ACE inhibitory and antihypertensive properties of apricot almond meal hydrolysate
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Apricot almond meal was hydrolyzed simultaneously with Neutrase and N120P proteases. The hydrolysate almond peptide (AP) was fractionated into three ranges of molecular weight (AP-I, >5 kDa; AP-II, 1–5 kDa; AP-III, <1 kDa) using an ultrafiltration membrane bioreactor system. The AP-III brought about a high angiotensin-I-converting enzyme (ACE) inhibitory activity with IC50 value of 0.138 mg mL−1 and the content of hydrophobic amino acid of AP-III was 50.08%. Lineweaver–Burk plots suggested that AP-III acts as a non-competitive inhibitor to inhibit ACE. And we evaluated the stability and in vivo antihypertensive activity of AP-III. Multiple dose oral administration (100 mg kg−1 body weight (BW), 400, 800 mg kg−1 BW) to spontaneously hypertensive rats led to a significant decrease in blood pressure for AP-III. Additionally, the gel filtration and RP-HPLC were used to separate ACE inhibitory peptides from the hydrolysate. The results suggested that the peptide derived from apricot almond protein may have potential applications as functional food.
KeywordsApricot almond meal Hydrolysate ACE inhibitory peptides Antihypertensive effect
This research was funded by Major Special Project of the Xinjiang Uygur Autonomous Region (200831108).
- 9.Miyoshi S, Ishikawa H, Kaneko T, Fukui F, Tanaka H, Maruyama S (1991) Structures and activity of Angiotensin-converting enzyme inhibitors in a α-zien hydrolysate. Agric Biol Chem 55:1313–1318Google Scholar
- 17.Tiwari RS, Venkatachalam M, Sharma GM, Su M, Roux KH, Sathe SK (2010) Effect of food matrix on amandin, almond (Prunus dulcis L.) major protein, immunorecognition and recovery. Food Sci Technol 43:675–683Google Scholar
- 18.Sheng X, Wang Z, Xu S (2008) Study on functional properties and structure of sugary almond protein. Sci Technol Food Industry 29:133–136Google Scholar
- 19.Wang S, Wen Z, Li H (2008) Analysis of the nutritional components and contents of sweet apricot kernel. Beijing Agric 9:13–15Google Scholar
- 28.Cheung HS, Wang FL, Ondetti MA, Sabo EF, Cushman DW (1980) Binding of peptide substrates and inhibitors of angiotensin converting enzyme. J Biol Chem 255(2):401–407Google Scholar