European Food Research and Technology

, Volume 229, Issue 4, pp 561–569

Development of a salmon protein hydrolysate that lowers blood pressure

  • H. Stephen Ewart
  • Dorothy Dennis
  • Michael Potvin
  • Christa Tiller
  • Lian-hua Fang
  • Ran Zhang
  • Xiao-ming Zhu
  • Jonathan M. Curtis
  • Sylvie Cloutier
  • Guanhua Du
  • Colin J. Barrow
Original Paper

DOI: 10.1007/s00217-009-1083-3

Cite this article as:
Ewart, H.S., Dennis, D., Potvin, M. et al. Eur Food Res Technol (2009) 229: 561. doi:10.1007/s00217-009-1083-3

Abstract

A salmon protein hydrolysate (SPH) was developed containing several angiotensin I-converting enzyme (ACE) inhibitory tripeptides the most abundant of which were Val-Leu-Trp, Val-Phe-Tyr, and Leu-Ala-Phe. Simulated digestion experiments showed that active constituents of SPH would survive in the digestive tract and be available for absorption into the bloodstream. In fact, ACE inhibitory activity was improved following simulated digestion suggesting that there were larger peptides in SPH that might contribute to bioactivity in vivo. A single oral dose (1,500 mg/kg body mass) of SPH significantly lowered blood pressure in spontaneously hypertensive rats (SHR). The treatment of SHR with either SPH fraction (<3,000 Da) or SPH fraction (>3,000 Da) reduced blood pressure. We conclude that the ability of SPH to lower blood pressure is due to a combination of ACE inhibitory tripeptides as identified, as well as additional unknown, peptide species that are generated during digestion of SPH in the gastrointestinal tract.

Keywords

Angiotensin I-converting enzyme inhibitory peptide Blood pressure Nutraceutical Functional food 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • H. Stephen Ewart
    • 1
    • 3
  • Dorothy Dennis
    • 1
  • Michael Potvin
    • 1
  • Christa Tiller
    • 1
  • Lian-hua Fang
    • 2
  • Ran Zhang
    • 2
  • Xiao-ming Zhu
    • 2
  • Jonathan M. Curtis
    • 1
    • 4
  • Sylvie Cloutier
    • 1
  • Guanhua Du
    • 2
  • Colin J. Barrow
    • 1
  1. 1.Ocean Nutrition Canada LtdDartmouthCanada
  2. 2.National Center for Pharmaceutical Screening, Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina
  3. 3.National Research Council of Canada-Institute for Marine BiosciencesHalifaxCanada
  4. 4.Department of Agricultural, Food and Nutritional ScienceUniversity of AlbertaEdmontonCanada

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