Skip to main content
SpringerLink
Log in

HPLC–PDA/ESI–MS/MS detection of polymethoxylated flavones in highly degraded citrus juice: a quality control case study

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

Highly degraded citrus fruit juices collected during a quality control investigation of the Italian Ministry of Agricultural Alimentary and Forestry Policies (MIPAAF) were analyzed for verifying their technological history. The analyzed samples were fermented black fluids stated as degraded first strength juices, in order to justify communitarian aids. Polymethoxylated flavones (PMFs) detection of solid phase extracted (SPE) purified samples provided clean chromatograms and good separation of compounds of interest using high performance liquid chromatography coupled with diode array and mass spectrometry HPLC–PDA/ESI–MS/MS. Since the PMFs concentration is elevate in peel flavedo, it is directly correlated with juice extraction strength. Results showed that all analyzed samples presented a PMFs concentration ranging from two to eight times first strength juices, not respecting the minimum quality requirements of the European regulatory framework.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Harborne JB (1994) Flavonoids: advances in research since 1986. Chapman and Hall, London

    Google Scholar 

  2. Miyake Y, Yamamoto K, Morimitsu Y, Osawa T (1997) Isolation of C-glucosylflavone from lemon peel and antioxidative activity of flavonoid compounds in lemon fruit. J Agric Food Chem 45:4619–4623

    Article  CAS  Google Scholar 

  3. Leuzzi U, Caristi C, Panzera V, Licandro G (2000) Flavonoids in pigmented orange juice and second-pressure extracts. J Agric Food Chem 48:5501–5506

    Article  CAS  Google Scholar 

  4. Ooghe WC, Ooghe SJ, Detavernier CM, Huyghebaert A (1994) Characterization of orange juice (Citrus sinensis) by polymethoxylated flavones. J Agric Food Chem 42:2191–2195

    Article  CAS  Google Scholar 

  5. Marini D, Balestrieri F (1995) Multivariate analysis of flavanone glycosides in Citrus juices. Ital J Food Sci 7:255–264

    CAS  Google Scholar 

  6. Dandana W, Jian W, Xuehui H, Yinga T, Kunyia N (2007) Identification of polymethoxylated flavones from green tangerine peel (Pericarpium Citri Reticulatae Viride) by chromatographic and spectroscopic techniques. J Pharm Biomed Anal 44:63–69

    Article  Google Scholar 

  7. Manthey JA, Buslic BS (2003) HPLC-MS analysis of methoxylated flavanones in orange oil residue. Proc Florida State Hortic Soc 116:410–413

    Google Scholar 

  8. Pupin AM, Dennis MJ, Toledo MCF (1998) Polymethoxylated flavones in Brazilian orange juice. Food Chem 63:13–518

    Article  Google Scholar 

  9. Mouly P, Gaydou EM, Auffray A (1998) Simultaneous separation of flavanone glycosides and polymethoxylated flavones in citrus juices using liquid chromatography. J Chrom A 800:171–179

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ministero delle Politiche Agricole Alimentari e Forestali, Laboratorio di Catania, Ispettorato centrale della tutela della qualità e repressione frodi dei prodotti agroalimentari (ICQRF), Via A. Volta 19, 95122, Catania, Italy

    Monica Scordino, Leonardo Sabatino, Pasqualino Traulo, Maria Gargano, Valentina Pantó & Giacomo Gagliano

Authors
  1. Monica Scordino
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leonardo Sabatino
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pasqualino Traulo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maria Gargano
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Valentina Pantó
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Giacomo Gagliano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Monica Scordino.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scordino, M., Sabatino, L., Traulo, P. et al. HPLC–PDA/ESI–MS/MS detection of polymethoxylated flavones in highly degraded citrus juice: a quality control case study. Eur Food Res Technol 232, 275–280 (2011). https://doi.org/10.1007/s00217-010-1386-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-010-1386-4

Keywords

Search

Navigation