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Determination of the antioxidant capacity: influence of the sample concentration on the measured values

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Abstract

The aim of the present analysis was to study the influence of the sample concentration on the measured antioxidant capacity, since such investigations are scarce but necessary to ensure the reproducibility of the results. Pure substances (ascorbic acid, gallic acid, Trolox®, uric acid) and food extracts (strawberry nectar, tomato extract, white tea) were analysed using seven common antioxidant capacity assays (three versions of Trolox equivalent antioxidant capacity, TEAC; ferric reducing antioxidant power, FRAP; photochemiluminescence, PCL; oxygen radical absorbance capacity, ORAC and total phenolics assay). For all applied pure substances and in most of the assays effects of the sample concentration on the measured antioxidant capacity were observed. Since it remains speculative how sample concentration does affect the measured antioxidant capacity exactly, it is strongly recommended to use at least three sample concentrations for analysis to detect and to discuss concentration-dependent effects.

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Acknowledgments

The technical support of I. Schmuck and H. Schmidt is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Nutrition, Friedrich Schiller University, Dornburger Str. 25-29, 07743, Jena, Germany

    Christin Hengst, Susanne Werner, Lars Müller, Kati Fröhlich & Volker Böhm

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  1. Christin Hengst
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  2. Susanne Werner
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  3. Lars Müller
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  4. Kati Fröhlich
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  5. Volker Böhm
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Correspondence to Volker Böhm.

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Hengst, C., Werner, S., Müller, L. et al. Determination of the antioxidant capacity: influence of the sample concentration on the measured values. Eur Food Res Technol 230, 249–254 (2009). https://doi.org/10.1007/s00217-009-1166-1

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  • DOI: https://doi.org/10.1007/s00217-009-1166-1

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