Abstract
A total of 167 yeast and 136 lactic acid bacteria strains were isolated from spontaneously fermented wheat sourdoughs from two regions of Greece, namely Thessaly and Peloponnesus. Identification of the isolates exhibited dominance of Torulaspora delbrueckii with sporadic presence of Saccharomyces cerevisiae and, regarding the lactic acid bacteria, dominance of Lactobacillus sanfranciscensis in the sourdoughs from Thessaly and Lb. plantarum subsp. plantarum in the sourdoughs from Peloponnesus. The latter was accompanied by Pediococcus pentosaceus as secondary microbiota. None of the above mentioned strains exhibited amylolytic, lipolytic or proteolytic activities, and none of the lactic acid bacteria strains produced antimicrobial compounds.
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References
Arendt EK, Ryan LAM, Dal Bello F (2007) Impact of sourdough on the texture of bread. Food Microbiol 24:165–174
Gobbetti M, Rizzello CG, Di Cagno R, De Angelis M (2007) Sourdough lactobacilli and celiac disease. Food Microbiol 24:187–196
Corsetti A, Gobbetti M, De Marco B, Balestrieri F, Paoletti F, Russi L, Rossi J (2000) Combined effect of sourdough lactic acid bacteria and additives on bread firmness and staling. J Agr Food Chem 48:3044–3051
Hammes WP, Brandt MJ, Francis KL, Rosenheim J, Seitter MFH, Vogelmann A (2005) Microbial ecology of cereal fermentations. Trends Food Sci Tech 16:4–11
De Vuyst L, Neyssens P (2005) The sourdough microflora: biodiversity and metabolic interactions. Trends Food Sci Tech 16:43–56
Ehrmann MA, Vogel RF (2005) Molecular taxonomy and genetics of sourdough lactic acid bacteria. Trends Food Sci Tech 16:31–42
Paramithiotis S, Mueller MRA, Ehrmann MA, Tsakalidou E, Seiler H, Vogel R, Kalantzopoulos G (2000) Polyphasic identification of wild yeast strains isolated from Greek sourdoughs. Syst Appl Microbiol 23:156–164
De Vuyst L, Schrijvers V, Paramithiotis S, Hoste B, Vancanneyt M, Swings J, Kalantzopoulos G, Tsakalidou E, Messens W (2002) The biodiversity of lactic acid bacteria in Greek traditional wheat sourdoughs is reflected in both composition and metabolite formation. Appl Environ Microb 68:6059–6069
Barnett JA, Payne RW, Yarrow D (1990) Yeasts: characteristics and identification 2nd ed. University Press, Cambridge
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371
Kandler O, Weiss N (1986) Genus Lactobacillus Beijerinck 1901, 121AL. In: Sneath PA, Mair NS, Sharpe ME, Holts JG (eds) Bergey’s manual of systematic bacteriology, vol 4. The Williams & Wilkins Co., Baltimore, pp 1209–1234
Hammes WP, Hertel C (2006) The genera Lactobacillus and Carnobacterium. In: Dworkin M, Falkow S, Rosenberg E, Scheifer K-H, Stackebrandt E (eds) The prokaryotes, 3rd edn. Springer Science + Business Media, New York, pp 320–403
Paramithiotis S, Hondrodimou OL, Drosinos EH (2010) Development of the microbial community during spontaneous cauliflower fermentation. Food Res Int 43:1098–1103
Giraud E, Gosselin L, Marin B, Parada JL, Raimbault M (1993) Purification and characterization of an extracellular amylase from Lactobacillus plantarum strain A6. J Appl Bacteriol 75:276–282
Zotta T, Piraino P, Ricciardi A, McSweeney PLH, Parente E (2006) Proteolysis in model sourdough fermentations. J Agr Food Chem 54:2567–2574
Roecken W, Voysey PA (1995) Sourdough fermentation in bread making. J Appl Bacteriol 79:38–48
Succi M, Reale A, Andrighetto C, Lombardi A, Sorrentino E, Coppola R (2003) Presence of yeasts in Southern Italian sourdoughs from Triticum aestivum flour. FEMS Microbiol Lett 225:143–148
Gullo M, Romano AD, Pulvirenti A, Giudici P (2003) Candida humilis-dominant species in sourdoughs for the production of durum wheat bran flour bread. Int J Food Microbiol 80:55–59
Vernocchi P, Valmorri S, Dalai I, Torriani S, Gianotti A, Suzzi G, Guerzoni ME, Mastrocola D, Gardini F (2004) Characterization of the yeast population involved in the production of a typical Italian bread. J Food Sci 69:182–186
