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European Food Research and Technology

, Volume 230, Issue 5, pp 723–729 | Cite as

Durum wheat cultivar traceability in PDO Altamura bread by analysis of DNA microsatellites

  • Antonella PasqualoneEmail author
  • Vittorio Alba
  • Giacomo Mangini
  • Antonio Blanco
  • Cinzia Montemurro
Original Paper

Abstract

Altamura bread is an Italian baking product that obtained the European mark of protected designation of origin (PDO). The varietal requirements of the official production protocol of this bread require it to be prepared from the durum wheat cultivars Appulo, Duilio, Arcangelo and Simeto (single or in combination, accounting for minimum 80%), and eventually other cultivars diffused in the production area. The aim of this work was to set up a microsatellite-based method for verifying the presence of the four required durum wheat cultivars in PDO Altamura bread, also in the presence of other cultivars up to 20%. Ten microsatellites were tested and the combination of the amplification profiles of four of them, characterised by high polymorphism and simple electrophoretic patterns, enabled to distinguish and identify breads from all the possible combinations of the cultivars required for PDO mark. The obtained amplicons were all in the range of molecular weight between 115 and 272 bp, and were analysed by capillary electrophoresis. The contribution of the single cultivars was detectable in the amplification profiles, enabling to verify their presence. The analysis was also effective in the case of additional cultivars.

Keywords

Durum wheat PDO Altamura bread Cultivar traceability DNA microsatellites Polymerase chain reaction 

Notes

Acknowledgments

The authors are indebted to “MIUR-Laboratorio di ricerca pubblico-privati”, Project AGROGEN, for financial support and gratefully acknowledge Panificio Digesù (Altamura, Italy) for accurate bread-making.

