Foundation, Arla Foods and the Interdisciplinary Nanoscience Center (iNANO) at Aarhus University.

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Author notes

  1. Simon M. M. Pedersen

    Present address: Department of Ophthalmology, Aarhus University Hospital, Noerrebrogade 44, 8000, Aarhus C, Denmark

Authors and Affiliations

  1. Department of Food Science, Aarhus University, Blichers Allé 20, P.O. Box 50, 8830, Tjele, Denmark

    Simon M. M. Pedersen, Caroline Nebel & Hanne C. Bertram

  2. Center for Insoluble Protein Structures, Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Langelandsgade 140, 8000, Aarhus C, Denmark

    Simon M. M. Pedersen, Niels Chr. Nielsen & Anders Malmendal

  3. Arla Foods amba, P.O. Box 2400, 8260, Viby J, Denmark

    Henrik J. Andersen & Ulla Svensson

  4. KPL Good Food Practice AB, Dag Hammarskjölds vag 10 B 3tr, 751 83, Uppsala, Sweden

    Johan Olsson

  5. Department of Internal Medicine, Sahlgrenska University Hospital, Bruna Stråket 11, 413 45, Gothenburg, Sweden

    Magnus Simrén & Lena Öhman

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  1. Simon M. M. Pedersen
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Correspondence to Hanne C. Bertram.

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Pedersen, S.M.M., Nebel, C., Nielsen, N.C. et al. A GC–MS-based metabonomic investigation of blood serum from irritable bowel syndrome patients undergoing intervention with acidified milk products. Eur Food Res Technol 233, 1013–1021 (2011). https://doi.org/10.1007/s00217-011-1599-1

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  • DOI: https://doi.org/10.1007/s00217-011-1599-1

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