Vernocchi P, Valmorri S, Gatto V, Torriani S, Gianotti A, Suzzi G, Guerzoni ME, Gardini F (2004) A survey on yeast microbiota associated with an Italian traditional sweet-leavened baked good fermentation. Food Res Int 37:469–476
Loenner C, Ahrne S (1995) Lactobacillus (Baking). In: Hui YH, Khachatourians GG (eds) Food biotechnology. VCH Publishers Inc, Weinheim, pp 797–844
Rossi J (1996) The yeasts in sourdough. Adv Food Sci (CMTL) 5(6):201–211
Ottogalli G, Galli A, Foschino R (1996) Italian bakery products obtained with sourdough: characterization of the typical microflora. Adv Food Sci (CMTL) 5(6):131–144
Vogelmann SA, Seitter M, Singer U, Brandt MJ, Hertel C (2009) Adaptability of lactic acid bacteria and yeasts to sourdoughs prepared from cereals, pseudocereals and cassava and use of competitive strains as starters. Int J Food Microbiol 130:205–212
Brandt MJ, Hammes WP, Ganzle MG (2004) Effects of process parameters on growth and metabolism of Lactobacillus sanfranciscensis and Candida humilis during rye sourdough fermentation. Eur Food Res Technol 218:333–338
Gaenzle MG, Ehrmann M, Hammes WP (1998) Modelling of growth of Lactobacillus sanfranciscensis and Candida milleri in response to process parameters of sourdough fermentation. Appl Environ Microb 64:2616–2623
Valmorri S, Mortensen HD, Jespersen L, Corsetti A, Gardini F, Suzzi G, Arneborg N (2008) Variations of internal pH in typical Italian sourdough yeasts during co-fermentation with lactobacilli. LWT - Food Sci Technol 41:1610–1615
Lasztity R (1995) The chemistry of cereal proteins. 2nd ed. CRC Press, Boca Raton, FL
Almeida MJ, Pais CS (1996) Characterization of the yeast population from traditional corn and rye bread doughs. Lett Appl Microbiol 23:154–158
Almeida MJ, Pais CS (1996) Leavening ability and freeze tolerance of yeasts isolated from traditional corn and rye bread doughs. Appl Environ Microb 62:4401–4404
Ok T, Hashinaga F (1997) Identification of sugar-tolerant yeasts isolated from high sugar fermented vegetable extracts. J Gen Appl Microbiol 43:39–47
Hernandez-Lopez MJ, Prieto JA, Randez-Gil F (2003) Osmotolerance and leavening ability in sweet and frozen dough. Comparative analysis between Torulaspora delbrueckii and Saccharomyces cerevisiae baker’s yeast strains. Antonie van Leeuwenhoek 84:125–134
Alves-Araujo C, Almeida MJ, Sousa MJ, Leao C (2004) Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis. FEMS Microbiol Lett 240:7–14
Corsetti A, Settanni L, van Sinderen D, Felis GE, Dellaglio F, Gobbetti M (2005) Lactobacillus rossi sp.nov. isolated from wheat sourdough. Int J Syst Evol Micr 55:35–40
Vogel RF, Knorr R, Mueller MRA, Steudel U, Gaenzle MG, Ehrmann MA (1999) Non-dairy lactic fermentations: the cereal world. Antonie Van Leeuwenhoek 76:403–411
Gaenzle MG, Loponen J, Gobbetti M (2008) Proteolysis in sourdough fermentations: mechanisms and potential for improved bread quality. Trends Food Sci Tech 19:513–521
De Bruyne K, Camu N, De Vuyst L, Vandamme P (2009) Lactobacillus fabifermentans sp. nov. and Lactobacillus cacaonum sp. nov., isolated from Ghanaian cocoa fermentations. Int J Syst Evol Microbiol 59:7–12
Bringel F, Curk MC, Hubert JC (1996) Characterization of lactobacilli by Southern-type hybridization with a Lactobacillus plantarum pyrDFE probe. Int J Syst Bacteriol 46:588–594
Bringel F, Quenee P, Tailliez P (2001) Polyphasic investigation of the diversity within Lactobacillus plantarum related strains revealed two L. plantarum subgroups. Syst Appl Microbiol 24:561–571
Berthier F, Ehrlich SD (1998) Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region. FEMS Microbiol Lett 161:97–106
Torriani S, Felis GE, Dellaglio F (2001) Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers. Appl Environ Microbiol 67:3450–3454
Bringel F, Castioni A, Olukoya DK, Felis GE, Torriani S, Dellaglio F (2005) Lactobacillus plantarum subsp. argentoratensis subsp. nov. isolated from vegetable matrices. Int J Syst Evol Microbiol 55:1629–1634
Martinez-Anaya MA (1996) Enzymes and bread flavor. J Agr Food Chem 44:2469–2480