References

  1. 1.
    European Commission (2003) Commission Regulation 1291/2003/EC of 18/07/2003. Off J Eur Commun L181:12–19Google Scholar
  2. 2.
    European Council (1992) Council Regulation 2081/92 EEC of 14/07/1992. Off J Eur Commun L208:1–8Google Scholar
  3. 3.
    European Council (2006) Council Regulation (EC) 510/2006 of 20/03/2006. Off J Eur Commun L93:12–25Google Scholar
  4. 4.
    European Commission (2001) Council Regulation (EEC) 2081/92. Off J Eur Commun C156:10–15Google Scholar
  5. 5.
    Raffo A, Pasqualone A, Sinesio F, Paoletti F, Quaglia G, Simeone R (2003) Eur Food Res Technol 218:49–55CrossRefGoogle Scholar
  6. 6.
    Pasqualone A, Summo C, Bilancia MT, Caponio F (2007) J Food Sci 72:191–196CrossRefGoogle Scholar
  7. 7.
    Chiavaro E, Vittadini E, Musci M, Bianchi F, Curti E (2008) LWT 41:58–70CrossRefGoogle Scholar
  8. 8.
    Simeone R, Pasqualone A, Fares C, Blanco A (2001) Tec Molitoria 52:34–44Google Scholar
  9. 9.
    Pasqualone A, Blanco A, Fares C, Simeone R (2002) Tec Molitoria 53:770–779Google Scholar
  10. 10.
    Pasqualone A, Pellerano D, Caponio F, Simeone R (2005) Cereali: biotecnologie e processi innovativi. Una sfida per il mondo in cambiamento, 16–18 June 2005, Bari, Italy, pp 83-89. ISBN 2-85352-340-3Google Scholar
  11. 11.
    Tautz D (1989) Nucleic Acids Res 17:6463–6471CrossRefGoogle Scholar
  12. 12.
    Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Mol Gen Genet 246:327–333CrossRefGoogle Scholar
  13. 13.
    Röder MS, Wendehake K, Korzun V, Bredemeijer G, Laborie D, Bertrand L, Isaac P, Rendell S, Jackson J, Cooke RJ, Vosman B, Ganal MW (2002) Theor Appl Genet 106:67–73Google Scholar
  14. 14.
    Ma ZQ, Röder MS, Sorrells ME (1996) Genome 39:123–130CrossRefGoogle Scholar
  15. 15.
    Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Theor Appl Genet 94:557–563CrossRefGoogle Scholar
  16. 16.
    Pestsova E, Ganal MW, Röder MS (2000) Genome 43:689–697CrossRefGoogle Scholar
  17. 17.
    Agricultural Research Service of the US Department of Agriculture (2009) Graingenes 2.0: a database for Triticeae and Avena. http://wheat.pw.usda.gov/GG2/
  18. 18.
    Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK (2000) Theor Appl Genet 100:584–592Google Scholar
  19. 19.
    Vosman B, Cooke R, Ganal M, Peeters R, Isaac P, Bredemeijer G (2001) Acta Hortic 546:307–316Google Scholar
  20. 20.
    Cooke RJ, Bredemeijer GMM, Ganal MW, Peeters R, Isaac P, Rendell S, Jackson J, Röder MS, Korzun V, Wendehake K, Areshchenkova T, Dijcks M, Laborie D, Bertrand L, Vosman B (2003) Euphytica 132:331–341CrossRefGoogle Scholar
  21. 21.
    Perry DJ (2004) Theor Appl Genet 109:55–61CrossRefGoogle Scholar
  22. 22.
    Pasqualone A, Lotti C, Blanco A (1999) Eur Food Res Technol 210:144–147CrossRefGoogle Scholar
  23. 23.
    Pasqualone A, Montemurro C, Grinn-Gofron A, Sonnante G, Blanco A (2007) J Agric Food Chem 55:3312–3318CrossRefGoogle Scholar
  24. 24.
    Doyle JJ, Doyle JL (1990) Focus 12:13–15Google Scholar
  25. 25.
    Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier NH, Laroy P, Ganal MV (1998) Genetics 149:2007–2023Google Scholar
  26. 26.
    Song QJ, Shi JR, Singh S, Ficus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Theor Appl Genet 110:550–560CrossRefGoogle Scholar
  27. 27.
    Tilley M (2004) Cereal Chem 81:44–47CrossRefGoogle Scholar
  28. 28.
    Pasqualone A, Montemurro C, Sabetta W, Simeone R (2006) In: Proceedings of the 50th annual congress of Italian Society of Agriculture Genetics, 10–14 September 2006, Ischia, Italy, p B06. ISBN 88-900622-7-4Google Scholar
  29. 29.
    Pasqualone A, Montemurro C, Caponio F, Simeone R (2006) In: Proceedings of third AACC international symposium on Sourdough, 25–28 Oct 2006, Bari, Italy, p 12. ISBN 88-95240-00-6Google Scholar
  30. 30.
    Mangini G, Taranto F, Giove S, Gadaleta A, Blanco A (2008) In: Proceedings of the 52nd annual congress of Italian Society of Agriculture Genetics, 14–17 Sept 2008, Padova, Italy, p A05. ISBN 978-88-900622-8-5Google Scholar
  31. 31.
    Terzi V, Morcia C, Giovanardi D, D’Egidio MG, Stanca AM, Faccioli P (2004) Eur Food Res Technol 219:428–431CrossRefGoogle Scholar
  32. 32.
    GeneScan® Reference Guide (2000) Chapter 8 “Microsatellite Analysis”, Applied Biosystems USA. http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_040961.pdf

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Antonella Pasqualone
    • 1
    Email author
  • Vittorio Alba
    • 2
  • Giacomo Mangini
    • 2
  • Antonio Blanco
    • 2
  • Cinzia Montemurro
    • 2
  1. 1.Section of Agri-Food Industries, PROGESA DepartmentUniversity of BariBariItaly
  2. 2.Section of Genetics and Breeding, DIBCA DepartmentUniversity of BariBariItaly

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