Abstract
The expression of parvalbumin, a major allergenic protein of fish muscle, was determined in rapidly growing transgenic coho salmon (Oncorhynchus mykiss) containing the growth hormone (GH) gene construct OnMTGH1. Three different methods for parvalbumin analysis were used: (1) measurement of the mRNA concentration by real-time RT-PCR; (2) isoelectric focusing of sarcoplasmic proteins; (3) assessment of amount of heat-stable, Ca2+-binding sarcoplasmic proteins by measuring absorbance spectra of “stains-all”–protein complexes. Compared to non-transgenic coho salmon, no indication for enhanced expression of parvalbumin in transgenic fish was found either at the mRNA or at the protein level.
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References
Maclean N (2003) Trends Food Sci Technol 14:242–252
Guillen I, Berlanga J, Valenzuela CM, Morales A, Toledo J, Estrada MP, Puentes P, Hayes O, de la Fuente J (1999) Mar Biotechnol 1:2–14
Devlin RH, Yesaki TY, Donaldson EM, Swanson P, Cha W-K (1994) Nature 371:209–210
Devlin RH, Yesaki TJ, Donaldson EM, Du AJ, Hew C-L (1995) Can J Fish Aquat Sci 52:1376–1384
Devlin RH, Biagi CA, Yesaki TY (2004) Aquaculture 236:607–632
Hill JA, Kiessling A, Devlin RH (2000) Can J Fish Aquat Sci 57:939–950
Uh M, Khattra J, Devlin RH (2006) Transgenic Res 15:711–727
Raven PA, Devlin RH, Higgs DA (2006) Aquaculture 254:730–747
Rehbein H (2005) Eur Food Res Technol 220:625–632
Rehbein H, Etienne M, Jerome M, Hattula T, Knudsen LB, Jessen F, Luten JB, Bouquet W, Mackie IM, Ritchie AH, Martin R, Mendes R (1995) Food Chem 52:193–197
Girija N, Rehbein H (1988) Comp Biochem Physiol 91B:723–728
Layne E (1957) In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 3. Academic Press, New York, pp 450–451
Suresh AV, Sheehan RJ (1998) J Fish Biol 52:570–587
Bustin SA, Nolan T (2004) J Biomol Tech 15:155–166
Ellefsen S, Stenslokken K-O, Sandvik GK, Kristensen TA, Nilsson GE (2008) Anal Biochem 376:83–93
Rengmark AH, Slettan A, Lee WJ, Lie O, Lingaas F (2007) J Fish Biol 71(Supplement C):409–422
Rehbein H, van Lessen E (1989) Arch FischWiss 39:147–162
Caday CG, Lambooy PK, Steiner RF (1986) Biopolymers 25:1579–1595
Campbell KP, MacLennan DH, Jorgensen AO (1983) J Biol Chem 258:11267–11273
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The skilful technical assistance of Rainer Kündiger is gratefully acknowledged.
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Rehbein, H.W., Devlin, R.H. No evidence for enhanced parvalbumin concentration in light muscle of transgenic coho salmon (Oncorhynchus kisutch). Eur Food Res Technol 229, 579–584 (2009). https://doi.org/10.1007/s00217-009-1086-0
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DOI: https://doi.org/10.1007/s00217-009-1086-0