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The shock of vacuolar PrA on glycolytic flux, oxidative phosphorylation, and cell morphology by industrial Saccharomyces cerevisiae WZ65

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Proteinase A (PrA) is one of the most significant vacuolar proteinase in S. cerevisiae, and it plays an important role in S. cerevisiae physiology and metabolism, especially under unfavorable environment. In this study, the differences in pyruvate kinase (PYK) level under fructose-1,6-diphosphate (FDP) induction and ATP synthesis block among SC1 (the wild-type yeast that was industrial Saccharomyces cerevisiae WZ65), SC2 (PEP4 partial deletion) and SC3 (PEP4 complete deletion) were examined. Results showed that the induction caused by FDP clearly increased PYK expression no matter for which strain, but the increasing effect is more significant for SC2 (P < 0.05). The comparative results of intracellular ATP accumulation showed that the induction by FDP may be affected at the presence of PrA. The block experiment of ATP synthesis showed that PYK activities in PEP4-modified strains are lower than that of the wild type, but the intracellular ATP levels in the wild-type one are generally higher than the PEP4-modified strains after rotenone treatment (P < 0.01). This implies that the effect of PrA deficiency on intracellular ATP accumulation was much more pronounced than the effect of rotenone on oxidative phosphorylation. The cell morphology of three strains was comparatively examined by means of transmission electron microscopy (TEM). The PEP4-modified strains possessed more vacuoles, and cell structure were more integrated than the wild-type strain. Current data preliminarily indicated that the deletion of PEP4 gene in industrial S. cerevisiae WZ65 may not only affected PYK expression but also modulated the oxidative phosphorylation flux.

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Industrial Saccharomyces cerevisiae WZ65 (wild-type yeast)


Saccharomyces cerevisiae strain with PEP4 partial deletion


Saccharomyces cerevisiae strain with PEP4 complete deletion


Proteinase A


Proteinase B

PEP4 :

A kind of gene that encodes proteinase A










Pyruvate kinase


Adenosine triphosphate


Adenosine diphosphate


Reduced form of nicotinamide-adenine dinucleotide


Transmission electron microscopy


Glucose consumption rate, it is the D-value between the original glucose (g/50 mL) in the culturing medium and the residual glucose in the broth (g/50 mL) after certain culturing time divided by the original glucose in the culturing medium (g/50 mL)


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This work was financially supported by the National Hi-Tech Research and Development Program (863) of China (No. 2007AA10Z315).

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Correspondence to Qi-He Chen.

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Liu, XJ., Feng, Y., Fu, ML. et al. The shock of vacuolar PrA on glycolytic flux, oxidative phosphorylation, and cell morphology by industrial Saccharomyces cerevisiae WZ65. Eur Food Res Technol 233, 941–949 (2011).

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