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Aromatic DNA adducts in relation to dietary and other lifestyle factors in Spanish adults

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Abstract

The aim of this study was to assess the relationship between bulky DNA adducts in white blood cells (WBC) and lifestyle factors in a sample from the Spanish cohort of the European Prospective Investigation into Cancer and Nutrition (EPIC). 296 subjects aged between 35 and 64 years, from five regions, were included. Food intake was estimated with a computerized version of dietary history questionnaire. Daily intake of polycyclic aromatic hydrocarbons (PAH) was estimated using a database with information on food content of potential carcinogens. Data on lifestyle and health factors were collected and DNA adducts measured using the nuclease P1 32P-postlabelling technique. Geometric means of adducts were similar for men and women (4.11/109 and 3.94/109 nucleotides, respectively). Highest levels of adduct were observed in non-smokers and non-occupationally exposed. Meat intake, oils and fats were associated with higher levels of adducts, but all non-statistically significant. Higher intakes of calcium, sodium and phosphorus were associated with lower adducts levels. Summarising, our study shows that bulky adducts measured by 32P-postlabelling in DNA from WBC do not correlate with the usual diet of healthy Spanish adults. Although it has been proposed that diet be the main source of PAH in nonsmokers without occupational exposure, DNA adducts do not seem to be suitable biomarkers of dietary PAH in general population.

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Abbreviations

WBC:

White blood cells

EPIC:

European Prospective Investigation into Cancer and Nutrition

PAH:

Polycyclic aromatic hydrocarbons

B[a]P:

Benzo[a]pyrene

BPDE:

Benzopyrene-diol-epoxide

RAL:

Relative adduct labelling

DH:

Dietary history questionnaire

24-HDR:

24-h dietary recall

CI:

Confidence intervals

LRT:

Likelihood ratio test

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Acknowledgments

The project received financial support from the Health Research Fund (FIS, 02/0652) of the Spanish Ministry of Health, Red Temática de Investigación Cooperativa de Centros de Cáncer (C03/10) and the Environmental Cancer Risk, Nutrition and Individual Susceptibility (ECNIS, FP6, FOOD-CT-2005-513 943), and it was partially supported by the Associazione Italiana per la Ricerca sul Cancro (AIRC). All the authors have had no involvements that might raise the question of bias in the work reported or in the conclusions, implications or opinions stated.

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Authors and Affiliations

  1. Cancer Epidemiology Research Program, Unit of Infections and Cancer, Catalan Institute of Oncology (ICO), IDIBELL, Avd. Gran Vía, KM 2,7 s/n, L’Hospitalet de Llobregat, 08907, Barcelona, Spain

    Raquel Ibáñez

  2. Cancer Risk Factors Branch, Molecular Biology Laboratory, CSPO-Scientific Institute of Tuscany Region, Florence, Italy

    Marco Peluso, Armelle Munnia & Sara Piro

  3. Cancer Epidemiology Research Program, Unit of Nutrition, Environment and Cancer, Catalan Institute of Oncology (ICO), IDIBELL, Avd. Gran Vía, KM 2,7 s/n, L’Hospitalet de Llobregat, 08907, Barcelona, Spain

    Carlos A. González, Antonio Berenguer, Paula Jakszyn & Antonio Agudo

  4. Dirección de Salud de Guipúzcoa, CIBERESP, 20013, San Sebastián, Spain

    Pilar Amiano, Miren Dorronsoro & Nerea Larrañaga

  5. Consejería de Sanidad y Consumo, CIBERESP, 3008, Murcia, Spain

    M. J. Tormo, M. Dolores Chirlaque & Carmen Navarro

  6. Instituto de Salud Pública de Navarra, CIBERESP, 31003, Pamplona, Spain

    Eva Ardanaz & Aurelio Barricarte

  7. Escuela Andaluza de Salud Pública, CIBERESP, 18080, Granada, Spain

    Carmen Martínez & M. José Sánchez

  8. Consejería de Sanidad y Servicios Sociales de Asturias, 33001, Oviedo, Spain

    J. Ramón Quirós

Authors
  1. Raquel Ibáñez
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  2. Marco Peluso
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  3. Armelle Munnia
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  4. Sara Piro
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  5. Carlos A. González
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  6. Pilar Amiano
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  7. M. J. Tormo
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  8. Eva Ardanaz
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  10. Antonio Berenguer
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  11. M. Dolores Chirlaque
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  13. Paula Jakszyn
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  14. Nerea Larrañaga
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  15. Carmen Martínez
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  16. Carmen Navarro
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  17. J. Ramón Quirós
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  18. M. José Sánchez
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  19. Antonio Agudo
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Corresponding author

Correspondence to Raquel Ibáñez.

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Ibáñez, R., Peluso, M., Munnia, A. et al. Aromatic DNA adducts in relation to dietary and other lifestyle factors in Spanish adults. Eur Food Res Technol 229, 549–559 (2009). https://doi.org/10.1007/s00217-009-1089-x

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  • DOI: https://doi.org/10.1007/s00217-009-1089-x

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