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Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus in food

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Abstract

A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Staphylococcus aureus in food was developed and evaluated. Heat-stable nuclease (nuc) gene was the target sequence amplified by LAMP. LAMP includes primer design, amplification at 64 °C, macroscopic observation, and estimation of the results. The LAMP detection of S. aureus was sensitive up to 1.25 CFU per reaction tube, with a detection limit of 10.3 CFU per reaction tube in the artificial contamination test, and the experiment took less than 1 h. Sensitivity of the method in Staphylococcus aureus detection was 100%, with 97.93% specificity and a 98.5% match ratio when compared using Chinese National Standard. The LAMP method is superior to the Chinese National Standard method in terms of sensitivity, cost, testing time, and convenience.

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Acknowledgments

The work described in this paper was supported by the Natural Science Foundation of Hebei Province, China (Grant No. C2008000216).

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Authors and Affiliations

  1. College of Food Science and Technology, Agricultural University of HeBei, 071000, Baoding, People’s Republic of China

    Hong Yang, Xiaoyan Ma, Xianzhou Zhang, Yu Wang & Wei Zhang

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  1. Hong Yang
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  2. Xiaoyan Ma
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  3. Xianzhou Zhang
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  4. Yu Wang
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  5. Wei Zhang
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Correspondence to Wei Zhang.

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Yang, H., Ma, X., Zhang, X. et al. Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus in food. Eur Food Res Technol 232, 769–776 (2011). https://doi.org/10.1007/s00217-011-1442-8

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  • DOI: https://doi.org/10.1007/s00217-011-1442-8

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