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Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products

European Food Research and Technology volume 230, pages 63–70 (2009)Cite this article

Abstract

A collection of 26 enterococci isolated from dairy and meat products were tested for antimicrobial and proteolytic activity. Enterococcus faecium and E. faecalis were the most frequent species among tested enterococci, and 11 isolates produced antimicrobial compounds. Results revealed that 10 out of 11 enterococci synthesized enterocins showing antimicrobial activity against food-born pathogen such as Listeria monocytogenes and Staphylococcus aureus. The broadest spectrum of antimicrobial activity was detected in E. faecalis BGPT1-10P and BGPT1-78. E. faecalis BG221 showed antimicrobial activity that was not related to production of enterocin, H2O2 or organic acid. Twenty-five enterococci showed strong or moderate proteolytic activity towards β-casein. Two isolates, BGPT1-10P and BGPT1-78, showed the most intense hydrolysis of αs1-, β-, κ-casein fractions, total casein as well as gelatin. Extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass of about 29 kDa. Bacteriocin production and proteinase activity of natural isolates of enterococci may be of technological interest in dairy and meat-fermented products.

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Acknowledgments

We are grateful to the Institute of Meat Hygiene and Technology, Belgrade for providing the Listeria monocytogenes ATCC 19111 strains. This work was funded by Ministry of Science and Technological Development of Republic of Serbia, grant No. 143036.

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Authors and Affiliations

  1. Laboratory for Molecular Genetics of Industrial Microorganisms, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia

    Katarina Veljovic, Djordje Fira, Amarela Terzic-Vidojevic & Ljubisa Topisirovic

  2. Department of Health Sciences, University of Jaen, 23071, Jaen, Spain

    Hikmate Abriouel & Antonio Galvez

Authors
  1. Katarina Veljovic
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  2. Djordje Fira
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  3. Amarela Terzic-Vidojevic
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  4. Hikmate Abriouel
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  5. Antonio Galvez
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  6. Ljubisa Topisirovic
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Correspondence to Ljubisa Topisirovic.

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Veljovic, K., Fira, D., Terzic-Vidojevic, A. et al. Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products. Eur Food Res Technol 230, 63–70 (2009). https://doi.org/10.1007/s00217-009-1137-6

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  • DOI: https://doi.org/10.1007/s00217-009-1137-6

Keywords

  • Enterococci
  • Antimicrobial activity
  • Enterocins
  • Proteolytic activity