Pepe O, Villani F, Oliviero D, Greco T, Coppola S (2003) Effect of proteolytic starter cultures as leavening agents of pizza dough. Int J Food Microbiol 84:319–326
Martinez-Anaya MA, Rouzaud O (1997) Influence of wheat flour and Lactobacillus strains on the dynamics of by-products from amylolytic activities. Food Sci Technol Int 3:123–136
Calderon M, Loiseau G, Guyot JP (2001) Nutritional requirements and simplified cultivation medium to study growth and energetics of a sourdough lactic acid bacterium Lactobacillus fermentum Ogi E1 during heterolactic fermentation of starch. J Appl Microbiol 50:508–516
Giraud E, Brauman A, Keleke S, Lelong B, Raimbault M (1991) Isolation and physiological study of an amylolytic strain of Lactobacillus plantarum. Appl Microbiol Biot 36:379–383
Agati V, Guyot JP, Morion-Guyot J, Talamond P, Hounhouigan DJ (1998) Isolation and characterisation of new amylolytic strains of Lactobacillus fermentum from fermented maize doughs (mawe and ogi) from Benin. J Appl Microbiol 85:512–520
De Angelis M, Gobbetti M, Corsetti A (1999) Esterase and lipase activities of Lactobacillus sanfranciscensis strains used in sourdough fermentation. Ital J Food Sci 11:167–172
Gerez CL, Rollan GC, de Valdez GF (2006) Gluten breakdown by lactobacilli and pediococci strains isolated from sourdough. Lett Appl Microbiol 42:459–464
Zotta T, Ricciardi A, Parente E (2007) Enzymatic activities of lactic acid bacteria isolated from Cornetto di Matera sourdoughs. Int J Food Microbiol 115:165–172
Vermeulen N, Pavlovic M, Ehrmann MA, Gaenzle MG, Vogel RF (2005) Functional characterization of the proteolytic system of Lactobacillus sanfranciscensis DSM 20451T during growth in sourdough. Appl Environ Microb 71:6260–6266
Gobbetti M, Smacchi E, Corsetti A (1996) The proteolytic system of Lactobacillus sanfrancisco CB1: purification and characterization of a proteinase, a dipeptidase, and an aminopeptidase. Appl Environ Microb 62:3220–3226
Di Cagno R, De Angelis M, Lavermicocca P, De Vincenzi M, Giovannini C, Faccia M, Gobbetti M (2002) Proteolysis by sourdough lactic acid bacteria: effects on wheat flour protein fractions and gliadin peptides involved in human cereal intolerance. Appl Environ Microb 68:623–633
Larsen AG, Vogensen FK, Josephsen J (1993) Antimicrobial activity of lactic acid bacteria isolated from sour doughs: purification and characterization of bavaricin A, a bacteriocin produced by Lactobacillus bavaricus MI401. J Appl Bacteriol 75:113–122
Todorov S, Onno B, Sorokine O, Chobert JM, Ivanova I, Dousset X (1999) Detection and characterization of a novel antibacterial substance produced by Lactobacillus plantarum ST 31 isolated from sourdough. Int J Food Microbiol 48:167–177
Corsetti A, Gobbetti M, Smacchi E (1996) Antimicrobial activity of sourdough lactic acid bacteria: isolation of a bacteriocin-like inhibitory substance from Lactobacillus sanfrancisco C57. Food Microbiol 13:447–456
Gaenzle MG, Hoeltzel A, Walter J, Jung G, Hammes WP (2000) Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584. Appl Environ Microb 66:4325–4333
Mentes O, Ercan R, Akcelik M (2007) Inhibitor activities of two Lactobacillus strains, isolated from sourdough, against rope-forming Bacillus strains. Food Control 18:359–363
Messens W, De Vuyst L (2002) Inhibitory substances produced by Lactobacilli isolated from sourdoughs-a review. Int J Food Microbiol 72:31–43
Acknowledgments
The authors would like to thank Professor G.-J.E. Nychas, Laboratory of Microbiology and Biotechnology of Foods, for supplying Bacillus subtilis strain FMCC B-109.
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Paramithiotis, S., Tsiasiotou, S. & Drosinos, E.H. Comparative study of spontaneously fermented sourdoughs originating from two regions of Greece: Peloponnesus and Thessaly. Eur Food Res Technol 231, 883–890 (2010). https://doi.org/10.1007/s00217-010-1345-0
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DOI: https://doi.org/10.1007/s00217-010-1345-0